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《中国兽医学报》2019,(12):2467-2471
旨在研究纯中药制剂"益母散"是否对小鼠子宫有直接作用并影响小鼠的发情和雌激素受体的表达。以性成熟雌性小鼠为研究对象,通过手术去除小鼠卵巢,用"益母散"进行灌胃试验,试验期间每天进行阴道涂片,检测小鼠的发情情况,试验结束后取出子宫,测子宫质量,同时对子宫组织HE染色观察子宫的组织形态,通过PCR检测子宫雌激素受体表达,并测定发情期血清雌激素水平。结果表明,"益母散"可明显抑制去卵巢小鼠子宫的萎缩(P0.01),并能促进去卵巢小鼠子宫发育、维持子宫正常组织形态、促进雌激素受体ERαmRNA表达,使去卵巢小鼠出现发情表现。这表明"益母散"具有一定的类雌激素样作用,可通过上调雌激素受体表达,使去卵巢小鼠在内源性雌激素水平较低的情况下出现发情表现。 相似文献
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为了研究钙敏感受体(calcium-sensing receptor,CaSR)基因在大鼠不同组织的相对表达差异,探索其在不同组织上的潜在功能,随机选取7周龄左右的雌性及雄性SD大鼠各5只,采用实时荧光定量PCR技术检测CaSR基因在大鼠胃肠道(胃、十二指肠、空肠、回肠)、代谢器官(肝脏、肾脏)及生殖器官(睾丸、附睾、子宫、卵巢)的相对表达量。结果显示:胃CaSR基因表达量显著高于其他肠段(P0.05);肾脏CaSR基因表达量显著高于肝脏(P0.05);睾丸CaSR基因的表达量显著高于附睾(P0.05);子宫与卵巢的表达量较低,且二者无显著差异(P0.05)。结果表明:CaSR基因在雌性、雄性大鼠的胃肠道、代谢器官以及生殖器官组织中均有表达,且在各组织间的表达存在显著差异。 相似文献
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雌激素是重要的生殖激素之一,其作用的发挥受雌激素受体(estrogen receptor,ESR)介导.因此,研究雌激素受体表达的动态变化规律对阐明鹅卵巢发育和产蛋的分子调控机制具有重要意义.研究利用实时定量PCR技术检测发育期和产蛋期籽鹅卵巢组织中雌激素受体1(ESR1)和雌激素受体2(ESR2)表达的动态变化,为鹅卵巢组织中雌激素及其受体调控机理的研究奠定基础. 相似文献
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促性腺激素受体在雌性水牛生殖器官的表达定位研究 总被引:2,自引:1,他引:1
为研究促性腺激素受体(FSHR、LHR)在广西雌性水牛生殖器官中的分布情况,运用免疫组化SABC法对处于不同发情周期(卵泡期、黄体期)成年水牛的卵巢、子宫、输卵管中FSHR、LHR分别进行染色定位。结果表明,FSHR/LHR阳性细胞在卵巢主要见于卵巢内膜细胞及卵泡颗粒细胞;子宫主要见于子宫内膜上皮细胞和腺体细胞;输卵管主要见于柱状上皮纤毛细胞。其中,随着发情周期不同,FSHR、LHR的表达量也有所差异,卵巢中卵泡期FSHR、LHR的表达量均高于黄体期;子宫中FSHR的表达量卵泡期高于黄体期,LHR的表达量黄体期高于卵泡期;而输卵管并没有显著差异。 相似文献
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雌激素受体和雌性生殖研究进展 总被引:1,自引:0,他引:1
雌激素通过雌激素受体(ER)在雌性生殖活动中起着重要作用,雌激素受体是配体依赖的转录调节因子,属核受体超家族成员。目前认为存在2种亚型:即经典的ERα和新发现的ERβ。利用基因敲除技术研究表明,ERα和ERβ对于卵巢的发育不是必需的,但ERα可通过负反馈回路调节排卵,而ERβ通过卵巢内和卵巢外2条途径调节卵泡的发育和排卵,两者共同作用以维持雌性性征、卵泡发育和排卵等生理活动;在子宫中,ERα占主导作用,在子宫成熟和胚胎附植过程中起着重要作用,ERβ则能以反应特异性和剂量依赖性的方式调节ERα的作用。 相似文献
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促性腺激素释放激素(GnRH)在体内的重要功能是通过促性腺激素释放激素受体(GnRH受体)介导的。在1990年,由Mellon和他的合作者获得了SV40转化的转基因小鼠促性腺细胞系At3-1,为最终获得GnRH受体的分子结构提供了宝贵的试验材料,从而使GnRH及其受体分子调控机制的研究跨入了新的阶段。近年来,GnRH受体的分布、表达、调节等在神经内分泌、生殖生物学领域得到了深入的研究,使人们对GnRH受体的功能有了更深的认识。1GnRH受体的结构GnRH受体由327~328个氨基酸残基组成,有7个跨膜结构域,属G蛋白偶联受体,与其它G蛋白偶联受体不同的是,… 相似文献
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Toll样受体(Toll-like receptors,TLRs)是模式识别受体的主要类别,除表达于经典免疫细胞外,多种哺乳动物的卵巢和生殖道同样存在此类受体分子,其在卵巢活动中发挥着重要作用。TLRs在排卵过程中的生物学功能是生殖免疫学领域研究的主要内容之一。文章主要从TLRs在哺乳动物卵巢中的表达与分布、表达与功能的调节机制及在排卵过程中所发挥的作用及意义等方面进行了综述,并对其在排卵相关疾病中的可能作用进行了简要分析,以期为生殖免疫学相关领域研究提供参考。 相似文献
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能量对初情期前母猪卵巢LHR和FSHR mRNA表达的影响 总被引:2,自引:0,他引:2
对9头50日龄、体质量为30 kg的初情期前杜×长×大三元杂交母猪进行长期日粮能量差异饲养试验.饲养结束后屠宰,取卵巢液氮保存.半定量RT-PCR检测每头母猪卵巢LH和FSH受体mRNA的含量.结果,高能组卵巢LH和FSH受体mRNA的含量显著高于中能组和低能组(P<0.05);而低能组显著低于中能组和高能组(P<0.05).表明,能量水平高的日粮可以显著地促进初情期前卵巢上LH和FSH受体在mRNA水平的表达,而能量水平不足的日粮则不利于这种表达.本文首次报道了日粮能量水平对母猪卵巢LH和FSH受体mRNA表达的影响. 相似文献
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Bang Zhong LIN Shinji SASAZAKI June Heon LEE Hideyuki MANNEN 《Animal Science Journal》2009,80(5):528-531
Growth hormone receptor (GHR) belongs to a member of the cytokine receptor superfamily. Polymorphism of presence or absence of an approximately 1.2 kbp LINE-1 element is observed in bovine GHR gene. The present study was carried out for estimating the genetic diversity and the origin of the LINE-1 element in 10 European, Southeastern Asian and East Asian cattle breeds or populations. Genotyping of the LINE-1 revealed predominant LINE-1 presence in European breeds (0.917∼0.991), absence in the Bos taurus indicus populations (0.000∼0.017), and intermediate presence in Northeast Asian cattle (0.417∼0.522). From genetic features of LINE families, LINE-1 of GHR could be attributed to the same origin in both European and Asian cattle, and Asian LINE-1 may not be derived from recent introgression. This result suggested that LINE-1 in bovine GHR gene could have arisen in an ancestral population of Bos taurus taurus . 相似文献
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为了检测山羊迷走神经结状神经节中是否有促黄体生成素受体(LHR)的存在,探讨促黄体生成素(LH)是否能够影响结状神经节内脏感觉神经元的功能活动。本试验采用免疫组织化学SP法观察LHR在结状神经节中的分布特征,利用IPP 6.0图像半定量分析系统分析LHR在结状神经节中阳性神经元和阳性非神经元上的表达量差异。结果显示,LHR主要分布在神经元细胞膜和细胞质中,呈棕黄色为强阳性。在带核的神经元中,神经元细胞核呈圆形空泡状,LHR为阴性反应。神经元群间的神经纤维呈淡黄色,LHR为弱阳性。LHR在神经元胞体上的相对表达量极显著高于节内其他阳性非神经元结构(P0.01)。结果表明,山羊结状神经节内脏感觉神经元是LH作用的主要靶细胞,提示LH有可能通过作用于结状神经节内脏感觉神经元胞体上的LHR参与调节内脏器官感觉信息的传导。 相似文献
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本文介绍了FSH及其受体的结构,重点讨论了雌性哺乳动物FSH的功能及FSH受体在卵巢中的表达。为进一步的研究工作提供了参考资料。 相似文献
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A. Maj C.S. Pareek M. Klauziska & L. Zwierzchowski 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2005,122(6):414-417
Genes coding for growth hormone (GH) and GH receptor (GHR) are candidates for quantitative trait markers in farm animals. This work describes a search for nucleotide sequence polymorphisms within the 5′‐region of the bovine GHR gene. Two new single nucleotide polymorphisms were found: restriction fragment length polymorphisms (RFLPs) at a Fnu4HI/TseI site (C/T transition at position ?1104), and at a Sau96I site (C/T transition at position ?262). The Fnu4HI/TseI polymorphic site is located within the 1.2‐kbp LINE‐1 retrotransposon upstream of the P1 promoter, while the Sau96I RFLP locates in the P1 promoter for exon 1A. The appearance of the Sau96I RFLP was studied in representatives of two bovine species, Bos taurus and Bos indicus. An absolute correlation was observed between Sau96I genotype and the insertion/deletion of LINE‐1. 相似文献
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Protein and mRNA expression of follicle‐stimulating hormone receptor and luteinizing hormone receptor during the oestrus in the yak (Bos grunniens) 下载免费PDF全文
S‐d Huo S‐e Chen R‐j Long J‐t Yang J‐x Lu R‐x Zang T‐j Zhang A. Abudureyimu J‐l Liu G‐h Zhang Y‐q Zhao Z‐r Ma 《Reproduction in domestic animals》2017,52(3):477-482
Follicle‐stimulating hormone (FSH) and luteinizing hormone (LH) have a central role in follicle growth, maturation and oestrus, but no clear pathway in the seasonal oestrus of yak (Bos grunniens) has been found. To better understand the role of FSH and LH in seasonal oestrus in the yak, six yaks were slaughtered while in oestrus, and the pineal gland, hypothalamus, pituitary gland, and gonads were collected. Using real‐time PCR and immunohistochemical assays, we determined the mRNA and protein expression of the FSH and LH receptors (FSHR and LHR) in these organs. The analysis showed that the FSHR mRNA expression level was higher in the pituitary gland tissue compared with LHR (p < .01) during oestrus. By contrast, there was low expression of FSHR and LHR mRNA in the pineal gland and hypothalamus. FSHR mRNA expression was higher than that of LHR (p < .05) in the ovary, whereas LHR mRNA expression was higher than that of FSHR (p < .01) in the uterus. FSHR and LHR proteins were located in the pinealocyte, synaptic ribbon and synaptic spherules of the pineal gland and that FSH and LH interact via nerve fibres. In the hypothalamus, FSHR and LHR proteins were located in the magnocellular neurons and parvocellular neurons. FSHR and LHR proteins were localized in acidophilic cells and basophilic cells in the pituitary gland, and in surface epithelium, stromal cell and gland epithelium in the uterus. In the ovary, FSHR and LHR protein were present in the ovarian follicle. Thus, we concluded that FSHR and LHR are located in the pineal gland, hypothalamus, pituitary and gonad during oestrus in the yak. However, FSHR was mainly expressed in the pituitary gland and ovaries, whereas LHR was mainly expressed in the pituitary gland and uterus. 相似文献