Differences in Major Ions Composition of Artificial Seawater from Aquarium Tanks at the New Jersey State Aquarium

Concentrations of major seawater ions were monitored in six aquarium tanks at the New Jersey State Aquarium over a three-year period. The ratios of these ions to chlorinity were compared to those in freshly prepared artificial seawater. The largest aquarium tank (Ocean Tank) exhibits statistically significant (p<0.01) relative enrichment of potassium, calcium and strontium, and relative depletion of magnesium and sulphate. The likely source of excess potassium is from potassium iodide added to prevent goiter in sharks. Based on the excess potassium, a total amount of 650 μmol/kg iodide added over the years was calculated for the Ocean Tank. The excess calcium observed in several tanks correlates with the presence of concrete or arbonaceous shells in these tanks. In Ocean Tank, a calcium leaching rate of 6.7 kg/month was calculated. Continuous formation of white carbonaceous precipitates serves as a sink for magnesium in Ocean Tank, and a magnesium removal rate of 5.1 kg/month was calculated. These and other results show that concentrations of major ions in artificial seawater aquaria are affected by leaching, precipitation and addition of food supplements, and these factors should be taken into account when preparing artificial seawater for aquarium tanks with long water residence time. This revised version was published online in August 2006 with corrections to the Cover Date.     

桁拖网捕虾技术推广研究总结

In order to extend the scientific and technical results of beam trawl with double bags for fishing shrimp to the whole fishermen in Haimen county, a summary of the further research of the technigue was given.     

饥饿胁迫对鲤肠道与肝脏蛋白酶和淀粉酶活性的影响

The aim of the present study was to determine the effect of starvation on digestive enzymes activity of the carp （Cyprinus carpio） at the water temperature 18 -20℃ in the cycle and flow water aquarium. The experiment was designed for four groups with three replication,which were 0 day（control） , 10 days, 20 days, 30days group. The results showed That there was a trend to drop first and keep stabilization for Cyprinus carpio body weight and Meat Content with the prolongatin of starvation, and that Effect of starvation stress on the the activity of protease in intestine and hepatopancreas of Cyprinus carpio was significant, with the prolongatin of starvation, there was a trend to increase then drop for the activity of protease in fore-gut, mid-gut hind-gut and hepatopancreas of Cyprinus carpio. And that the variatin for activityof amylase in fore-gut was significant butthat of the others was no significant.     

水产养殖动物基因组研究的现状及其应用前景

The genomic resources from human and several model organisms have been increased very fast since 1990. The techniques for developing genomic resources have already been very advanced and smart. These could make scientists see and improve organism in genomic level. For Chinese aquaculture scientists and aquatic industry, developing genomic resources and genetic tools for the native species are most important in the genomic era. The genomic resources and genetic tools for several aquatic species have been developed and some of them have been used in the marker based selection and other researches. The genome research work on aquaculture species was reviewed in this paper, especially a USDA genome project was focused. Some functional genomic research for aquatic animal was also discussed here. The importance and necessity of China aquaculture species genome project were discussed. Common carp and other cultured fishes in Cyprinidae such as grass carp, silver carp, bighead carp etc were recommended as the candidate species for genome research, because the output of all carps is almost up to 1/3 of total fisheries output in China. Common carp with another virtue for genome research is that there are much more families and strains in common carp than those in other cultured species in China, and those families and strains are the basis for genome research and mapping quantitative trait loci associated with important economic trait. Although the first linkage map of common carp made by Sun needs to be added with more markers for mapping QTL and Type I markers ,it has laid the groundwork for QTL mapping and markerassisted selection in common carp. Because the model organism zebrafish and common carp, grass carp and other carps cultured in China all belong to Cyprinidae, the China carp genome research will obtain a lot of useful information from zebrafish genome research. How the China carp genome program will be conducted and what kinds of strategy involved in this program were all suggested. How the results of the genome research of aquaculture species will be used in the aquaculture industry was reviewed and analyzed here.     

ABSTRACTS AND KEY WORDS

Fisheries Resource Utilization of Main Fishing Gears in the Yellow Sea and Bohai Sea
Abstract fishing capacity stow net, trawl in order to find the Yellow Sea Surveys for catch structure and of main type of fishing gears such as and gillnet were conducted in 2009 out the fishery resource utilization in and Bohai Sea.     

美洲黑石斑鱼营养成分分析与营养价值评价

In this experiment, the contents of the main nutrients such as protein, fat, moisture, ash, calcium and phosphorus were analysed, and the contents of the trace elements, amino acids and fatty acids in its muscle were determined for Centropristis striata. Moreover, its nutritional value was evaluated and compared with other fishes,livestocks and poultries. The results showed that the content of dry matter and protein are higher in its muscle. Then, all of 17 common amino acids（AA） contents in muscle was 17.51% in the fresh sample, and 7 of them are human essential amino acids（EAA）41.58% in the total amino acids（TAA）. The ratio of essential amino acids to nonessential amino acids（EAA/NEAA） was 84.46% ,and the essential amino acids index（EAAI） was 57.71. According to AAS and CS,which basically accorded with the issued standard value by FAO and WHO. The content of 5 delicious amino acids was 44.37% in the TAA, and the ratio of branched chain amino acids to aromatic amino acids was 2.83. The content of polyunsaturated fatty acids was 22.3% rich in its muscle also, in particular EPA and DHA. In conclusion, Centropristis striata is a species of delicious and high nutritional value,which well deserves exploitation and utilization. At the same time, this experiment was preparing for the next study to provide reference data for artificial feed and the expansion of aquaculture.     

Twenty years of cooperation——A brief history of the"Bei Dou"project

A visit to China in September1980 by the then Norwegian Prime Minister,Mr.Odvar Nordli,initiatedthe present cooperation between fisheries institutions in China and Norway. The Prime Minister's delegationwas informed about the programme for economic reconstruction of China,a programme that also included amodernization of the fishing sector. As a support to the open door policy and the new development,Norwayoffered to donate a fisheries research vessel to China.Such a vessel could be used …     

大菱鲆胚胎的玻璃化冷冻保存

Vitrification solutions were examined for their suitability of cryopreservation of turbot (Seophthulnms maximus) embryos. PMPI gave higher survival rate (75.68%) than other vitrification solutions and was considered to be suitable liar the cryopreservation of turbot embryos. The freezing point of the vitrification solutions consisted of PG and MeOH in the proportion of 3:2 was measured when the vitrification solutions were cryopreserved. It showed that the vitrification solutions with a cryoprotectant concentration of over 41% have no freezing point and the freezing points of vitrification solutions containing 33.33%-40% cryprotectants were between -32.4℃ and -65.4℃, The freezing point decreased with the increase of cryoprotectant concentration. The resistance of turbot embryos at different stages to the cryoprotectants was studied, It was found that the turbot embryos from 4-5 pairs somite to tail bud were more resistant to cryoprotectants and suitable to vitrify. A live embryo was obtained after cryopreservation in liquid nitrogen for 14h and was hatched out。     

^60Co-γ射线对成体长牡蛎生长的影响及致死效应

Effects of ionizing irradiation of ^60Co-γ ray on mortality and growth of one year-old Pacific oysters ( Crassotrea gigas ) were studied. Irradiation was conducted at 4℃ and without sea water, and the survival, shell length, shell width, whole wet weight and condition index of irradiated oysters were measured. A biphasic dose response effect of 6^60Co-γ irradiation on Pacific oyster with high stimulatory response dose range to 100 Gy, and strong hormesis effects had been observed. Gamma ray induced mortality happened within 90 days after irradiation, and the relation between dosage ( D ) and mortality at 90 days could be described by the equation of M90 = 0.0041D^2- 0. 1816D (R^2= 0. 9958) with an LD50/90 of 135 Gy, indicating that the Pacific oyster has strong resistance to ^60Co-γ irradiation. Growth in shell length, shell width and whole wet weight of Pacific oyster were also enhanced by 60 Gy of ^60Co-γ ray. The condition index was also enhanced by ^60Co-γ irradiation in the dosage range from 40 to 100 Gy, with the maximum stimulatory response of 2.5 fold of control value at the 40 Gy dosage, suggesting that the accelerating effects of γ ray on growth of the Pacific oyster led to increase in dry meat. Results of this study showed that ionizing irradiation might be useful in the improvement of production traits in shellfish.     

水产品中三聚氰胺残留的测定研究

method for determination of melamine residue in fishery products by liquid chromatography-mass spectrum （LC-MS/MS）was established. The conditions of LC-MS/MS was optimized. The sample was ultrasonically extracted with 1% triehloroactic acid and ethylamine, and cleaned by the solid phase extraction （MCX） cartridge. The analyte was identified by electrospray ionization （ESI） in positive mode using multiple reaction monitoring （MRM）. The determination ion pairs are 127/85 and 127/68,and the confirmation ion pairs are 127/85. The method was validated and good results were obtained with respect to spiked recoveries. The linear range was from 10 to 400 ng/mL,the average recoveries were between 80.5%and 94.1% in the spiked rang of 0.01 -10.0 mg/ kg. LOQ was 0.01 mg/kg.     

以遥感夜间灯光数据为基础的西北太平洋秋刀鱼渔船识别

研究和开发利用大洋性生物资源是实现我国海洋渔业可持续发展的重大战略需求,西北太平洋秋刀鱼是近年来我国重点开发的大洋性渔业资源之一,因此利用卫星来监测渔船时空分布动态成为了解秋刀鱼渔业资源变动的重要数据源。本研究采用峰值检测和阈值分割等方法对西北太平洋夜间灯光数据进行识别,利用地理信息系统技术对渔船位置信息和数量进行提取分析。通过西北太平洋秋刀鱼资源调查的渔捞日志和经过筛选的北太平洋渔业委员会(NPFC)渔船列表数据对识别结果进行验证。结果显示,本研究所用的夜间灯光渔船识别方法可以精确识别西北太平洋密集作业及外围分散作业的秋刀鱼渔船。以此为基础可以有效地分析秋刀鱼渔场的时空变动。结合美国国家海洋和大气管理局(NOAA)提供的海表温度(SST)数据绘制等温线,进一步分析作业渔场的时空变化,发现夜间灯光渔船作业的温度范围随着秋刀鱼洄游而变化。2016年7—9月渔场的SST波动较大是因为这一时期秋刀鱼在黑潮—亲潮广泛的交汇区域洄游,分布更为广泛,9月之后作业渔场SST变动趋于稳定。该研究结果将来会对远洋渔场环境实时变化、鱼群分布预测、渔船动态及法律支撑等提供有效信息。     

中心渔场智能预报系统的设计与实现

应用人工智能技术设计中心渔场智能预报系统,包括系统的总体结构,范例库的建设,范例推理、规则库和规则修正,最后按设计路线给出试验性预报实例,预报结果与渔场实际情况比较,预报的准确性为７６．２％。研究结果表明,采用范例推理为主,规则修正为辅的技术路线使用计算机实现中心渔场智化预报是可行的。它可以为海洋渔业生产和管理部门提供快速、准确、实用的信息预报。     

基于贝叶斯原理的大洋金枪鱼渔场速预报模型研究

高度洄游的大洋性金枪鱼类（Scombridae）是世界远洋渔业的重要捕捞对象,中国金枪鱼生产仍处于初期发展阶段,因此研究和预测金枪鱼渔场具有重要的现实意义.本研究利用美国NASA提供的卫星遥感反演海表温度（SST）三级数据产品和太平洋共同体秘书处（SPC）提供的有关国际金枪鱼历史捕捞产量资料,分析金枪鱼同SST等海洋渔场环境要素之间的统计关系,建立了金枪鱼渔场的贝叶斯概率预报模型.通过对历史数据进行模型回报试验,结果表明太平洋鲣鱼渔场综合预报的准确性达到70%以上,对渔业捕捞生产具有一定的指导意义.[中国水产科学,2006,13（3）：426-431]     

东南太平洋智利竹筴鱼中心渔场的月间变动研究

根据2003年～2011年中国东南太平洋智利竹筴鱼（Trachurus murphyi）大型拖网渔捞数据和海表温度（SST）数据,利用ArcGIS 10.0绘制渔场产量重心时空分布图,用最短距离法对中心渔场进行聚类,对智利竹筴鱼渔场月间重心变动规律及其与SST之间的关系进行分析。结果表明,随着月份的变化,中心渔场从3月开始由南逐渐向西北方向移动,到10月之后逐渐向东南方向移动。3月～6月渔场主要分布在80°W～90°W、40°S～45°S,最适宜SST为12～14℃;7月～8月渔场主要分布在80°W～90°W、35°S～40°S,SST为13～14℃;9月～10月渔场分布为85°W～95°W、30°S～35°S,SST这15～16℃;11月～12月渔场分布在90°W～95°W、30°S～40°S,SST为16～17℃;而1月～2月渔场分布在85°W～95°W、35°S～45°S,最适宜SST为16～17℃。     

2015年超强厄尔尼诺事件对西北太平洋柔鱼渔场变动的影响

为研究超强厄尔尼诺事件对西北太平洋海域柔鱼（Ommastrephes bartarmii）资源量变动的影响,并分析柔鱼栖息地在极端气候条件下的变化规律,根据上海海洋大学鱿钓科学技术组提供的中国柔鱼生产捕捞数据,比较2008年正常气候年份与2015年超强厄尔尼诺年份的单位捕捞努力量渔获量（CPUE）、产量、捕捞努力量以及渔场纬度重心（LATG）的变化;利用栖息地适宜性指数模型对西北太平洋柔鱼栖息地的海表温度（SST）、光合有效辐射范围（PAR）和海表面高度距平（SSHA）3个关键环境因子进行分析。渔业数据时间为2008年和2015年9—11月,数据覆盖范围为36°N~48°N、150°E~170°E。结果发现,相对于2008年正常年份,2015年超强厄尔尼诺事件下的CPUE明显降低,且LATG向南偏移;此外,2015年适宜的SST和PAR范围均显著降低,导致适宜的栖息地面积与正常年份相比大幅减少;最适宜的SST和PAR等值线向南偏移,导致有利的栖息地纬度位置向南移动。研究认为,2015年超强厄尔尼诺事件发生时,柔鱼渔场环境不适于柔鱼生长,适宜栖息地面积减少且向南移动,导致该年份柔鱼资源丰度骤减,渔场向南偏移。     

西北太平洋公海7~9月秋刀鱼渔场分布及其与水温的关系

根据2003~2005年7~9月西北太平洋公海秋刀鱼生产数据和水温遥感数据,对西北太平洋公海秋刀鱼作业渔场分布及其与表温和上层水温结构的关系进行了分析。结果认为,随着时间的推移,7~9月西北太平洋公海秋刀鱼渔场重心有从西南向东北变动的趋势;各月产量重心处水温结构有较大差异,9月混合层深度较7月和8月有所加深,渔场也较后者向北移动;各月高产渔区0~15m温度梯度都在0.25℃/m以下,0~40m温度梯度在0.1℃/m左右,40~60m温度梯度在0.25~0.42℃/m之间。灰色关联度分析表明,渔区月产量受到众多因素的影响,其中捕捞努力量、渔区平均日产量和表温是其主要影响因子,对渔区平均日产量影响较大的有表温、0~15m温度梯度、0~40m温度梯度和月份,其关联度都在0.80以上。     

基于贝叶斯概率的印度洋大眼金枪鱼渔场预报

本文采用贝叶斯概率为模型基础框架,利用来自印度洋金枪鱼管理委员会(IOTC)的大眼金枪鱼延绳钓历史渔获统计数据和美国国家海洋大气管理局(NOAA)的海温最优插值再分析数据,进行适用于印度洋金枪鱼延绳钓渔场的模型参数估算与预报模型构建。模型回报精度验证结果表明,印度洋大眼金枪鱼延绳钓渔场综合预报的准确率达到了65.96%。模型预报结果用概率百分比来表示,符合渔业资源分布的客观特点。利用中分辨率成像光谱仪MODIS提供的SST产品进行业务化运行的渔场预报,利用模型结果每周生成印度洋大眼金枪鱼延绳钓渔场概率预报图,用不同大小的圆形来表示渔场概率的高低,可以为印度洋区域的远洋渔业生产提供信息支持。     

基于GAM和权重分析的西北太平洋秋刀鱼渔情预报研究

为了提高秋刀鱼(Cololabis saira)渔情预报模型的时空分辨率, 提升生产经济效益, 本研究基于 2013─2016 年 7—11 月中国在西北太平洋公海的秋刀鱼生产数据及海洋环境数据, 利用广义可加模型(generalized additive models, GAM)分别拟合单位捕捞努力量渔获量(catch per unit effort, CPUE)的适宜性指数(suitability index, SI)与各海洋环境变量之间的 SI 模型, 结合提升回归树模型(boosting regression tree, BRT)进行权重分析, 建立以月份为周期的秋刀鱼栖息地适宜性指数(habitat suitability index, HSI)模型。结果表明, (1) GAM 能较好地拟合适宜性指数与环境变量的关系, 获得最优环境变量参数值;(2) 环境变量对 CPUE 影响权重的前 3 位分别为海表温度梯度、海表温度和混合层深度, 其中, 在秋季 9—11 月海表温度梯度的权重值均为最高;(3) HSI 模型的检验和评价总体准确率分别为 82.0%和 73.2%, 秋季可达 87.7%和 77.9%, 在盛渔期 10 月, 预测准确率达 89.4%;(4) HSI 高值区与秋刀鱼实际渔场在空间分布基本一致。研究表明该模型适用于秋刀鱼的渔情预报, 并在每天的速报中具有明显优势。     

环境因子对东南太平洋智利竹䇲鱼渔场时空分布异质性影响

基于2012—2018 年4—8 月我国东南太平洋智利竹?鱼 (Trachurus murphyi) 渔捞日志数据,应用地理权重回归模型 (GWR) 探究智利竹?鱼渔场资源分布与环境因子的空间异质性关系.结果表明,环境因子海面温度基于GWR 模型回归的拟合优度为0.54,校正的拟合优度为0.34,赤池信息准则 (Aka...     

基于灰色系统的北太平洋柔鱼渔汛特征分析及旺汛期预测

柔鱼(Ommastrephes bartramii)是大洋性经济头足类,是我国远洋鱿钓渔船重要的捕捞对象。分析柔鱼渔汛特征并预测旺汛期,有助于柔鱼资源的合理开发与利用。本研究根据2013―2017年北太平洋柔鱼渔业生产统计数据,以每日平均渔获量(CPUEday)作为资源丰度,利用分位数的方法划分旺汛期;结合灰色波形预测方法,对旺汛期日期序列建立灰色波形预测模型群[GM(1,1)模型],对旺汛期出现的时间进行预测。结果显示,北太平洋柔鱼渔汛时间最早为5月12日,一直持续到年终;旺汛期为每年的8―11月,第1旺汛期基本在8月出现。GM(1,1)模型的平均相对误差为6.83%,旺汛期日期序列预测的平均相对误差为8.19%,验证数据的平均相对误差为15.82%,表明此模型可预测北太平洋柔鱼的旺汛期。研究结果可为远洋渔业企业的高效率、合理化的科学生产提供技术支撑。