户县某镇散养犬肠道寄生虫种类及感染情况的调查

为了解户县某镇散养犬肠道寄生虫感染情况,采用饱和食盐水漂浮法等对该地区散养犬120份粪便样品进行了检测。结果发现8种犬肠道寄生虫,分别是犬弓首蛔虫、等孢球虫、犬钩口线虫、粪类圆线虫、犬贾第虫、毛尾线虫、犬复孔绦虫、细粒棘球绦虫,其感染率分别为65.83%、40.83%、35.00%、26.67%、9.17%、6.67%、2.50%和1.67%。寄生虫总感染率为100%。在这些感染犬中,混合感染2种肠道寄生虫的为44例,混合感染3种以上肠道寄生虫的为31例,其混合感染率为62.5%;犬弓首蛔虫、等孢球虫和犬钩口线虫为优势种。     

新疆阿拉尔市就诊犬肠道寄生虫感染情况调查

为了解阿拉尔市就诊犬肠道寄生虫感染情况,采用直接涂片法和饱和食盐水漂浮法等对该地区就诊犬138份粪便样品进行检测。在粪样结果中发现犬肠道寄生虫有4种,分别是犬弓首蛔虫、犬等孢球虫、犬钩虫和犬复孔绦虫,其感染率分别是33.33%、18.12%、8.69%和3.62%,寄生虫总感染率为63.77%。在感染肠道寄生虫的犬只中,有23例混合感染2种肠道寄生虫,有11例混合感染2种以上肠道寄生虫,其混合感染率为39.77%;犬弓首蛔虫和等孢球虫为优势虫种。     

扬州市流浪犬肠道寄生虫种类的调查

为了解扬州市流浪犬肠道寄生虫的感染情况,采取离心沉淀法和饱和食盐水漂浮法分别对62份犬粪便样品进行检查。结果显示:共发现4种犬肠道寄生虫,分别是犬弓首蛔虫、钩口线虫、类圆线虫和犬等孢球虫,其感染率分别为30.65%、8.06%、3.23%和11.29%,寄生虫总感染率为48.39%;犬弓首蛔虫为优势种。混合感染2种寄生虫2例,混合感染3种寄生虫1例,混合感染率为4.84%。     

宠物犬肠道寄生虫调查及其公共卫生意义分析

为了解宠物犬肠道寄生虫,尤其是人兽共患肠道寄生虫感染情况,采用甲醛-乙酸乙酯沉淀法、卢戈氏碘液染色法和饱和蔗糖溶液漂浮法对郑州市宠物犬404份粪便样品进行了调查。结果共发现10种肠道寄生虫,寄生虫总感染率为37.12%,其中球虫和贾第虫为优势虫种,感染率分别为18.32%和12.62%;7种为人兽共患虫种,贾第虫、隐孢子虫、钩虫、弓首蛔虫、狮弓蛔虫、粪类圆线虫和犬复孔绦虫感染率分别为12.62%、3.71%、3.96%、6.93%、0.20%、1.73%和0.50%;2～3种寄生虫混合感染率为9.90%。结果分析表明,寄生虫感染与样品采集地点和犬年龄统计学差异极显著(P0.01),与性别无统计学差异(P0.05)。宠物犬肠道寄生虫感染较为普遍,人兽共患寄生虫流行率较高,应加强对其监控和防治。     

福州地区犬肠道寄生虫感染情况调查

2009年3月至2010年10月,对福州地区所辖福州市区、福清市和闽侯县的犬肠道寄生虫感染情况进行调查。收集来自福州市区、福清市和闽侯县的动物医院、农村散养户和流浪的不同品种,不同年龄,不同性别的526只犬的粪便,用水洗沉淀法、饱和盐水漂浮法和硫酸锌离心浮聚法进行肠道寄生虫检查,福州地区犬肠道寄生虫种类调查结果如下:(1)调查发现福州地区犬感染的肠道寄生虫共6种,包括犬钩虫(Ancylostoma coninum)、犬弓首蛔虫(Toxocara canis)、犬泡状带绦虫(Taenia hydatigena)、犬复孔绦虫(Dipylidium conium)、犬等孢球虫(Isospora canis)和犬华支睾吸虫(Clonorchis sinensis)。(2)福州地区犬消化道寄生虫总感染率为59.32%。(3)各种犬肠道寄生虫感染率不同,犬弓首蛔虫感染率为53.80%,犬钩虫感染率为22.62%,犬复孔绦虫感染率为25.86%,犬泡状带绦虫感染率为47.91%,犬等孢球虫感染率为7.98%,华支睾吸虫的感染率为2.09%。(4)调查发现有121例同时感染两种肠道寄生虫,占肠道寄生虫总感染率的23.00%,同时感染三种肠道寄生虫的有98例,占总感染率的18.63%。(5)调查发现不同年龄段其寄生虫感染率差异明显,幼犬感染率要明显高于成年犬,不同样品采集地点由于饲养环境不同,犬肠道寄生虫感染率有明显差异。(6)本次调查未发现犬肠道寄生虫感染在品种、性别方面的差异。     

农村散养犬肠道寄生虫种类及感染情况的调查

为了解兰考县某镇散养犬肠道寄生虫感染情况,采用饱和食盐水漂浮法等对该地区散养犬120份粪便样品进行了检测.结果发现8种犬肠道寄生虫,分别是犬弓首蛔虫、等孢球虫、犬钩口线虫、粪类圆线虫、犬贾第虫、毛尾线虫、犬复孔绦虫、细粒棘球绦虫,其感染率分别为65.83％、40.83％、35.00％、26.67％、9.17％、6.67％、2.50％和1.67％.寄生虫总感染率为100％.在这些感染犬中,混合感染2种肠道寄生虫的为44例,混合感染3种以上肠道寄生虫的为31例,其混合感染率为62.5％;犬弓首蛔虫、等孢球虫和犬钩口线虫为优势种.     

不同样品中犬输血传播性病毒的PCR检测比较与基因分析

根据NCBI上输血传播性病毒(Torque-Teno virus,TTV)犬基因组序列(Cf-TTV10,GenBank登录号:AB076002)设计嵌套引物,从犬血清与粪便中鉴定出TTV。199份犬血清样品中检出阳性率为14.6%(29/199),158份犬粪便样品的检出率为12.7%(20/158),通过基因分析与鉴定确定为同一型,验证了从粪便样品和血液样品的提取检测的犬TTV阳性率基本保持一致(P>0.05),为下一步进行TTV的传染特性和分子流行病学的研究提供了基础。     

新疆部分城市宠物犬肠道寄生虫感染情况调查

为了解新疆城市宠物犬肠道寄生虫感染情况,笔者采用饱和蔗糖溶液漂浮法对新疆5个城市的477份宠物犬粪便样本进行了检查。结果显示,113份样品呈肠道寄生虫虫卵/卵囊阳性,总感染率为23.7%。共检出7种(类)寄生虫虫卵或卵囊,以球虫为优势感染虫种,感染率为13.8%;蛔虫感染率次之,为9.2%;隐孢子虫,圆线虫,钩虫,鞭虫及带科绦虫感染率分别为0.6%、2.7%、0.4%、0.2%和0.2%。在宠物店内饲养犬的肠道寄生虫感染率较高,为33.2%;宠物医院及犬舍内宠物犬的肠道寄生虫感染率分别为14.7%和8.5%。雄犬感染率(26.0%)高于雌犬感染率(21.7%);6月龄以下,7～12月龄,1岁以上宠物犬的感染率分别为30.9%、20.5%和3.8%,随年龄增长呈递减趋势。调查结果表明,应进一步加强宠物犬肠道寄生虫的驱除工作和公共卫生知识宣传。     

沈阳某警犬基地犬肠道寄生虫感染调查

为了解沈阳某警犬基地犬肠道寄生虫感染情况,以及不同品种警犬、不同年龄段警犬间寄生虫感染存在的差异,采用直接涂片法和饱和盐水漂浮法对沈阳某警犬基地180只犬进行肠道寄生虫调查。结果表明:144只犬感染虫卵,感染率达到80%;检出虫卵种类有犬弓首蛔虫、狮弓蛔虫、犬复孔绦虫、细粒棘球绦虫等4种,感染率分别为36.67%,15.56%,18.89%和8.89%,其中犬弓首蛔虫和犬复孔绦虫为感染率较高的两种寄生虫;1～3月龄的幼犬与其他年龄犬相比,犬弓蛔虫感染率高达76.67%;只有德牧和德荷杂犬感染细粒棘球绦虫。     

477例门诊犬寄生虫种类和感染情况的调查

对2012年-2015年西北农林科技大学西安动物医院门诊确诊的477例犬寄生虫感染资料进行分类统计和分析,以期了解西安地区犬寄生虫的种类和感染情况,对犬寄生虫的防控提供依据。结果显示,从感染犬中共检出14种寄生虫,每种寄生虫的阳性率依次为:吉氏巴贝斯虫51.79%(247/477),等孢球虫16.15%(77/477),贾第鞭毛虫11.74%(56/477),犬弓首蛔虫6.71%(32/477),疥螨4.41%(21/477),蠕形螨4.41%(21/477),犬复孔绦虫3.78%(18/477),犬钩口线虫2.31%(11/477),犬耳痒螨1.89%(9/477),吸吮线虫0.63%(3/477),华支睾吸虫0.42%(2/477),弓形虫0.42%(2/477),犬尾旋线虫0.21%(1/477),犬恶丝虫0.21%(1/477)。混合感染两种及以上寄生虫的犬占5.45%(26/477)。肠道寄生虫中的等孢球虫、贾地鞭毛虫、犬弓首蛔虫和犬钩口线虫等容易发生混合感染。寄生虫和病毒合并感染犬占8.39%(40/477),犬细小病毒(CPV)、犬瘟热病毒(CDV)和犬冠状病毒(CCV)常与犬肠道寄生虫合并感染。许多寄生虫感染与犬的年龄有密切关系,季节对寄生虫感染也有影响。原虫感染特别是吉氏巴贝斯虫感染应当引起重视。控制犬与寄生虫传播媒介的接触、改善犬的饲养管理条件对寄生虫感染的预防十分重要。     

Heartworm in dogs in Canada in 1983

In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario.

Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously.

     

Heartworm in dogs in Canada in 1985

In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).

Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.

     

Heartworm in dogs in Canada in 1988

In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%).     

Heartworm in dogs in Canada in 1984

In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.

Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.

     

Heartworm in dogs in Canada in 1991

In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium.     

Heartworm in dogs in Canada in 1989

In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.

Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.

     

Heartworm in dogs in Canada in 1987

In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%).     

Heartworm in dogs in Canada in 1986

In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%).

Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada.