一氧化氮对小鼠卵母细胞自发成熟的影响及作用机制

为研究一氧化氮（NO）供体硝普钠（nitroprusside sodium，SNP）对小鼠卵母细胞体外自发成熟的作用，并进一步探讨NO影响卵母细胞体外自发成熟的可能机制，我们采用了体外细胞培养方法和放射免疫分析法（RIA）。细胞培养研究结果显示，NO供体SNP（1mM）能够延迟卵丘卵母细胞复合体(cumulus-oocyte complexes, COCs) 自发成熟过程中GVBD的发生，抑制PB1的释放。放射免疫分析（RIA）实验结果表明，1mM SNP能够显著的升高COCs内cGMP的水平，而可溶性鸟苷酸环化酶（sGC）抑制剂ODQ（10μM）能够消除SNP对cGMP水平升高的促进作用。同时10μM ODQ还能够逆转SNP对卵母细胞自发成熟的抑制作用，而PKG抑制剂KT5823（1μM）却不能够逆转SNP对COCs自发成熟的抑制作用。以上结果说明NO是通过激活sGC，提高细胞内cGMP水平来发挥其作用的，但cGMP下游的PKG信号通路并不参与NO调控的COCs自发成熟过程。     

用冻干精子生产ICSI小鼠

前期研究中，我们采用小鼠卵母细胞胞浆内精子注射（ICSI）技术，成功地用新鲜精子获得了生理健康的ICSI小鼠。在此基础上，本文结合细胞冻干技术，进行了冻干精子ICSI生产试管小鼠的尝试。B6D2F1成年公鼠的精子在Tris-HCl-EDTA（pH8.2）溶液中冻干4 h后解冻，将其精子头注入到KM小鼠成熟卵母细胞的胞质中。6 h后，83.0%的卵子受精。用CZB溶液体外培养发现，冻干精子生产的胚胎，体外发育至2-C (92.0% vs 99.5%)、4-C（52.7% vs 97.2%）、桑椹胚（36.6% vs 86.3%）和囊胚的比例（21.4% vs 68.7%）极显著地（p<0.01）低于新鲜精子生产的ICSI胚胎。移植2-C期冻干精子ICSI胚胎检测体内发育，结果发现，D10的着床率为63.0%（17/27）；胎鼠的出生比例为21.7%（13/60），这些ICSI小鼠成年后的生理和生殖能力正常。试验结果说明，精子冻干后，仍能生产ICSI动物，冻干技术可以用于哺乳动物精子的保存。     

铝、镉、汞和铅对鸟的慢性毒理作用

<正> 铝(Al)新陈代谢及毒理学Al 直接的潜在毒性比其他金属低,在啮齿类动物腹膜内注射 Al(NO_3)_3·9H_2O,其半致死量(LD_(50))约是320mg/kg 体重。而 ZnSO_4·6H_2O、CuSO_4·5H_2O 和 Hg(NO_3)_2的半致死量(LD_(50))分别为40—50mg/kg、30mg/kg 和 8mg/kg。由口摄食 Al 的慢性毒性对体内 Ca 和 P的内环境稳定的破坏性毒性影响远比 Al 直接引起的细胞毒性大。饮食中0.1%以下的Al一般对 Ca 和 P 的新陈代谢即动物的整体健康没有不利影响。但较高含量的 Al 可能引起生长率降低并伴有以 Ca、P 代谢失调为标     

山羊卵母细胞无血清体外成熟培养研究

摘要：为了研究无血清培养系统对山羊卵母细胞体外成熟及孤雌激活胚胎早期发育力的影响，分别以0.3% BSA(m/v)、1% PVA(m/v)、1% ITS(v/v)代替成熟培养液中FBS，以添加5% FBS的OM为对照组，对来源于屠宰场的COCs进行体外培养。成熟率PVA添加组（50.00%），显著低于FBS组（83.23%）（P﹤0.01），BSA组（71.19%）和ITS组（76.79%）与FBS组无显著差异（P＞0.05）；成熟卵母细胞用离子霉素联合6-DMAP激活，在SOFaa中进行孤雌胚培养，BSA、PVA、ITS及 FBS各组的卵裂率依次为61.90%、46.97%、79.07%和83.58%，囊胚率分别为25.00%、19.70%、55.81%和60.45%。实验结果指出:在OM基础培养液中添加BSA或PVA卵母细胞的成熟率明显低于添加FBS，且卵裂率和囊胚发育率也低于对照组(P<0.05或 P<0.01)；添加ITS，卵母细胞成熟率虽低于对照组，但孤雌胚卵裂率和囊胚发育率与对照组接近（P>0.05）。结论：以ITS代替FBS，可以用于山羊卵母细胞体外成熟培养。     

超表达线粒体融合蛋白2基因(Mfn2)抑制小鼠卵母细胞体外成熟

线粒体融合蛋白2基因(mitofusin2,Mfn2)参与细胞凋亡和细胞周期调控,在卵母细胞和胎盘发育中起着重要作用.本研究旨在探讨Mfn2基因对小鼠(Mus musculus)卵母细胞体外成熟过程的影响.从小鼠卵巢中克隆Mfn2基因并构建pMfn2-Venus真核表达载体.经脂质体2000介导重组质粒pMfn2-Venus转染293T细胞(病毒包装细胞),确认重组质粒在293T细胞内的表达及定位.将pMfn2-Venus体外转录为cRNA,并注射入小鼠卵细胞中,进行体外成熟培养,并统计卵母细胞生发泡破裂(germinalvesiclebreakdown,GVBD)发生率以及第一极体(first polarbody,PB1)排出率,荧光显微镜下观察其表达及定位.结果显示,注射了Mfn2-Venus cRNA的卵母细胞,其GVBD发生率以及PB1排出率与对照组相比显著降低(P＜0.0001,P＜0.05).本研究首次揭示了Mfn2基因对小鼠卵母细胞体外成熟过程的影响,为卵母细胞体外成熟的研究提供了一个新方向,同时也为研究减数分裂过程中Mfn2基因提供了一个平台.     

中草药添加剂的抗小鼠腹泻效果及其对小鼠肠道微生物菌群的影响

为研究中草药提取液的抗小鼠腹泻效果及其对小鼠肠道菌群的影响,本实验选用50只体重为18～22 g的清洁级ICR(Institute of Cancer Researceh,USA)小鼠(Mus musculus),随机分为5个处理组:空白对照组、复方提取液组、黄柏提取液组、白头翁提取液组和白头翁:黄柏(1:1)提取液组.每天按照4 g生药/kg体重剂量定时灌胃,空白对照组给予等剂量的蒸馏水.第10天每只小鼠按0.02 mL/g体重剂量腹腔注射108 cfu/mL大肠杆菌(Escherichia coli)菌液,建立小鼠细菌性致腹泻病理模型,观察各提取液对小鼠的腹泻的影响,第13天收集粪样,变性梯度凝胶电泳(DGGE)研究肠道微生物区系的变化.结果显示,造模当天对照组的体重变化最为明显,腹泻最为严重,与其他各给药组相比均差异显著(P＜0.05);4个中草药提取液添加组小鼠的腹泻次数比对照组分别降低了51％、26％、33％和35％,其中复方组抑制小鼠的腹泻效果最为显著(P＜0.05);小鼠粪便微生物DGGE图谱分析显示,复方组的条带数和多样性指数均显著高于其他处理组(P＜0.05).研究结果表明,中草药添加剂能够显著地抑制小鼠腹泻,有效地改善肠道微生物菌群结构,其中复方组的效果最优.     

滇池流域集约化菜田NO与NO_2排放的研究

采用密闭通气气室法,在滇池流域旱季和雨季开展了2个生长周期内集约化西芹地NO/NO2排放研究。结果表明,NO/NO2排放速率的日变化规律受温度的影响较为明显,中午时段最高,凌晨时段最低。西芹生育期间,CK处理（裸地）的NO/NO2排放速率维持在一定水平,中后期NF处理（不施氮）NO/NO2排放速率有所升高;LF（N450 kg/hm^2）和HF（N 1200 kg/hm^2）处理受西芹的生长和频繁氮肥追施的影响,生育期NO/NO2排放速率逐渐升高。旱季与雨季CK处理NO/NO2排放量分别为1.30和NOx-N 1.51 kg/hm^2,NF处理分别较CK高出NOx-N 1.0和1.44kg/hm^2。LF处理旱季与雨季NO/NO2排放量分别为NOx-N 4.88和5.67 kg/hm^2,其损失率分别为0.79%和0.92%;HF处理旱季和雨季NO/NO2排放量分别为NOx-N 7.58和10.19 kg/hm^2,其损失率分别为0.63%和0.85%,说明氮肥用量较高时,土壤-作物系统的NOx-N损失量也较高,但其损失率并不随施氮量的升高而升高。     

滇池流域集约化菜田NO与NO2排放的研究

采用密闭通气气室法,在滇池流域旱季和雨季开展了2个生长周期内集约化西芹地NO/NO2排放研究。结果表明, NO/NO2排放速率的日变化规律受温度的影响较为明显,中午时段最高,凌晨时段最低。西芹生育期间,CK处理（裸地）的NO/NO2排放速率维持在一定水平,中后期NF处理（不施氮）NO/NO2排放速率有所升高;LF（N 450 kg/hm2）和HF（N 1200 kg/hm2）处理受西芹的生长和频繁氮肥追施的影响,生育期NO/NO2排放速率逐渐升高。旱季与雨季CK处理NO/NO2排放量分别为1.30和NOx-N 1.51 kg/hm2,NF处理分别较CK高出NOx-N 1.0和1.44 kg/hm2。LF处理旱季与雨季NO/NO2排放量分别为NOx-N 4.88和5.67 kg/hm2,其损失率分别为0.79%和0.92%;HF处理旱季和雨季NO/NO2排放量分别为NOx-N 7.58和10.19 kg/hm2,其损失率分别为0.63%和0.85%,说明氮肥用量较高时,土壤—作物系统的NOx-N损失量也较高,但其损失率并不随施氮量的升高而升高。     

猪卵母细胞在不同条件下的体外成熟培养

研究了不同时间段添加外源激素及不同基础培养液和血清有无对猪卵母细胞体外成熟的影响。实验包括（1）猪卵母细胞以TCM199＋10％FBS＋10IU／mL pregnant more serum gonadotrophin（PMSG）＋10IU／mL human chorion gonadotrophin（hCG）＋2．5IU／mL follicle stimulate hormone（FSH）为成熟培养液体外培养44h，前22h在培养液中加激素，后22h不加激素及前22h不加激素，后22h添加激素和添加激素连续培养44h；（2）以实验（1）中激素添加的方案在体外添加激素连续培养44h，对不同基础培养液（TCM199粉剂、TCM199原液和改良TCM199（mM199））对猪卵母细胞体外成熟进行了研究；（3）以实验（2）中筛选出的mM199组为成熟培养液对比血清有无对猪卵母细胞体外成熟的影响。结果表明，实验（1）中3种激素添加不同时间段其成熟率分别为45．67％、45．29％和46．07％，对猪卵母细胞体外成熟无显著影响（P〉0．05）；实验（2）中，mM199组的成熟率（54．10％）高于TCM199粉剂组的成熟率（46．16％）及TCM199原液组的成熟率（47．14％），但差异不显著（P〉0．05）；实验（3）中无血清组的成熟率（67．10％）高于有血清清组的成熟率（52．22％），差异显著（P〈0．05）。由此表明，mM199＋10IU／mL PMSG＋10IU／mL hCG＋2．5IU／mL FSH是一种适合于猪卵母细胞体外成熟的培养液，体外成熟率达67．10％。     

小鼠极体重建卵母细胞的体外受精与发育

本实验分别以昆明系、ICR系和C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb系小鼠（即转绿色荧光蛋白基因小鼠,简称荧光标记小鼠）为主要试验动物,探索其卵母细胞第一极体核重组卵母细胞的生殖功能。超数排卵后采集小鼠卵丘卵母细胞复合体（COCs）,利用链霉蛋白酶酶解法分离出第一极体（PbI）;以形态学和台盼蓝染色相结合的方法对不同温度保存条件下COCs中PbI进行活力鉴定和分级;采用显微操作术将PbI核供体注射到去核卵母细胞中,卵母细胞,进一步观察重建卵母细胞体外受精和体外培养发育能力。结果表明：注射hCG后12～14h回收的PbI活力最佳, 4℃条件下保存48h 后PbI仍保持活性;117枚昆明系和ICR系小鼠重建卵母细胞经体外受精获得13枚2-细胞胚胎, 38枚荧光标记小鼠的卵母细胞中有3枚卵裂成2-细胞胚胎。本研究初步证明小鼠第一极体重建卵母细胞可持续随后受精及其早期发育过程,为发掘和利用其它哺乳动物母本基因资源提供了参考依据。     

哺乳动物卵母细胞核质成熟的差异及其影响因素

摘要：从体内成熟与体外培养、激素处理或不处理以及在培养液中添加某些影响因子对哺乳动物卵母细胞核质成熟差异的现有研究资料进行了综述。     

不同成熟液对猪卵母细胞体外成熟和体外受精后早期胚胎发育的影响

本研究探讨了不同成熟液[改良TCM-199液(mTCM)和改良NCSU-23液(mNCSU)]对猪卵母细胞体外成熟和体外受精后早期胚胎发育的影响，以期提高其受精和胚胎发育潜能。试验表明：(1)猪卵母细胞的核成熟与其周围的卵丘细胞扩展没有必然的联系，卵丘细胞扩散或成放射状不宜作为猪卵母细胞核成熟的标准，只有排出第一极体才能作为猪卵母细胞核成熟的标志；(2)利用mTCM 10％猪卵泡液和mNCSU 10％猪卵泡液的猪卵母细胞体外培养核成熟效果优于mNCSU 10％胎牛血清，但这三组成熟液对猪卵母细胞的卵丘细胞扩散和体外受精后的早期胚胎发育无显著影响。     

Inhibition of reactive nitrogen species effects in vitro and in vivo by isoflavones and soy-based food extracts

Recent studies have shown that soy isoflavone inhibits inducible nitric oxide (NO) synthase activities and is reported to have peroxynitrite scavenging ability. Consequently, we investigated whether isoflavones (daidzein and genistein) and extracts from soy-based products (miso, soymilk, tofu, soy sprout, black soybean, soybean, and yuba) would inhibit the reactive nitrogen species (RNS) effect in vitro and in vivo. In the in vitro experiments [including the protection of cellular DNA from peroxynitrite or sodium nitroprusside damage, an inhibitory effect on nitric oxide production from lipopolysaccharide (LPS)-induced RAW 264.7 cells, and nitric oxide scavenging ability], extracts from soy-based foods showed a potent antioxidant activity and an inhibiting effect on RNS activity. These effects were correlated with total isoflavone content. In the in vivo experiments, rats were given isoflavones (4.0 mg/kg bw) or soy-based product extracts (1.0 g/kg bw) orally for 1 week and were injected with vehicle H(2)O (1 mL/kg bw) or LPS (10 mg/kg bw) on the day 7. Twelve hours after treatment, the rats were killed, and blood serum was collected for analysis. The intraperitoneal administration of LPS resulted in an increase in serum nitrite, nitrate, and nitrotyrosine concentrations. These are stable metabolite end products of nitric oxide, to 4-, 16-, and 5-fold levels, (4, 10 microM and 58 +/- 14 pmol/mL), of the placebo control, respectively. Results showed that oral administration of isoflavones and extracts from soy-based products significantly decreased serum nitrite, nitrate, and nitrotyrosine levels in LPS-induced rats. This study demonstrates that soy isoflavone supplementation may inhibit RNS-induced oxidation both in vitro and in vivo.     

Differential effects of garlic oil and its three major organosulfur components on the hepatic detoxification system in rats.

The objective of this study was to compare the modulatory effect of garlic oil and its three organosulfur compounds, diallyl sulfide (DAS), diallyl disulfide (DADS), and diallyl trisulfide (DATS), on rat hepatic detoxification enzyme activity, and protein and mRNA expression. Rats were orally administered garlic oil (80 or 200 mg/kg bw), DAS (20 or 80 mg/kg bw), DADS (80 mg/kg bw), or DATS (70 mg/kg bw) three times a week for 6 weeks. Control rats received corn oil. According to the results, garlic oil and DAS in dosages of 200 and 80 mg/kg bw, respectively, significantly increased pentoxyresorufin O-dealkylase (PROD) activity as compared with the that of the control rats (P < 0.05). In contrast, N-nitrosodimethylamine demethylase activity in rats that received DADS and DATS was significantly lower than that in the control rats (P < 0.05). Ethoxyresorufin O-deethylase and erythromycin demethylase activities were not influenced by garlic oil, DAS, DADS, or DATS. To the phase II enzyme, garlic oil, DADS, and DATS significantly increased the glutathione S-transferase (GST) activity toward ethacrynic aicd (P < 0.05). Immunoblot assay showed that the protein contents of cytochrome P450 1A1, 2B1, and 3A1 were increased by garlic oil and each of three allyl sulfides, and the change among the allyl sulfides was in the order of DAS > DADS > DATS. The placental form of GST (PGST) level was also increased by garlic oil and the three allyl sulfides, but the increase among the allyl sulfides was DATS congruent with DADS > DAS. P450 2E1, however, was suppressed by each garlic component. Northern blot results indicated that the changes in P450 1A1, 2B1, 3A1, and PGST mRNA levels by garlic components were similar to those noted in the protein levels. These results indicate that the modulatory effect of garlic oil on hepatic drug-metabolizing enzymes can be attributed to its three major allyl sulfide components DAS, DADS, and DATS. These three allyl sulfides vary in modulatory activity, and this variation is related to the number of sulfur atoms in the molecule.     

Antioxidant activity of vinegar produced from distilled residues of the Japanese liquor shochu

Rice shochu distilled residue (RSDR) is a byproduct of rice shochu production. RSDR was converted into vinegar by acetate fermentation. In our present study, two major antioxidant compounds, tyrosol and ferulic acid, were identified from the RSDR-derived vinegar. Furthermore, we investigated the antioxidant activity of freeze-dried RSDR-derived vinegar, which was Acetobactor aceti fermentation powder (AFP), in vitro and in vivo. AFP at 0.25 mg/mL or higher concentrations showed an inhibitory effect against lipid peroxidation and cellular GSH depletion in HepG2 cells induced by H(2)O(2) (P < 0.05). We thus considered the potential of AFP in protecting cells against damage induced by H(2)O(2). Its antioxidant activity was evaluated in vivo using carbon tetrachloride (CCl(4))-induced acute liver injury mouse models. Five consecutive days of oral preadministration of AFP dissolved in PBS at 200, 400, and 800 mg/kg body weight significantly suppressed lipid peroxidation in the liver induced by CCl(4) (P < 0.01). Consequently, treatment with AFP at 200 mg/kg body weight or higher doses suppressed the elevation of alanine aminotransferase and aspartate aminotransferase levels in serum (P < 0.05). These findings suggest that RSDR-derived vinegar can be developed as a health food with an antioxidant effect for the prevention of oxidative injury and cancer.     

Effect of garlic oil on neutrophil infiltration in the small intestine of endotoxin-injected rats and its association with levels of soluble and cellular adhesion molecules

Garlic ( Allium sativum ) possesses anti-inflammatory effects. This study investigated the effects of garlic oil on endotoxin-induced neutrophil infiltration in the small intestine. Wistar rats received by gavage 10, 50, or 100 mg/kg body wt garlic oil (GO) or the vehicle (corn oil; 2 mL/kg body wt) every other day for 2 weeks before being injected with endotoxin (ip, 5 mg/kg body wt). Control rats were administered corn oil and injected with sterile saline. Blood samples for the measurement of soluble adhesion molecules were collected at various time points after injection, and all other samples were collected 18 h after injection. The 10 and 50 mg/kg doses suppressed endotoxin-induced neutrophilia, serum levels of sL-selectin and sICAM-1, cellular CD11b on neutrophils, intestinal ICAM-1 content, and neutrophil infiltration (P < 0.05). The 100 mg/kg dose significantly lowered local ICAM-1 and cellular CD11b on neutrophils (P < 0.05) but did not have a beneficial effect on neutrophil infiltration. In addition, 100 mg/kg of GO worsened the elevation of the local TNF-α level and neutrophilia. Appropriate doses of garlic oil have a preventive effect on endotoxin-induced neutrophil infiltration and damage to the small intestine.     

小鼠卵丘细胞对牛卵母细胞体外成熟和孤雌发育的影响

本实验通过牛生发泡(GV)期无卵丘细胞卵母细胞-裸卵(denuded oocytes,DOs)与小鼠卵丘细胞(mouse cumulus cells,mCCs)共培养,研究异种mCCs对牛DOs体外成熟和孤雌激活后发育的影响,进而探讨卵丘细胞对卵母细胞的作用机制,为牛卵母细胞体外成熟效率提高提供实验依据.牛卵母细胞分为COCs(cumulus-oocytes complexes)组、DOs+bCCs(bovine cumulus cells,bCCs)组、DOs+mCCs组和DOs组4个组合,体外成熟22～24h后,检测各组卵母细胞第一极体排出率、MⅡ期卯母细胞中谷胱甘肽(glutathione,GSH)含量利孤雌激活后的发育能力,以及卵母细胞内骨形态发生蛋白15( BMP15和生长分化因子9(GDF9)mRNA相对表达水平.结果表明:(1) DOs+mCCs组和DOs+bCCs组卵母细胞核成熟率[(68.48±5.71)％,(71.00±4.27)％]显著低于COCs组(84.70±3.41)％(P＜0.05),但均显著高丁DOs组(54.26±5.03)％(P＜0.05); (2) DOs+mCCs组MⅡ卵母细胞GSH含量和孤雌激活囊胚率[(2.48±0.24) pmol/oocyte,(44.19±4.03)％]与DOs+bCCs组[(2.49±0.18)pmol/oocyte,(48.57±5.20)％]和DOs组[(2.40±0.17)pmol/oocyte,(43.47±7.34)％]之间没有差异,但分别显著低于COCs组[(9.67 ±0.18)pmol/oocyte,(59.93±6.13)％](P＜0.05),各组孤雌激活卵裂率之间没有显著差异[(98.85±4.32)％,(92.11±2.00)％,(95.40±4.11)％,(93.18±3.19)％](P＞0.05);(3)DOs+mCCs组、DOs+bCCs组和COCs组卵母细胞中BMP15和GDF9 mRNA相对表达水平均显著性高于DOs组(P＜0.05),其中,DOs+mCCs组和COCs组GDF9 mRNA相对表达水平显著高于DOs+bCCs组(P＜0.05).实验结果表明,mCCs能提高牛DOs的核成熟率及BMP15和GDF9 mRNA相对表达水平,但对牛DOs的细胞质成熟和MⅡ卵母细胞孤雌激活胚胎囊胚发育没有促进作用,说明卵丘细胞通过一些旁分泌因子促进卵母细胞核成熟,且这些因子的作用可能没有种属特异性.     

Chemical composition and hepatoprotective effects of polyphenol-rich extract from Houttuynia cordata tea

This study was designed to investigate the antioxidant activity, hepatoprotective effect, and phenolic composition of the ethyl acetate fraction (EAF) extracted from Houttuynia cordata tea. EAF was shown to exhibit strong ferric-reducing antioxidant power (FRAP) and scavenging activity against DPPH radical in vitro, and the antioxidant effects were further verified by suppressing CCl?-induced oxidative stress in mouse liver at three tested doses of EAF (250, 500, and 1000 mg/kg bw). Pretreatment with EAF (1000 mg/kg bw) prior to CCl? administration significantly (p < 0.001) decreased the CCl?-elevated levels of serum AST, ALT, alkaline phosphatase, total bilirubin, and hepatic MDA in mice and prevented the increases in GSH, SOD, and CAT caused by CCl?. HPLC analysis revealed that three predominantly polyphenolic compounds present in EAF were quercitrin (111.7 μg/mg), quercetin (43.8 μg/mg), and hyperoside (29.1 μg/mg). These results combined with liver histopathology indicate that EAF possesses a significant protective effect against acute hepatotoxicity induced by CCl?, which may be due to the strong antioxidant activity of phenolic components.     

Antihypertensive Effect of Protein Hydrolysate from Azufrado Beans in Spontaneously Hypertensive Rats

The objective of this study was to evaluate the antihypertensive potential of common bean protein hydrolysate. Protein concentrates were obtained, followed by Alcalase enzymatic hydrolysis, and then ultrafiltrated (3,000 molecular weight cutoff); the lyophilized product was named BP3. The angiotensin converting enzyme (ACE) inhibitory activity was determined as IC₅₀ (3.68 ± 0.07 μg/mL). The antihypertensive effect was evaluated in spontaneously hypertensive rats (SHR) by two assays; Captopril ACE inhibitor was used as a reference compound and water as a control. A short‐term assay showed a maximum decrease in mean arterial pressure of –41 ± 5 mmHg in SHR, 3 h after oral administration of 500 mg of BP3/kg of body weight (bw). In a long‐term assay, a significant decrease in systolic blood pressure of –24 ± 5 mmHg was observed in SHR, after 45 days of oral administration of 500 mg of BP3/kg of bw/12 h. In both assays, BP3 treatment showed antihypertensive effect over SHR, similar to Captopril treatment. The sequences of the most abundant peptides present in BP3, determined by mass spectrometry, were identified as KFPWVK, GADFRKK, and PQSPCKRVNRHS. These peptides are reported for the first time in Azufrado Higuera common beans, and they are most likely responsible for the antihypertensive effect of BP3.