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对新疆地区奶牛乳房炎(包括隐性乳房炎和临床乳房炎)进行了发病率和致病菌进行了流行病学调查。结果发现新疆地区7个牧场隐性乳房炎发病率在11.3%~28.8%。大部分乳房炎发病为单一细菌感染引起。隐性乳房炎中共分离得到9个不同种属的病原菌,以凝固酶阴性葡萄球菌、乳房链球菌和无乳链球菌为最常见。临床乳房炎样品中共分离得到11个不同种属的样品,流行度最高的四种病原菌为凝固酶阴性葡萄球菌、乳房链球菌、大肠杆菌和肺炎克雷伯菌。在隐性乳房炎和临床乳房炎样品中均分离得到了金黄色葡萄球菌。 相似文献
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调查了合肥郊区部分奶牛场乳房炎发生情况,采用SMT检测法检测该奶牛场的隐性乳房炎,对100头奶牛进行检测,检出69头.隐性乳房炎阳性率为69%,并对乳样进行细菌分离鉴定,选择主要病原菌做药敏试验。结果表明,隐性乳房炎的主要致病菌为葡萄球菌、链球菌和大肠杆菌,它们对头孢噻吩钠、诺氟沙星、氨苄西林、羧苄青霉素等药物均高度敏感。 相似文献
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导致奶牛隐性乳房炎的因素很多,但病原微生物是导致隐性乳房炎的主要病因[1],其中以金黄色葡萄球菌、链球菌和大肠杆菌为主,但各地病原感染情况不尽相同。本试验通过对柳州市部分奶牛场采集奶样进行细菌的分离及药敏试验,为奶牛隐性乳房炎的防治、用药提供可靠依据,以促进柳州市奶牛养殖业的发展。 相似文献
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张连成 《北方牧业(奶牛)》2009,(8):55-56
我国奶牛乳房炎的发病率很高,隐性乳房炎的发病率更高、危害性更大。根据国际奶牛联合会的统计,20世纪70年代,奶牛乳房炎的发病率是2%,隐性乳房炎患病率为50%。在我国,同期几个大城市隐性乳房炎的检出率为20%-70%。20世纪80年代,有60%以上的乳牛都感染有隐性乳房炎,有30%-50%的乳区感染有隐性乳房炎。近年来奶牛隐性乳房炎呈上升趋势。 相似文献
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1什么是奶牛隐性乳房炎奶牛隐性乳房炎是一种发病率高,没有临床症状,也就是奶水是白色乳状,乳房组织有肿胀和痛疼,但是乳汁的生物化学、乳汁成份则发生变化,肉眼看不到乳汁和乳房变化,所以叫做隐性乳房炎。隐性乳房炎有什么危害呢?最大的危害是隐性乳房炎能由于病情发展,变成显性乳房炎,乳汁出现絮状物(豆腐脑样子);乳房组织肿胀,痛疼,甚至病牛发烧,不出奶。其次是奶牛得了隐性乳房炎产奶量下降,一般要减少乳量10%左右。奶牛隐性乳房炎是怎样得的呢?隐性乳房炎由于现在检查方法有了,人们都开始重视这方面的问题,关于… 相似文献
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MA Chun-xia LI Jun QIN Yong ZHONG Shu-hong TAO Li PENG Hao LAN Mei-yi YANG Wei CHEN Ze-xiang HU Shuai LI Chang-ting ZHAO Xiao-hong 《中国畜牧兽医》2015,42(9):2481-2486
To investigate the pathogen of bovine respiratory disease complex (BRDC) of a dairy farm in Guangxi, a strain of Mycoplasma and a strain of gram-negative pathogenic bacterium were isolated and identified by the means of field surveys, clinic observation, pathological examination, isolation studies and so on.Treatments were taken according to drug sensitivity test results.The Mycoplasma strain, growing on PPLO medium, formed typical "fried egg" colonies.A 448 bp of oppF fragment was amplified by PCR from the strain and had 98.4% nucleotide identity with Mycoplasma bovis reference isolate PG5 of USA.The biochemical features of the gram-negative bacterial isolate were same with Serratia marcescens.The PCR amplified 16S rDNA of the gram-negative pathogenic bacterium strain was 1 400 bp.It shared 99.0% nucleotide identity with other Serratia marcescens reference strains obtained from GenBank.Animal experiment showed that the gram-negative pathogenic bacterium isolate could cause the mice to die.The drug sensitivity tests showed all isolates were sensitive to spectinomycin, azithromycin, amikacin, gentamicin and neomycin.It was effective to treat with dexamethasone and spectinomycin.Pathogen analysis and drug treatment showed that the BRDC was caused by Mycoplasma bovis and Serratia marcescens. 相似文献
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牛呼吸道疾病综合征病例的病原分析 总被引:1,自引:0,他引:1
本试验旨在查清广西某牛场1起牛呼吸道疾病综合征(BRDC)病例的病原,指导牛场进行疾病防控。采取现场调查、临床症状与病理变化、病原分离鉴定等方法对病例病原进行分析,根据病原药敏试验结果进行治疗。从病例的肺脏组织中分离到1株支原体和1株革兰氏阴性致病杆菌。支原体分离株在PPLO固体培养基上可见典型的"煎蛋样"菌落,PCR扩增出牛支原体oppF基因特异性的448 bp目的片段,其oppF基因序列与美国分离的牛支原体国际标准株PG45的核苷酸序列同源性为98.4%。革兰氏阴性细菌分离株生化特性符合黏质沙雷氏菌特性,其16S rRNA基因PCR扩增出1 400 bp的目的片段,测序结果与GenBank上登录的黏质沙雷氏菌的核苷酸序列同源性达到99.0%,对小鼠具有致病性。牛支原体和黏质沙雷氏菌分离株均对壮观霉素、阿奇霉素、阿米卡星、庆大霉素和新霉素高度敏感,用高敏药物壮观霉素联合地塞米松等相关措施进行治疗,收到良好效果。结果表明,引起这次牛呼吸道疾病综合征的病原为牛支原体和黏质沙雷氏菌。 相似文献
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从患病死亡的旧院黑鸡病料中分离纯化得到一株细菌,经染色镜检、生化试验和动物致病性试验,最终鉴定为致病性大肠杆菌。选用临床常用的12种抗生素对该菌株进行了药敏试验.结果显示该菌对庆大霉素、阿米卡星、氟苯尼考敏感;对氨苄西林/克拉维酸、卡那霉素、环丙沙星中敏;对氨苄西林、链霉素等耐药。 相似文献
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Sasaki Y Yamamoto K Kojima A Norimatsu M Tamura Y 《Research in veterinary science》2000,69(3):289-294
In cattle, sheep, and other ruminants, clostridial myonecrosis (gas gangrene) is mostly caused by Clostridium chauvoei, C septicum, C novyi and C sordellii. A polymerase chain reaction (PCR) system using common primers designed from multiple alignment of the 16S rRNA and 23S rRNA genes of Clostridium species was developed to identify pathogenic clostridia. The PCR was performed with total DNA from 26 strains which included seven different Clostridia species. These bacteria were differentiated at species level by the different PCR product patterns. To characterise the 16S-23S rDNA spacer regions of these clostridia further, most PCR products of these bacteria were sequenced. The smallest PCR products of each bacterium represented the fundamental 16S-23S rDNA spacer region; larger PCR products of each bacterium were caused by insertion sequences, i.e. tRNA gene sequences. The authors' observations indicate that the PCR patterns of the 16S-23S rDNA spacer regions have the potential to be used as an identification marker of pathogenic clostridia in gas gangrene. 相似文献
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对广东省佛山市某乌鳢养殖鱼场患病的乌鳢进行病原分离,将疑似分离菌S-1株进行形态和培养特性、生化特性鉴定、PCR鉴定、药敏试验和人工感染等试验。结果表明,该分离菌为舒氏气单胞菌。药敏试验结果表明,本分离菌对壮观霉素、米诺环素、庆大霉素、氧氟沙星、诺氟沙星、头孢类等多种药物敏感。本研究为舒氏气单胞菌病细菌学检测及治疗提供了科学依据。 相似文献
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Staphylococcus hyicus in cattle 总被引:13,自引:0,他引:13
Staphylococcus hyicus (subsp hyicus) was found to be a frequently occurring inhabitant of the skin of cattle. Significant numbers of this bacterium were found to be present in mange lesions of cattle between one and three years old. Experimental inoculations revealed that S hyicus had a pathogenic effect on the superficially scarified skin of young cattle. 相似文献
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用已建立的四重荧光PCR方法、传统国标或行标方法、MALDI Biotyper高通量微生物鉴定法(基质辅助激光解析电离飞行时间质谱法)对随机抽取的14份食品样品进行检测,比较3种检测方法的匹配性。并用四重荧光PCR对农贸市场、大型超市采集的10类食品共205份样品进行检测。旨在研究四重荧光PCR法在食品中检测沙门氏菌、弗氏枸橼酸杆菌、奇异变形杆菌和迟缓爱德华菌的应用及食品中这4种菌的污染情况。在205份食品样品中,受污染的食品有110份,污染率53.66%(110/205),同时检出两种及两种以上致病菌的样品有62份,占污染样品的56.36%(62/110)。迟缓爱德华菌大肠菌、弗氏枸橼酸杆菌、奇异变形杆菌、沙门氏菌的检出率分别为7.80%(16/205)、53.66%(110/205)、26.34%(54/205)、5.85%(12/205)。在这3种检测方法中,四重荧光PCR的特异性好、准确性高,操作简便省时,更适用于食品中4种致病菌的快速检测。抽检的食品样品中存在这4种菌的污染,尤以生禽畜肉和水产品污染严重,需加强对食品加工、流通环节的卫生监管和监测。 相似文献