涤纶表面接枝大豆蛋白工艺及接枝面科服用性能研究

涤纶织物具有突出的服用性能,但是与人体的亲和性较差,大豆蛋白具有良好的生物相容性.研究了用聚乙醇缩水甘油醚交联剂100%(PVAGE 100%)将大豆蛋白交联接枝在涤纶纤维表面上,制备涤纶接枝大豆蛋白面料.涤纶纤维表面交联接枝大豆蛋白最佳条件:接枝温度90℃.接枝时闻50 min.PVAGE100%浓度33.6 g/L(浴比:1:20).同时研究了涤纶/大豆蛋白复合面料的服用性能.结果表明:接枝率在一定范围内,复合面料吸湿性能有显著的改善,褶皱弹性,透气性、挺括性保持良好.     

涤纶表面接枝大豆蛋白工艺及接枝面料服用性能研究

涤纶织物具有突出的服用性能,但是与人体的亲和性较差,大豆蛋白具有良好的生物相容性.研究了用聚乙醇缩水甘油醚交联剂100%(PVAGE 100%)将大豆蛋白交联接枝在涤纶纤维表面上,制备涤纶接枝大豆蛋白面料.涤纶纤维表面交联接枝大豆蛋白最佳条件:接枝温度90℃,接枝时间50 min,PVAGE100%浓度33.6 g/L(浴比:1∶20).同时研究了涤纶/大豆蛋白复合面料的服用性能.结果表明:接枝率在一定范围内,复合面料吸湿性能有显著的改善,褶皱弹性,透气性、挺括性保持良好.     

Introduction of T-DNA into Watermelon by Stigma Smeared Method and Its Molecular Marker Research

[Objective]The aim was to introduce T-DNA into watermelon for its molecular marker research.[Method]Based on the method of foreign DNA introduced to Arabidopsis thaliana via dipping flowers,the stigma smear was used to transfer T-DNA into watermelon and its molecular marker research was carried out.[Result]The ideal transformed species was ZXG01078 for the highest fruit setting rate and the most deviant seedlings.The best concentration of kanamycin for treating watermelon seeds was 500-700 mg/L with differences among the species.The best position was spire leaf or young leaf and the best concentration of kanamycin for treating the watermelon leaf was 4 000-8 000 mg/L with no significant difference among species.The steadily variation appearing of growing pointless and conjoined twin seedlings indicated that the normal growth had been interfered by foreign DNA in the progeny.[Conclusion]This study had provided basis for the further research on watermelon.     

Hg2+与Cr(Ⅵ)对富营养化水体中藻类生长的毒性效应

[Objective]The toxicity effect of Hg2 and Cr(Ⅵ)on alga growth in eutrophic water was studied to provide reference for biomonitoring and bioremediation of eutrophic water.[Method]The mother liquid of alga,which was separated from the eutrophic water,were put into the solutions of Hg2 and Cr(Ⅵ)with different concentrations and their mixture solution,respectively.And the toxicity effect of Hg2 and Cr(Ⅵ)on the growth and propagation of alga in eutrophic water was observed.[Result]The alga in eutrophic water performed rather sensitive to Cr(Ⅵ)and when its concentration Was over 1 ms/L,threre was obvious effect on alga growth.The alga Was not very sensitive to Hg2 when its concentration was lower, but its toxicity became stronger and stronger when its concentration increased to some extent and the toxicity effect of Cr(Ⅵ)on alga growth was inst on the centrary. When the ion concentration was lower than 10 ms/L,the toxicity of Hg2 on alga was lower than that of Cr(Ⅵ).When the concentration was over 10 ms/L,the toxieity of Hg2 exceeded that of Cr(Ⅵ).The toxicity of ion mixture solution of Hg2 and Cr(Ⅵ)had synergism inhibition on alga,which could be performed only when the concentration was over4 mg/L.[Conclusion]The toxicity of heavy metal on alga not only related to the alga cell,but also related to the concentration of heavy metal ion.     

Screening of Optimal Differentiation Medium toLonicera edulis

An excellent Lonicera edulis strain, L1-8 that was bred by Northeast Institute of Geography and Agro-ecology, Chinese Academy of Sciences, was used as material in this research. The axillary buds of its dormant branches were used as explants. A fourfactor and four-level orthogonal test was designed in order to choose the best differentiation medium for providing the technical support of Lonicera edulis micropropagation. The results showed that the culture medium and concentration of 6-BA were the main factors, and the best differentiation condition was MS culture medium containing 2.0 mg · L-1 6-BA, 0.3 mg · L-1 IBA and 1.5 mg · L-1 GA3.     

Effect of 6-BA and 2, 4-D on Soybean Transformation

Regeneration from cotyledonary nodes and embryonic tips of soybean "Peking" was studied. The disinfectant ways of the mercuric chloride and chlorine gas were used and the concentrations of 6-BA and 2, 4-D were studied in the culture medium. The results showed that the sterilization effect of chlorine gas was better than that of mercuric chloride. The best concentration of 6-BA was 1.0 mg · L-1 and the best concentration of 2, 4-D was 2.0 mg · L-1 in the germinating medium. The number of buds of each explant was 3.56 and 2.98, respectively. The best concentrations of 6-BA and 2, 4-D were 3.0 mg·L-1 and 3.5 mg·L-1 in regeneration of embryonic tips. The best inducing time was 16-20 h and the mean shoots per explant was 2.69 and 2.78, respectively.     

Drying Technology of Angiotensin Converting Enzyme Inhibitory Peptide Derived from Bovine Casein

Drying technology of angiotensin converting enzyme （ACE） inhibitory peptides derived from bovine casein was investigated. No significance was observed on ACE inhibitory activity of products prepared by spay drying and freeze drying （P〉0.05）. Spay drying was the best drying process for practical industry production. The inlet temperature ranged from 140℃ to 160℃ and the exit temperature ranged from 70 ℃ to 90 ℃ during the spay drying process. Under the optimal conditions, scale-up of angiotensin converted enzyme inhibitory peptide from 1 L to 10 L and the experiment was successively conducted. Peptide yield was 29% and half inhibitory concentration （IC50） was 0.53 g. L^-1.     

Expression of Duck Interferon Alpha in BL21（DE3）plysS

To obtain the protein of duck interferon alpha and study its biological activities, the prokaryotic ex-pression vector of DuIFN-α was constructed and expressed in BL21 (DE3) plysS. Using PCR technique, the proteingene of DuIFN-α was cloned from pMD-18-duIFN-α recombinant. The gene was then inserted to pGEM-T vectorand identified by restriction endonuclease analysis and sequencing. DuIFN-α was ligated with the prokaryotic expres-sion vector of pET30 a, then transformed into BL21 (DE3) plysS. The best inducing time and IPTG concentration for the expression of this recombinant protein was tested through the expression of the positive recombinant with differ-ent time span and different IPTG concentration. Lots of the protein of DuIFN-α were expressed in BL21 (DE3)plysS with 1 mmol·L-1 IPTG for 4 hours and its molecular weight for 34 000.     

丁香酚对中国对虾幼虾麻醉效果的初步研究(英文)

[Objective] The aim of the study is to seek a good anesthetic to Fenneropenaeus chinensis. [Method] The anesthetic effect of eugenol to juvenile Fenneropenaeus chinensis was investigated. [Result] The juveniles could be effectively anaesthetized by 50-400 mg/L eugenol aqueous solution with temperature of 24 ℃. Within the concentration range of 50-400 mg/L, the increase of the eugenol concentration could shorten the time required for anesthesia, meanwhile could prolong the time for recovery. The recovered rate of prawn reached 100% when the eugenol concentration was lower than 200 mg/L, while the recovered rate of prawn was just 66.67% when the eugenol concentration was higher than 400 mg/L. The survival rate of prawns in test group was 100% from the observation of three consecutive days. For the specific dose, the anesthetic effect enhanced with the increase of water temperature (18-27℃). [Conclusion] Eugenol is a safe and efficient anesthetics that can be applied in genetic breeding of prawn.     

莱克多巴胺快速检测竞争ELISA试剂盒的研制及其性能测定(英文)

Mixed anhydride(MA)was used to conjugate ractopamine(RAC)to BSA and obtained artificial antigen BSA-RAC identified by UV and SDS-PAGE.Balb/c mice were immunized with BSA-RAC and hybridoma lines that secrete RAC monoclonal antibody(mAb)were generated with cell fusion.A ciELISA kit for detection of RAC(RAC-Kit)was developed with RAC mAb and its performance were tested.The results indicated that BSA-RAC was successfully synthesized and its conjugation ratio of RAC to BSA was about 24.5∶1.Three hybridoma lines were filtered and the best one was 4D8-3E11,its affinity constant(Ka)was 1.65×1010 L/mol.The limit of detection of RAC-Kit was 0.5 ng/ml and its detection range was 0.5-184 ng/ml.The mean recoveries of RAC spiked in feed were 85.6% and in swine urine were 88.6%.The precision and accuracy of the assay as determined by inter-assay and intra-assay coefficient variation were below 15%.It had 9.4% cross-reactivity(CR%)to dobutamine and little or no CR to other compounds.The validity of RAC-Kit in 4 ℃ was in 180 d.     

PAN纳米纤维毡负载TiO2处理废水中苯酚

采用具有光催化活性的TiO2／PAN纳米纤维毡处理模拟废水中的苯酚。结果表明：纺丝液浓度聚丙烯腈（PAN）质量分数为5％的TiO2／PAN纤维毡对苯酚的降解率最高,对浓度100、50、20和10mg／L苯酚的降解率分别为51．1％、87．5％、99．6％和100．0％。光催化反应符合一级反应动力学。纤维毡循环使用5次后对于浓度20mg／L苯酚的降解率保持在87．4％。     

苯酚对废水生物硝化过程的抑制

由于硝化细菌对水产养殖水质可以起到明显调节作用，近年来在水产养殖领域的应用日益广泛。但废水中有毒有害物质的存在常常会导致硝化细菌的活性受到严重抑制，从而严重影响养殖水体及水产品的质量。本文利用富集培养的硝化污泥研究了苯酚对生物硝化过程的抑制效应。结果表明，苯酚对氨氮生物硝化过程的抑制属于非竞争性抑制，抑制常数K1和EC50均为2．61mg／L。泥龄相同的条件下，有苯酚抑制剂存在时氨氮的出水浓度高于无抑制情况，且苯酚浓度越高，氨氮出水浓度也越大；而达到相同的氨氮出水浓度，抑制剂存在条件下的泥龄大于无抑制条件，且抑制程度越高，所需泥龄越长。     

基于GIS的工厂化养殖适宜性评价研究

选取滨海新区汉沽作为研究区域,通过查阅相关文献、咨询水产专家和实地调研等多种形式,筛选出19个对滨海新区汉沽工厂化养殖发展影响较大的因子作为评价指标,综合应用德尔菲法(Delphi)和层次分析法(AHP),确定各级指标的权重,构建滨海新区汉沽工厂化养殖适宜性评价模型,利用IDRISI进行综合运算,将工厂化养殖区域划分为"不适宜"、"一般适宜"、"适宜"和"非常适宜"4种类型,从而为工厂化养殖区域规划和管理提供科学依据。     

基于HTML文档的WEB图像分类研究

随着信息技术的快速发展和Internet的普及应用，Internet已经成为包含各种信息形式，如文本、图像、视频、音频等的巨大信息资源库。然而由于Web图像从主题、格式，到功能和来源都多种多样，很难用一个统一的标准进行分类。本研究首先从Web页面上图像的功能角度对图像进行初步分类，将其分为链接类、装饰类、广告类和内容类。初步分类可提高Web图像处理的效率和性能，并为它基于Web的应用与研究提供帮助。     

苯酚对废水生物硝化过程的抑制

由于硝化细菌对水产养殖水质可以起到明显调节作用，近年来在水产养殖领域的应用日益广泛。但废水中有毒有害物质的存在常常会导致硝化细菌的活性受到严重抑制，从而严重影响养殖水体及水产品的质量。本文利用富集培养的硝化污泥研究了苯酚对生物硝化过程的抑制效应。结果表明，苯酚对氨氮生物硝化过程的抑制属于非竞争性抑制，抑制常数K1和EC50均为2．61mg／L。泥龄相同的条件下，有苯酚抑制剂存在时氨氮的出水浓度高于无抑制情况，且苯酚浓度越高，氨氮出水浓度也越大；而达到相同的氨氮出水浓度，抑制剂存在条件下的泥龄大于无抑制条件，且抑制程度越高，所需泥龄越长。     

转化糖药学研究概况

综述了转化糖药理及其现代临床研究概况,并对其药学应用前景进行了展望。     

布氏艾美尔球虫研究之回顾

布氏艾美尔球虫(Eimeriabrunetti)是鸡的一种致病性较强的寄生性原虫,但是我国球虫流行病学的报道很少谈及,有关研究报道更为少见。为此,笔者回顾了自1942年Levine首次报道布氏艾美尔球虫以来人们对这个种所做的研究。主要对这个种在世界的流行情况、生物学特性、致病性、免疫原性、内生性发育、体外培养、致弱及在球虫疫苗中的地位作了回顾和评述。     

黑龙江省番茄根腐病研究概述

文章对黑龙江省番茄根腐病病原菌的鉴定、病原菌的生物学特性、苗期抗性鉴定技术及寄主抗性遗传规律、番茄根腐病的病症与识别、病害的发生和流行规律等内容进行了综述。