Effect of sorbitol on moisture transportation and textural change of fish and squid meats during curing and drying processes

SUMMARY: Atka mackerel (Am) and Japanese common squid (Sq) meats were cured in 0.5–1.5 M sorbitol solutions (pH 7.0) and dried at 30°C (relative humidity, 60%), and the effect of sorbitol on the moisture transportation and textural change during the curing and drying processes was investigated. With an increase in sorbitol permeated through samples, the moisture contents decreased by 52% (Am) and 42% (Sq) by curing in 1.5 M sorbitol solution. When the cured meats were dried, slow moisture vaporization occurred at the initial drying period, and the critical moisture content significantly decreased with an increase in the sorbitol content of the cured meats. Further, the hardening of the dried products was effectively suppressed by sorbitol curing. These effects of sorbitol would contribute to the reduction of drying time and particularly the elimination of the excess hardening of dried fish products.     

Changes in meat texture of three varieties of squid in the early stage of cold storage

ABSTRACT: The present study examined the changes in texture and protein components during cold storage of different squid varieties. Raw oval squid, Japanese common squid and arrow squid were sliced fresh and the muscles were stored at 4°C for 0, 4, 8, 12, 18, 24, 48 and 120 h. The rheological measurements, protein components and amounts of collagen were examined. The adhesiveness of each squid increased significantly in the early stage of cold storage. In all varieties, penetration decreased at 4 h, which is considered to be rigor mortis, then increased. The amounts of total collagen, 20°C water-soluble collagen and 70°C water-soluble collagen did not change significantly in each variety during cold storage. Sodium dodecylsulfate–polyacrylamide gel electrophoresis (SDS-PAGE) pattern showed that the 580 kDa component gradually disappeared up to 48 h. The correlations between the amounts of 580 kDa component and adhesiveness or firmness were high. Models of fit based on chemical kinetics accurately expressed the behavior of adhesiveness, firmness and penetration showing that 63.2% of adhesiveness changes occurred in 13–19 h and that 63.2% of firmness changes occurred in 18–24 h.     

An Intermediate Moisture Product from Mackerel (Rastrelliger kanagurta) Using Salt Curing,Fermentation, and Drying

Intermediate moisture products were prepared from mackerel (Rastrelliger kanagurta) using salt curing in 20% or saturated brine for 20 hr and fermentation with an inoculum level of l0⁸ cells/mL of Pediococcus acidilactici followed by sun drying (28- 33C) for 23 hr or drying in an electric oven (45-50C) for 14 hr to an 18% moisture level. The chemical and microbiological studies correlated with the organoleptic results, suggesting a shelf-life of 4 months for salted and dried products and 7 months for salted, fermented, and dried products.     

Drying Kinetics and Quality Characteristics of Slightly Salted Grass Carp Fillets by Hot Air Drying and Vacuum Microwave Drying

Salted grass carp fillets were dried by hot air drying (HD, at 35 and 45°C) and vacuum microwave drying (VMD, at 1, 4, and 7 W/g) to a final moisture content. Compared with hot air drying, the vacuum microwave drying greatly reduced drying time. The rehydration rate constant of salted grass carp fillets dried by microwave vacuum drying at 7-W/g microwave intensity was significantly higher than that of the hot air drying at 35 and 45°C. Compared with hot air drying, the vacuum microwave dried samples had a higher crude fat content (dry basis). The lightness of the samples dried by vacuum microwave drying was higher, and the yellowness was lower than those dried by hot air drying. The drying methods had no significant effect on the hardness and springiness.     

Effects of Freshness and Heating Temperature on the Discoloration of Squid Meat Products

ABSTRACT

Squid processed products such as dried-seasoned squid turn brown during storage, causing a change in quality. The main cause of this browning is melanoidin produced by the Maillard reaction. In this study, four types of squid were processed at different heating temperatures between 50 and 90°C and examined for suppression of the production of D-ribose, the main component of melanoidin. Furthermore, the color change of the squid meat with each heat treatment during storage of 32 days at 30°C was investigated. Bigfin reef squid and cuttlefish with a relatively high freshness decreased D-ribose generation as the heating temperature increased, and the browning during storage also decreased accordingly. In contrast, Humboldt squid with a low freshness did not generate new D-ribose at any heating temperature during storage. Nevertheless, the browning was reduced as the heating temperature increased. It is thought that the Maillard reaction was also reduced by the heat denaturation of muscular proteins. These results suggest that browning due to the Maillard reaction of squid meat is greatly affected by initial freshness and heating temperature.     

Feasibility study on water solubilization of spawned out salmon meat by conjugation with alginate oligosaccharide

A feasibility study was carried out to determine whether water-soluble salmon meat could be manufactured by conjugating a glycosyl unit using the Maillard reaction. Spawned out salmon meat was washed, mixed with alginate oligosaccharide (AO) and sorbitol, lyophilized, and then heated at 60°C and 5–95% relative humidity (RH) to introduce AO (the mean degree of polymerization was six) into the myofibrillar proteins through the Maillard reaction. The reaction progressed with an increase in the reaction humidity and the amount of AO bound to the protein reached >150 μg/mg at RH 65 and 90%. However, the protein glycosylation under high humidity impaired protein solubility and the meat protein became effectively water-soluble with the conjugation with AO at reaction conditions of 60°C and RH 35%. The improved characteristics of the meat protein were highly stable at room temperature. Further, the water-soluble protein can be prepared from the frozen salmon meat stored at −25°C for 60–90 days. These results indicate that protein glycosylation has strong potential for use with spawned out chum salmon. The suppression of protein denaturation during processing is important to obtain the high water-soluble meat protein.     

Quality Changes and Browning Developments During Storage of Dried-Seasoned Squid (Dosidicus gigas and Ommastrephes bartrami)

ABSTRACT

The physicochemical changes—moisture, water activity, crude protein, total volatile basic nitrogen (TVB-N), trimethylamine oxide (TMAO), dimethylamine (DMA) and formaldehyde, reducing sugar and amino acid; microbiological changes—aerobic plate count (APC) and coliforms; and browning development (browning intensity and color) in dried-seasoned squids (Dosidicus gigas and Ommastrephes bartrami) during storage at 25°C were investigated. After the storage, the moisture and water activity decreased significantly. The content of crude protein decreased, accompanied by progressive accumulation of TVB-N and slow increase of APC. The high level of TMAO in dried-seasoned squid gradually decreased, whereas DMA and formaldehyde increased significantly during the whole period of storage. The browning intensity and b* value significantly increased, which revealed the occurrence of browning in two squid products. The dominant reducing sugar, lactose, and glucose, and the total amino acid including lysine and proline, decreased remarkably, which were well-correlated with the browning development. The quality deterioration in jumbo squid was more rapid than in neon flying squid partially due to the high level of reducing sugar in the former product. To improve quality and resist browning reaction of dried squid product during storage, the addition of extrinsic reducing sugar became a control factor.     

Drying Kinetics and Quality Characteristics of Indian Mackerel (Rastrelliger kanagurta) in Solar–Electrical Hybrid Dryer

The aim of the study was to investigate the drying kinetics and quality characteristics of Indian mackerel dried under solar–electrical hybrid dryer (S-EHD). Fresh Indian mackerel fishes (Rastrelliger kanagurta) were cleaned, cut into butterfly fillets, and salted overnight using a dry salting method (salt-to-fish ratio, 1:3). The salted mackerel was dried in a S-EHD at the air temperature of 45–55°C, relative humidity of 47–62%, and air velocity of 0.60–0.80 m/s. Open sun drying (OSD) of salted Indian mackerel was conducted to compare with S-EHD. The moisture content of the salted mackerel (61.5% w.b.) was reduced to 31.8% (w.b.) under S-EHD and 30.25% (w.b) under OSD in 8 and 32 h, respectively. The drying rate curve showed that mackerel drying occurred under falling-rate drying period in both the drying methods. A drying efficiency of 23.81% was observed for salted Indian mackerel drying under S-EHD. Diffusion approach and two-term models were selected to accurately predict the drying behavior of mackerel under S-EHD and OSD, respectively. Total volatile base nitrogen (TVB-N), trimethylamine, and thiobarbituric acid analysis of dried samples revealed that the mackerel dried under S-EHD was better than OSD. In the sensory analysis, samples dried under S-EHD recorded highest overall acceptability score.     

Change in translucency of squid mantle muscle upon storage

ABSTRACT:   Change in the translucency of squid mantle muscle during itsstorage at 0°C was studied by monitoring L* valueand turbidity. Two indicators showed that squid mantle muscle lostits translucency and reached maximal turbidity within 24 hof storage. Thickness of mantle muscle also increased by 15% in12 h, earlier than the loss of translucency. ATP contentdecreased with storage time and was completely lost in almost 24 h,a similar period to translucency loss, but later than rigor contraction.Development of black color on skin surface by chromatophores wasfully achieved in 24 h. It was thus concluded that ATPcontent was well correlated with a loss of translucency or increasein the turbidity of mantle muscle and development of dark coloron the surface skin of mantle.     

栅栏技术优化即食调味珍珠贝肉工艺的研究

文章运用栅栏效应的理论，确定了高水分型即食调味珍珠贝肉食品的制作工艺，分析了各种常见栅栏因子对制品感官品质及微生物的影响，优化前处理、烘干工艺、杀菌工艺、pH、水分活度（activity water，Aw）和低温处理等多种栅栏因子，形成有效防止制品腐败变质的栅栏模式。试验结果表明，原料前处理需用3％的食盐进行了清洗；采用0．15％柠檬酸溶液调节pH为5．6～5．7；贝肉调味后需经烫煮；产品水分含量45％～50％，Aw为0．88～0．90；产品真空包装，低温处理24h，再采用巴氏杀菌（80～85℃、30min），能较好地保持产品的品质和风味，延长保存期，保持珍珠贝肉特有的鲜味和营养价值，促进珍珠贝肉的开发利用。     

Effect of drying on the viability of the probiotic bacterium Lactobacillus plantarum Lab9 (CPQBA 144‐09 DRM 03) impregnated in the feed for tilapia (Oreochromis sp.)

Drying experiments were conducted at different temperatures and air flux velocities to determine the proper drying conditions for reducing moisture in commercial fish feed impregnated with probiotic lactic acid bacteria and to assess the effect on bacteria viability over time. At temperatures of 45°C, the drying time was shorter, without the air flux velocity under study having a relevant influence. The drying conditions influenced the viability of the bacteria in the feed; the least loss of viability was obtained with a velocity of 0.8 m s^?1 and a temperature of 45°C during 15 min. Using these drying conditions, 5 kg of feed was dried and stored for a month at temperature of 26°C and relative humidity of 75%. The viability of the bacteria and the moisture of the feed were measured every 3 days during the storage period. Loss of viability followed first order kinetics, with a constant k of 0.112 days^?1. Thus, the viability of the bacteria in the feed is less than 10⁶ CFU g^?1 after 43 days.     

Characteristics of burnt meat in cultured yellowtail <Emphasis Type="Italic">Seriola quinqueradiata</Emphasis>

ABSTRACT:   In order to understand the characteristics of burnt meat in cultured yellowtail Seriola quinqueradiata , fish were kept at two different temperatures (13 and 30°C) and slaughtered by either spinal cord destruction (SCD) or suffocation in air (SA). Early postmortem changes during storage at 32°C were analyzed by rheological, biochemical, and histological methods. The burnt meat (with lightness parameter, L* ≥ 55) was observed at 1-h storage in the SA 30°C group, at 2 h in SCD 30°C, and at 4 h in SA 13°C; meat was normal for the SCD 13°C group until 6 h of storage. Breaking strength scores were higher for the normal meat (200 g/cm²) than burnt meat (70 g/cm²) at 4 h of storage. Expressible water content was higher for the burnt meat than for the normal meat. Adenosine triphosphate concentrations for the SCD groups were higher than for the SA counterparts. Moreover, pH decrease was much faster in the 30°C groups, showing pH 5.6 at 2 h of storage. A negative correlation between the pH and lactic acid contents in muscle ( P  < 0.001) was found. Histological analysis evidenced a larger pericellular area (40%) in the burnt samples than in the normal samples (16%). It was confirmed that a higher fish-keeping water temperature and a stressful slaughter method (faster glycolytic process) were determinative factors that influence the occurrence of burnt muscle in yellowtail, and that the effect of the former is larger than the latter.     

Effects of Drying Condition on Physico-chemical Properties of Foam-mat Dried Shrimp Powder

ABSTRACT

The effects of drying parameters on moisture content, water activity, bulk density, water binding capacity (WBC), oil absorption, and color parameters (L*,a*, b*) of foam-mat dried shrimp powders were investigated. Shrimp purees were dried as a foam mat at different drying temperatures (50ºC, 60ºC, and 70ºC) and thicknesses of the foam layer (4 and 8 mm). The experimental results showed that as the temperature increased, the moisture content, water activity, and oil absorption of the samples decreased. However, their solubility increased significantly (P ?0.05). Bulk density of shrimp powder increased with increasing the thickness of the foam layer. Increasing temperature led to increased and decreased WBC with 4 and 8 mm thickness, respectively. Furthermore, the a*, b*, and L* (at 8 mm thickness) decreased with increasing temperature; however, L* increased at 4 mm thickness. Fourier transform infrared (FTIR) spectra showed some major shifts, such as in the region of 1000–1700 cm^?1, related to C–O, C–O–C, and C–H band stretching. The differential scanning calorimetry thermograms showed a large peak with a denaturation peak around 72ºC for shrimp proteins.     

Physiochemical and Textural Alterations in Indian Squid (Loligo duvauceli) Mantle During Frozen Storage and Cooking

Textural variations in squid mantle and the role of proteins on texture during frozen storage and cooking were investigated. Myofibrillar protein (62.36%) and pepsin soluble collagen (10.70%) accounted for the major fraction of total protein (22.17%). The histochemistry of mantle tissue showed a mesh-like arrangement of myofibrillar proteins with a collagenous dermal layer and feebly passing collagen through myotome bundles. Texture profile analysis of unfrozen mantle suggested the first phase of hardening at 50°C with hardness 1 (H1) of 11.53 kgf and hardness 2 (H2) of 9.68 kgf; and the second phase of hardening with optimal texture and maximum juiciness at 70°C (H1, 8.11 kgf; H2, 7.13 kgf) that varied with extended frozen storage. Increased frozen storage and cooking led to protein denaturation and formation of new low molecular weight proteins as evidenced in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE); these possibly influenced the functional and microstructural properties of the tissue.     

Denaturation mode of carp myofibrils when affected by temperature and salt concentration

ABSTRACT: Heating temperatures of 30–40°C and KCl concentrations of 0.1–0.5 M altered the denaturation mode of carp myofibrils. In 0.1 M KCl medium, heating temperature affected the denaturation of rod more significantly than of subfragment-1 (S-1), and a slow decrease in solubility at 30°C was accompanied by a slow denaturation of rod. KCl concentration at heating altered the denaturation mode differently at 30°C and 40°C. Increased KCl concentrations for heating reduced the rod denaturation rate at 40°C, but it was increased at 30°C. At concentrations above 0.3*Τ*M KCl, the denaturation rate for rod became identical to that for S-1 at both temperatures. Upon heating of chymotryptic digest of myofibrils, S-1 denaturation was similarly detected as in intact myofibrils, whereas practically no rod denaturation was detected. Thus, it was concluded that myosin structure connecting S-1 and rod has an important role in the denaturation process.     

Development of Intermediate Moisture Fish Patties from Minced Rockfish Meat (Sebastes sp.)

Abstract

An Intermediate Moisture (IM) fish patty was formulated from rockfish mince (85.7%), sorbitol (6.0%), soy protein isolate (2.0%), tapioca starch (2.0%), chicken bouillon (1.3%), refined salt (2.5%), and dried seasonings (0.5%). The mixture was dehydrated at 40 °C for 10 h and vacuum packaged. The dried product had a moisture content of 33% and a water activity of 0.82. The desorption isotherm was sigmoid in shape indicating water activity of the product at ambient temperature in the Philippines would remain stable. Seasonings were added to improve the taste and possibly the stability of the product. Sorbitol addition improved the texture, as judged by a sensory panel, as well as the rehydration index of the patties. Humectat and seasonings improved the overall acceptability of the patties by a sensory panel. Product was prepared with and without preservatives (potassium sor-bate and TBHQ), dehydrated at 40 °C for 10 h, vacuum packed and held at 38°C or ?20°C for two months. Moisture content, water activity, pH, rehydration index, TEA value, Hunter-Gardner color, texture profile, amino acid composition, sensory properties and microbial growth were determined at storage intervals. Instrumental and sensory tests showed no significant differences (p < 0.05) among samples stored with or without preservative and at ?20°C vs. 38°C.     

Assessing the end-point temperature of heated fish and shellfish meats

ABSTRACT: Attempts have been made to assess the end-point temperature (EPT) of heated fish and shellfish meats by using the coagulation method together with sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis and enzyme activity determination. Unfrozen and frozen fish and shellfish meats were heat-treated at different selected temperatures with 0, 15 or 30 min holding times. Proteins were extracted with NaCl solution. The coagulation method was able to determine EPT of heated fish and shellfish meats between 60 and 67°C. SDS-PAGE patterns of the filtrates from heated meats were closely related to the results of the coagulation method and enzyme activity determination. Two proteins responsible for producing coagulum of fish meat extracts seem to be lactate dehydrogenase (LDH) and glyceraldehyde phosphate dehydrogenase (GAPDH). End-point temperatures determined by these methods were not significantly different between unfrozen and frozen samples. On the contrary, a highly thermostable protein with a molecular mass of 35 kDa was detected in heated shellfish meats up to 108°C. In scallop adductor muscle, this highly thermostable protein was found to be the tropomyosin subunit from its amino acid composition and their partial sequences. Tropomyosin could be used as an EPT indicator up to 108°C for heated shellfish meats.     

鲍鱼冷风干燥和自然晾晒试验的比较分析

研究皱纹盘鲍(Haliotis discus hannai Ino)在冷风干燥和自然晾晒两种干燥方式下的干燥速率、色泽、酸性粘多糖和总糖含量,以及微观结构变化的差异,并进行分析比较。在实验室内采用冷风干燥试验机和自然晾晒方式进行鲍鱼干燥试验。结果显示:当鲍鱼样品干基含水率从238%下降到16%以下时,冷风干燥所需时间约为122 h,是自然晾晒的70%;至干燥完成时冷风干燥鲍鱼多糖含量高于自然晾晒样品;冷风干燥样品通透性明显优于晾晒样品;两种干燥样品的微观结构差异性不明显。综合分析得出,与自然晾晒相比,冷风干燥方式具有干燥时间短,干燥过程易于控制,品质和卫生条件好,适合工业化生产等优点,是一种较佳的鲍鱼干制方法。     

Comparative Properties of Bluefish (Pomatomus saltatrix) Gels Formulated by High Hydrostatic Pressure and Heat

High pressure was applied at levels of 300 to 3,742 atm for 30 min to formulate gels from bluefish meat paste, and the properties of the resulting gels were compared with those of heat-induced gels formulated at 90°C for 20 min, or at 60°C for 60 min. The moisture content of the pressure-induced gels was similar to that of the heat-induced gels, while protein contents and pH values of pressurized gels were slightly lower than those formulated by heat. Gels formed by pressure were more translucent as compared with those formulated by heat. Texture measurement indicated that there were no significant differences between the elasticities of the gels obtained under various pressures, although gel firmness increased with pressure. Overall, the heat-induced gels formulated at 90°C for 20 min were firmer but had similar elasticities to pressure-induced gels, while gels formulated at 60°C for 60 min were comparatively softer and had lower elasticities. The salt-extractable protein and protein digestibility studies indicated that pressure treatment formed gels with less protein denaturation and which were more digestible than the fish gels formulated by heat. The results from proteolytic activity studies showed that the pressure range used in this study was less effective in inactivating the endogenous proteases in the fish flesh than heat.     

Chemical, physical and sensory changes of small abalone meat during cooking

ABSTRACT:   Small abalone meats were heated at 80°C and 98°C for 0–120 min and the differences in chemical, physical and sensory changes of the cooked meats were investigated. The decrease in moisture and weight and the increase in browning and Hunter's b -value were relatively higher for cooking at 98°C than at 80°C. After cooking for 20–120 min, the total amount of adenosine triphosphate and its related compounds on a dry weight basis decreased by 17–27% at 80°C and by 30–39% at 98°C; the total amount of free amino acids on a dry weight basis changed insignificantly at 80°C but decreased by 22–35% after cooking at 98°C. The meats cooked at 80°C were higher in cutting force whereas the levels in the samples cooked at 98°C did not decrease until samples had been cooked for 60 min. The hydroxyproline content showed little change during cooking except for in samples cooked at 98°C for 120 min, in which the content was found to be low. The extended cooking at 80°C improved the acceptability of small abalone meat, whereas only the acceptability score of aroma increased significantly for cooking at 98°C.