哈茨木霉对黄瓜尖孢镰刀菌的抑制作用和抗性相关基因表达

采用室内筛选与田间防效相结合的方法,对哈茨木霉抑制黄瓜尖孢镰刀菌的拮抗机制进行了研究。对峙培养结果显示,木霉菌和病原菌间均形成了较明显的抑菌圈,对病原菌的抑菌率达66.7%~85.8%,其中菌株TG、TM对枯萎病菌抑制作用较强。木霉菌可有效提高根系对病原菌的抗性,黄瓜植株接种枯萎病菌后,根系细胞大量死亡,而先接种木霉菌再接种病原菌后则减小了对根系的伤害。田间防效试验结果表明,TG和TM孢子悬浮液浓度在108个/mL时防效最好,分别为54.9%和49.4%。接种木霉菌植株根系抗性基因的表达量均高于对照植株,呈双峰趋势。在第6天时抗性基因表达量最高,WRKY6、MYB、PR-1、PAL、GST和GLU的表达量分别为对照的5.15、5.22、6.07、6.00、3.16、和16.15倍。表明木霉菌通过激活与胁迫相关的基因表达提高了对病原菌的抗性。     

桑树青枯病与根际土壤肥力及微生物群落结构特征的研究

本文比较分析了桑树青枯病发病和健康植株根际土壤肥力以及微生物（细菌和真菌）群落结构特征差异，旨在挖掘和利用丰富微生物资源的有益功能，为构建桑树青枯病生物防治技术体系奠定基础。结果表明，桑树青枯病发病植株根际土壤中指示土壤肥力与健康状况的生物学性状指标β-葡糖苷酶和磷酸酶的活性显著降低，微生物生物量碳、磷显著下降；细菌门分类水平上，发病植株根际土壤中酸杆菌门、放线菌门和疣微菌门等优势细菌相对丰度降低，变形菌门、绿弯菌门、粘球菌门、厚壁菌门、拟杆菌门等优势细菌相对丰度增加；细菌属分类水平上，发病植株根际土壤富集了硝化螺菌属和放线菌属等特有细菌属，缺失了诸如链霉菌属等具有分泌抗生素功能的优势细菌属。真菌门分类水平上，发病植株根际土壤中子囊菌门和担子菌门真菌相对丰度增加，unclassified_k_Fungi和霉菌门真菌相对丰度降低；真菌属分类水平上，发病植株根际土壤富集了地霉菌属、腐质霉属和丝孢菌属等腐生真菌，缺失了被孢霉属、枝顶孢属和曲霉属等具有产抑菌化合物功能的优势真菌。研究表明，桑树青枯病与根际土壤β-葡糖苷酶、磷酸酶活性，微生物生物量碳、磷以及微生物群落结构都具有一定的相关性。链霉菌属细菌以及被孢霉属、枝顶孢属和曲霉属真菌有望作为生物防控青枯病的有效备选菌属。     

拟康宁木霉Hailin菌株对马尾松幼苗的促生和防病作用

采用菌株形态学和r DNA-ITS序列比对分析的方法进行了Hailin菌株的鉴定,采用平皿对峙法研究了Hailin菌株对尖孢镰孢菌(Fusarium oxysporum)的抑菌效果,测定了接种尖孢镰孢菌和Hailin菌株后对马尾松(Pinus massoniana Lamb)幼苗的发病率和生长指标的影响。经形态学、分子鉴定和进化树分析,菌株Hailin为拟康宁木霉(Trichoderma koningiopsis)。Hailin菌株与尖孢镰刀菌平皿对峙培养5d后,对其抑制率为77.78%;蘸根针刺微伤处理接种Hailin菌株30 d后,对马尾松幼苗立枯病的防治率为58.3%;接种Hailin菌株与不接种的马尾松幼苗相比,其株高、湿重、根长分别提高了3.86%、78.74%和13.2%。Hailin菌株抑制尖孢镰孢菌的作用机制包括竞争作用、重寄生作用以及通过产生代谢产物抑制病原菌的生长。其中,Hailin菌株通过非挥发性代谢产物和挥发性代谢产物对尖孢镰孢菌菌丝生长的抑制率分别为97.80%和77.80%。     

复合微生态制剂对黄瓜根际土壤微生物数量和酶活性的影响

[目的]研究施入复合微生态制剂对黄瓜根际土壤的影响.[方法]采用盆栽试验的方法,对不同生育时期施入复合微生态制剂的黄瓜根际土壤微生物数量及土壤酶活性进行了测定.[结果]与对照相比,在花期施入复合微生态制剂,可使根际土壤细菌和放线菌的数量分别增加27.9％和20.6％,而真菌和尖孢镰刀菌的数量分别减少64.5％和66.7％;可使土壤脲酶、蔗糖酶、中性磷酸酶活性分别提高130.8％、190.5％、123.5％,差异均显著.黄瓜不同生育时期施入复合微生态制剂对过氧化氢酶作用不明显.[结论]本研究在花期施入复合微生态制剂后,在一定程度上,可提高黄瓜根际土壤细菌和放线菌的数量,减少真菌及尖孢镰刀菌的数量,提高土壤脲酶、蔗糖酶和中性磷酸酶的活性.     

微生物菌剂处理增加了生姜土壤中有益微生物的相对丰度

作物连作易导致土壤中病原菌大量积累, 促使土传病害频发, 影响作物生长?本试验采用微生物菌剂(富含哈茨木霉Trichoderma harzianum TH7?淡紫拟青霉Purpureocillium lilacinum PL22以及高效枯草芽胞杆菌Bacillus subtilis)处理具有多年生姜种植历史的地块, 并采用分离培养法和宏基因组测序技术对处理后土壤中的微生物进行检测, 明确微生物菌剂对生姜土壤中微生物群落组成的影响?结果表明, 与对照相比, 微生物菌剂处理后, 土壤中细菌属和种的数量增加, 而真菌属和种的数量减少?从微生物菌剂处理后的土壤中分离出3个门水平, 24个属水平和42个种水平细菌, 以及3个门水平, 20个属水平和24个种水平真菌, 其中分离频率较高的细菌和真菌种分别为温哥华假单胞菌Pseudomonas vancouverensis和Musidium stromaticum?从未经微生物菌剂处理的土壤(对照)中分离出导致生姜茎基腐病的病原菌尖镰孢Fusarium oxysporum和腐皮镰孢 F. solani?宏基因组测序结果表明, 微生物菌剂处理对真菌多样性的影响大于对细菌?与对照相比, 微生物菌剂处理提高了土壤中根瘤菌属Rhizobium?假单胞菌属Pseudomonas?芽胞杆菌属Bacillus等细菌属及Musidium?被孢霉属Mortierella等真菌属物种的相对丰度?综上, 微生物菌剂处理增加了生姜土壤中细菌属和种的数量, 增加了土壤中与有益微生物相关的细菌和真菌的相对丰度, 并且抑制了病原菌的生长?     

微生物菌剂对连作芹菜根际土壤真菌群落多样性与结构的影响

以宁南旱区连作4年芹菜根际土壤为研究对象,采用田间定位试验结合高通量测序技术,以常规施肥（CK）为对照,研究常规施肥＋枯草·哈茨复合菌剂（MF）,常规施肥＋枯草芽孢杆菌（BS）,常规施肥＋哈茨木霉菌（TH）3个处理对芹菜连作根际土壤真菌多样性及群落组成的影响。结果表明：施用枯草·哈茨复合菌显著提高了土壤有效磷、速效钾含量,增幅分别为34.29%和9.98%。施用微生物菌剂增加了土壤脲酶、蔗糖酶活性,平均增幅分别为43.16%和12.98%,并显著改变了土壤真菌的β-多样性。被孢霉属(Mortierella)、织球壳属(Plectosphaerella)、赤霉菌属(Gibberella)、链格孢属(Alternaria)、镰刀菌属(Fusarium)是各处理优势菌属,施用枯草·哈茨复合菌显著降低了赤霉菌属、链格孢属、镰刀霉属等真菌性病原菌的相对丰度,降幅分别为59.88%、51.63%、36.13%。冗余分析表明土壤蔗糖酶、脲酶、碱性磷酸酶活性、pH值和全磷是影响真菌群落结构组成的主要驱动因子。综合而言,枯草·哈茨复合菌剂的施用不仅降低了连作芹菜根际土壤镰刀菌属、链格孢属、赤霉菌属等病原菌的富集,还通过改变有效磷、速效钾等土壤理化性质,进一步重塑土壤真菌群落结构。     

根皮苷对苹果再植病害及对再植土壤真菌群落的影响

为研究根皮苷对诱发苹果再植病害的作用和影响机制，本文通过盆栽实验研究了不同浓度根皮苷对八棱海棠幼苗生长和土壤酶活性，对病原真菌菌丝生长和产孢以及对土壤真菌群落结构的影响。结果表明，添加外源根皮苷显著限制了盆栽八棱海棠幼苗的生长，其中对株高影响最大，降低了65.3%;改变了中性磷酸酶、脲酶、多酚氧化酶和过氧化氢酶等再植土壤酶活性；刺激腐皮镰孢菌菌丝的生长，显著增加了层出镰孢菌的菌丝干重与产孢量，分别提高48.2%和60.0%。重要的是，添加外源根皮苷的土壤和原始土壤及对照土壤相比，真菌群落组成和结构发生了很大变化。高通量测序结果分析表明，添加40 mg·kg^-1根皮苷显著降低了土壤真菌群落的多样性和均匀度，其中添加根皮苷的非灭菌土壤中与再植病害发生程度具有负相关性的被孢霉属的相对丰度显著降低。因此，根皮苷的积累可以改变真菌群落结构和土壤酶活性，并在一定程度上促进病原镰孢菌的生长和产孢，抑制再植苹果幼树的生长，进而诱导苹果再植病害的发生。     

厚朴苗根腐病的病原菌鉴定

 经病原菌分离培养、致病性测定、形态特征观察及专化型试验结果表明,厚朴苗根腐病由尖镰孢霉内的一个新的专化型(Fusarium oxysporum Schlecht f. sp. magnoliae)为害所致。     

山东省小麦根腐病病原菌的分离鉴定

为明确山东省小麦根腐病的病原菌种类,于2012—2014年从山东省10个地市采集小麦病株,通过组织分离法获得了185株分离物,利用形态学鉴定方法,结合基于5.8S r DNA-ITS序列或TEF-1α基因序列分析的分子鉴定方法对分离物进行了鉴定。结果表明:分离物中共得到135株麦根腐平脐蠕孢Bipolaris sorokiniana,占分离病原菌总数的72.97%,属优势种群;50株镰孢属Fusarium菌株,其中14株尖孢镰孢菌Fusarium oxysporum、19株层出镰孢菌Fusarium proliferatum和17株黄色镰孢菌Fusarium culmorum;按照柯赫氏法则进行致病性测定,证实了4种病原菌对鲁麦21号具有致病性,麦根腐平脐蠕孢的致病力较强,病情指数显著高于镰孢菌属真菌。研究表明,山东小麦根腐病主要是由麦根腐平脐蠕孢和镰孢属真菌侵染引起的,麦根腐平脐蠕孢为优势菌群。     

一株拮抗4种马铃薯病原真菌的拟康宁木霉的分离鉴定

本研究从黑龙江省土壤中分离获得一株木霉, 运用生物学特征和ITS序列分析相结合的方法将其鉴定为拟康宁木霉Trichoderma koningiopsis?薯块活体抑菌测定表明, 该木霉对接骨木镰孢Fusarium sambucinum?马铃薯早疫病菌Alternaria solani?立枯丝核菌Rhizoctonia solani (AG3和AG5)等病原真菌引起的马铃薯病害具有抑制作用?对峙培养试验结果显示, 该木霉对上述病原菌的生长具有拮抗作用, 平均抑制率均超过50%, 且该木霉生长速度快于上述病原真菌, 以竞争生长方式抑制病原菌菌丝生长和菌核形成?采用扫描电子显微镜观察发现, 该木霉可通过缠绕或并行生长等方式寄生在上述病原真菌菌丝上?以上结果表明, 从土壤中分离获得的拟康宁木霉具备良好的生防潜力, 可为马铃薯种植中多种真菌病害的统防统治提供良好的生防菌株?     

Dermestid beetles in ‘evolution Canyon’, lower Nahal Oren, Mt. Carmel, including new records for Israel

Fifteen species of dermestid beetles were recorded at ‘Evolution Canyon’ (EC), Lower Nahal Oren, Mt. Carmel, Israel. They represent ~35% of known Israeli dermestid species. The following three species were recorded for the first time in Israel:Trogoderma svriaca Dalla Torre, 1911;Ctesias svriaca Ganglbauer, 1904; andAnthrenus (s.str.) jordaniens Pic, 1934. Adults of 13 species were collected on the more solar radiated, warmer and climatically more fluctuating south-facing slope (SFS); ten species were collected on the opposite, north-facing slope (NFS), which was cooler and climatically more stable. The abundance of adult dermestid beetles was 1.9 times higher on the SFS than on the NFS (86 and 47, respectively). Species richness and abundance distribution at EC (three collecting stations on each slope and one at the valley bottom) were significantly negatively correlated with the plant cover that consisted of trees and bushes (Spearmanr _s ,P=0.007 and 0.039, respectively) and perennials (Spearmanr _s ,P=0.039 and 0.077, respectively), indicating that non-woody plants were preferred by adult dermestid beetles.     

Hymenopteran Parasitoids on Fruit-infesting Tephritidae (Diptera) in Latin America and the Southern United States: Diversity, Distribution, Taxonomic Status and their use in Fruit Fly Biological Control

We first discuss the diversity of fruit fly (Diptera: Tephritidae) parasitoids (Hymenoptera) of the Neotropics. Even though the emphasis is on Anastrepha parasitoids, we also review all the information available on parasitoids attacking flies in the genera Ceratitis, Rhagoletis, Rhagoletotrypeta, Toxotrypana and Zonosemata. We center our analysis in parasitoid guilds, parasitoid assemblage size and fly host profiles. We also discuss distribution patterns and the taxonomic status of all known Anastrepha parasitoids. We follow by providing a historical overview of biological control of pestiferous tephritids in Latin American and Florida (U.S.A.) and by analyzing the success or failure of classical and augmentative biological control programs implemented to date in these regions. We also discuss the lack of success of introductions of exotic fruit fly parasitoids in various Latin American countries. We finish by discussing the most pressing needs related to fruit fly biological control (classical, augmentative, and conservation modalities) in areas of the Neotropics where fruit fly populations severely restrict the development of commercial fruit growing. We also address the need for much more intensive research on the bioecology of native fruit fly parasitoids.     

Diagnostics,genetic diversity and pathogenic variation of ascochyta blight of cool season food and feed legumes

Molecular diagnostic techniques have been developed to differentiate the Ascochyta pathogens that infect cool season food and feed legumes, as well as to improve the sensitivity of detecting latent infection in plant tissues. A seed sampling technique was developed to detect a 1% level of infection by Ascochyta rabiei in commercial chickpea seed. The Ascochyta pathogens were shown to be genetically diverse in countries where the pathogen and host have coexisted for a long time. However, where the pathogen was recently introduced, such as A. rabiei to Australia, the level of diversity remained relatively low, even as the pathogen spread to all chickpea-growing areas. Pathogenic variability of A. rabiei and Ascochyta pinodes pathogens in chickpea and field pea respectively, appears to be quantitative, where measures of disease severity were based on aggressiveness (quantitative level of infection) rather than on true qualitative virulence. In contrast, qualitative differences in pathogenicity in lentil and faba bean genotypes indicated the existence of pathotypes of Ascochyta lentis and Ascochyta fabae. Therefore, reports of pathotype discrimination based on quantitative differences in pathogenicity in a set of specific genotypes is questionable for several of the ascochyta-legume pathosystems such as A. rabiei and A. pinodes. This is not surprising since host resistance to these pathogens has been reported to be mainly quantitative, making it difficult for the pathogen to overcome specific resistance genes and form pathotypes. For robust pathogenicity assessment, there needs to be consistency in selection of differential host genotypes, screening conditions and disease evaluation techniques for each of the Ascochyta sp. in legume-growing countries throughout the world. Nevertheless, knowledge of pathotype diversity and aggressiveness within populations is important in the selection of resistant genotypes.     

Epidemiology of Toxigenic Fungi and their Associated Mycotoxins for Some Mediterranean Crops

Recent data on the epidemiology of the common mycotoxigenic species of Fusarium, Alternaria, Aspergillus and Penicillium in infected or colonized plants, and in stored or processed plant products from the Mediterranean area are reviewed. Emphasis is placed on the toxigenicity of the causal fungal species and the natural occurrence of well known mycotoxins (aflatoxins, ochratoxins, fumonisins, trichothecenes, zearalenone, patulin, Alternaria-toxins and moniliformin), as well as some more recently described compounds (fusaproliferin, beauvericin) whose toxigenic potential is not yet well understood. Several Fusarium species reported from throughout the Mediterranean area are responsible of the formation of mycotoxins in infected plants and in plant products, including: Fusarium graminearum, F. culmorum, F. cerealis, F. avenaceum, F. sporotrichioides and F. poae, which produce deoxynivalenol, nivalenol, fusarenone, zearalenone, moniliformin, and T-2 toxin derivatives in wheat and other small grains affected by head blight or scab, and in maize affected by red ear rot. Moreover, strains of F. verticillioides, F. proliferatum, and F. subglutinans, that form fumonisins, beauvericin, fusaproliferin, and moniliformin, are commonly associated with maize affected by ear rot. Fumonisins, were also associated with Fusarium crown and root rot of asparagus and Fusarium endosepsis of figs, caused primarily by F. proliferatum. Toxigenic A. alternata strains and associated tenuazonic acid and alternariols were commonly found in black mould of tomato, black rot of olive and citrus, black point of small cereals, and black mould of several vegetables. Toxigenic strains of A. carbonarius and ochratoxin A were often found associated with black rot of grapes, whereas toxigenic strains of A. flavus and/or P. verrucosum, forming aflatoxins and ochratoxin A, respectively, were found in moulded plant products from small cereals, peanuts, figs, pea, oilseed rape, sunflower seeds, sesame seeds, pistachios, and almonds. Finally, toxigenic strains of P. expansum and patulin were frequently found in apple, pear and other fresh fruits affected by blue mould rot, as well as in derived juices and jams.     

Brief Guide for Authors

正Journal of Arid Land(JAL)is an international journal(ISSN 1674-6767;CN 65-1278/K)for the natural sciences,sponsored by the Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences and Science Press.It is published by Science Press and Springer-Verlag Berlin Heidelberg bimonthly.JAL publishes original,innovative,and integrative research from arid and semiarid regions,ad     

A survey of the genera of<Emphasis Type="Italic">Microgaster</Emphasis> latreille and<Emphasis Type="Italic">Hygroplitis</Emphasis> thomson (Hymenoptera: Braconidae: Microgastrinae) from China

The genera ofMicrogaster Latreille 1804 andHygroplitis Thomson 1895 from China are presented systematically in this paper. Thirty-two species ofMicrogaster and three species ofHygroplitis are known in China. Diagnosis, character variation, distribution and host of each species among the two genera are presented, including its host and distribution. Keys to the species ofMicrogaster andHygroplitis are given. http://www.phytoparasitica.org posting Dec. 19, 2006.     

Plant Viruses Transmitted by Whiteflies

One-hundred and fourteen virus species are transmitted by whiteflies (family Aleyrodidae). Bemisia tabaci transmits 111 of these species while Trialeurodes vaporariorum and T. abutilonia transmit three species each. B. tabaci and T. vaporariorum are present in the European–Mediterranean region, though the former is restricted in its distribution. Of the whitefly-transmitted virus species, 90% belong to the Begomovirus genus, 6% to the Crinivirus genus and the remaining 4% are in the Closterovirus, Ipomovirus or Carlavirus genera. Other named, whitefly-transmitted viruses that have not yet been ranked as species are also documented. The names, abbreviations and synonyms of the whitefly-transmitted viruses are presented in tabulated form together with details of their whitefly vectors, natural hosts and distribution. Entries are also annotated with references. Whitefly-transmitted viruses affecting plants in the European–Mediterranean region have been highlighted in the text.     

The parasitoid complex ofLiriomyza cicerina on chickpea (Cicer arietinum)

Liriomyza cicerina (Diptera: Agromyzidae) is an important pest on chickpea in Turkey. The objective of this study was to determine the parasitoids and rates of parasitism ofL. cicerina on chickpea (Cicer arietinum L.) during the 2005 and 2006 seasons in ?anl?urfa province, Turkey. Leaves with mines were sampled weekly and kept in the laboratory to observe and count emerging leafminer and parasitoid adults. Eight parasitoid species were collected: the braconidsOpius monilicornis Fischer andOpius tersus Foerster and the eulophidsDiaulinopsis arenaria (Erdös) andNeochrysocharis formosa (Westwood), which occurred in both the winter and summer seasons;Diglyphus crassinervis Erdös,Neochrysocharis ambitiosa Hansson,Neochrysocharis sericea (Erdös) andPediobius metallicus (Nees), which occurred only in the summer growing areas.Diaulinopsis arenaria was the predominant parasitoid with 4–7.7% parasitism rate whileN. ambitiosa andO. monilicornis were the second and third most predominant species. The results of these trials show that sinceDia. arenaria occurred throughout every season, it could potentially be used for control of the leafminerL. cicerina.     

Plant–herbivore asynchrony necessitates augmentative releases of the exotic beetle,Zygogramma bicolorata,to enhance the biological control of P arthenium hysterophorus

Systematic information on the quantitative impact of Z ygogramma bicolorata on the biology of P arthenium hysterophorus is crucial as the seeds of this weed continue to germinate from the accumulated soil seed bank throughout the year in the form of different germinating flushes, while the activity of the beetle ceases during winter as it enters diapause. Therefore, plant–herbivore interactions need to be explored to develop predictions of the overall impact of the introduced beetle on the weed. The findings revealed that defoliation by Z . bicolorata had a significant impact on the plant height, density and flower production in flushes F ₃, F ₄ and F ₅, but not in F ₁ and F ₂ that exhibited longer periodicity, profuse branching, a longer flowering period and maximum flower production and contributed mostly to the existing seed soil bank. Therefore, total depletion of the existing soil seed bank was not possible. Consequently, the effect of augmentative field releases of laboratory‐reared beetles was explored on F ₁ and F ₂ in February for three consecutive years (2011–2013). Before initiating the trial, random soil samples were taken from the plots that were assigned to the paired treatments (i.e. with the beetle and without the beetle [insecticide‐treated]) and it was found that the seed bank in those samples did not differ. The single release of Z . bicolorata adults at five per plant at the six‐leaf stage significantly reduced the soil seed bank, compared to without the biocontrol agent, irrespective of the flushes at the end of the season.     

The use of Solacol® (validamycin) as a growth retardant in the isolation of soil fungi

Solacol^®, a formulation of the antibiotic validamycin, at 0.33% in 2% malt extract agar, reduced the spread of fungi on dilution plates drastically and allowed twice as much incubation time before subculturing; this resulted in an elevated number of species isolated. Using pure cultures of 62 common soil fungi, it was shown that all fast-growing species (exceptPythium ultimum) were efficiently inhibited but not completely suppressed. Inhibition was comparable to that by 0.5% oxgall, though, while this substance completely suppressed several species, Solacol very strongly inhibited onlyGaeumannomyces graminis, Gerlachia nivalis, Harzia acremonioides, Verticillium biguttatum andRhizoctonia solani. In a further experiment each separate constituent of Solacol was tested against 22 fungi at equivalent concentrations. Validamycin strongly inhibitedChaetomium globosum and two Basidiomycetes, though hardly more than the non-ionic detergent which mainly inhibited the other fungi. A few species were, however, more inhibited by Solacol than by the detergent alone. Solacol at 0.33% is a suitable aid in dilution plating of soil fungi, by increasing the number of colonies and species observed.Samenvatting Solacol^®, een formulering van het antibioticum validamycine, remde de groei van schimmels in verdunningsplaten met een concentratie van 0.33% in 2% moutagar en maakte het mogelijk de periode tot afenten met een factor 2 te verlengen; daardoor was het aantal geïsoleerde soorten duidelijk toegenomen. Met reincultures van 62 algemene grondschimmelsoorten werd aangetoond, dat alle snelgroeiende soorten (met uitzondering vanPythium ultimum) voldoende geremd, maar niet volkomen onderdrukt werden. Het remmingspercentage was vergelijkbaar met dat van 0.5% ossegal, hoewel dit laatste sommige soorten volkomen onderdrukte; Solacol remde alleenGaeumannomyces graminis, Gerlachia nivalis, Harzia acremonioides, Verticillium biguttatum enRhizoctonia solani zeer sterk. In een volgend experiment werden de componenten van Solacol t.o.v. 22 fungi apart getoetst in concentraties equivalent aan 0.33% Solacol. Validamycine remde alleenChaetomium globosum en twee basidiomyceten behoorlijk, maar nauwelijks meer dan de niet-ionische uitvloeier, die in hoofdzaak de overige remeffecten veroorzaakte. Enkele soorten werden echter door het complete Solacol veel sterker geremd dan door de uitvloeier alleen. Solacol in een verdunning van 0,33% wordt aanbevolen bij verdunningsplaten voor het isoleren van grondschimmels ten einde het aantal kolonies en soorten te verhogen.