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Erythrocyte pyruvate kinase (PK) deficiency is an inherited glycolytic erythroenzymopathy caused by mutations of the PKLR gene. A causative mutation of the feline PKLR gene was originally identified in Abyssinian and Somali cats in the U.S.A. In the present study, a TaqMan probe-based real-time PCR genotyping assay was developed and evaluated for rapid genotyping and large-scale screening for this mutation. Furthermore, a genotyping survey was carried out in a population of four popular purebred cats in Japan to determine the current mutant allele frequency. The assay clearly displayed all genotypes of feline PK deficiency, indicating its suitability for large-scale survey as well as diagnosis. The survey demonstrated that the mutant allele frequency in Abyssinian and Somali cats was high enough to warrant measures to control and prevent the disease. The mutant allele frequency was relatively low in Bengal and American Shorthair cats; however, the testing should still be carried out to prevent the spread of the disease. In addition, PK deficiency should always be considered in the differential diagnosis of anemia in purebred cats in Japan as well as worldwide.  相似文献   
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牙鲆在保藏过程中影响ATP关联化合物降解的因素   总被引:3,自引:2,他引:1  
为了深入了解牙鲆体内ATP关联化合物的变化,尤其是肌苷酸(IMP)降解这一限速步骤,利用高效液相色谱法(high efficiency liquid chromatography,HPLC)测定不同保藏条件下牙鲆体内ATP关联化合物和K值变化,并探讨了影响其变化的因素。结果显示,0°C保藏条件下牙鲆体内ATP在1 d内迅速降解,产生IMP作为初期的主要积累物质,蓄积一段时间(10 d)后迅速降解为次黄嘌呤(Hx),IMP的降解过程呈现二相性;鱼肉制成鱼糜形态保藏,ATP 0 d时几乎降解完全,但IMP的降解过程仍旧呈现二相性;加入抗菌素的鱼肉中微生物的生长得到有效抑制,此时IMP降解缓慢并且呈线性下降,原本的蓄积过程消失,K值的增长速率也随之减慢;加入Tritonx-100破坏细胞膜结构发现,牙鲆体内IMP的降解速率加快,同时K值的增长速率也随之加快。结果表明,牙鲆的保藏形态影响ATP的降解速率,但几乎不影响其他关联产物的降解变化;保藏后期外源微生物产生的酶与内源酶的叠加作用是导致保藏后期IMP加速降解、从而呈现二相性的原因,微生物是影响ATP关联化合物降解的关键因素;表面活性剂通过改变细胞膜结构也能够影响IMP的降解过程。  相似文献   
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ABSTRACT:   Change in the translucency of squid mantle muscle during itsstorage at 0°C was studied by monitoring L* valueand turbidity. Two indicators showed that squid mantle muscle lostits translucency and reached maximal turbidity within 24 hof storage. Thickness of mantle muscle also increased by 15% in12 h, earlier than the loss of translucency. ATP contentdecreased with storage time and was completely lost in almost 24 h,a similar period to translucency loss, but later than rigor contraction.Development of black color on skin surface by chromatophores wasfully achieved in 24 h. It was thus concluded that ATPcontent was well correlated with a loss of translucency or increasein the turbidity of mantle muscle and development of dark coloron the surface skin of mantle.  相似文献   
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KUNIHIKO  KONNO  CHO  YOUNG-JE  TAKEYA  YOSHIOKA  PARK  SHINHO  NOBUO  SEKI 《Fisheries Science》2003,69(1):204-209
ABSTRACT:    Jumbo squid was very similar to Japanese common squid in terms of myofibrillar Ca2+-, Mg2+- and K+(EDTA)-ATPase activities. Myofibrils of jumbo squid were significantly stabilized upon addition of Ca2+ and destabilized by increasing KCl concentration for heating. Incubation of muscle homogenate of jumbo squid induced a selective cleavage of myosin into two major fragments and the cleavage was inhibited by EDTA. Autolysis was prominent at and above 0.3 M NaCl where myosin filaments dissolve. The enzyme involved in the autolysis was proved to be unstable showing maximal autolysis rate at 25°C. Washing the homogenate partially reduced the autolysis activity.  相似文献   
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鲤鱼鱼鳞胶原蛋白ASC与PSC的比较   总被引:1,自引:0,他引:1  
以鲤鱼鱼鳞为原料,研究了酸法和酶法提取的鱼鳞胶原蛋白ASC和PSC的异同.经过SDS-凝胶电泳和氨基酸分析可以看出,2种蛋白在分子构型、氨基酸组成等方面的差异并不明显,经过蛋白酶K有限酶解后的图谱证明了这一点.但从差热分析,以及蛋白质受热的分解变化来看,二者之间在热稳定性方面存在一定的差异.推测差距产生的原因与蛋白质制备过程中,胃蛋白酶的作用有关,胶原蛋白两端的非螺旋区域受到酶的作用而分解,非螺旋区域对三螺旋的稳定性起着重要作用,非螺旋区域的分解大大降低了PSC对热的耐受性.  相似文献   
9.
ABSTRACT: Heating temperatures of 30–40°C and KCl concentrations of 0.1–0.5 M altered the denaturation mode of carp myofibrils. In 0.1 M KCl medium, heating temperature affected the denaturation of rod more significantly than of subfragment-1 (S-1), and a slow decrease in solubility at 30°C was accompanied by a slow denaturation of rod. KCl concentration at heating altered the denaturation mode differently at 30°C and 40°C. Increased KCl concentrations for heating reduced the rod denaturation rate at 40°C, but it was increased at 30°C. At concentrations above 0.3*Τ*M KCl, the denaturation rate for rod became identical to that for S-1 at both temperatures. Upon heating of chymotryptic digest of myofibrils, S-1 denaturation was similarly detected as in intact myofibrils, whereas practically no rod denaturation was detected. Thus, it was concluded that myosin structure connecting S-1 and rod has an important role in the denaturation process.  相似文献   
10.
The prolactin gene family represents species‐specific expansions of hormones/cytokines contributing to the regulation of pregnancy‐dependent adaptations. In this review, we discuss the prolactin family expansion in the mouse and rat, its linkage with viviparity and its potential role in regulating adaptations to physiological stressors.  相似文献   
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