贵州省开阳县某猪场主要猪病的血清学抗体检测分析

为了解贵州省开阳县某猪场猪繁殖与呼吸综合征病毒、猪瘟病毒、猪O型口蹄疫病毒、猪伪狂犬病病毒的免疫及感染情况,通过采用ELISA检测方法对猪场30份血清进行相关抗体检测。结果:(1)猪O型口蹄疫病毒、猪伪狂犬病毒gB、猪瘟病毒免疫抗体合格率均>70%,免疫效果较好。(2)猪口蹄疫3-ABC抗体阳性率为0,表明猪群无口蹄疫病毒感染。(3)猪繁殖与呼吸综合征病毒免疫抗体合格率为26.7%,未达到国家农业农村部规定≥70%的要求,需加强免疫。(4)猪伪狂犬病病毒gE抗体阳性率为70%,表明猪群存在伪狂犬病病毒感染。结论:猪场需加强猪繁殖与呼吸综合征的免疫和防控,同时要做好猪伪狂犬病的检疫与净化工作。     

清远市部分规模化猪场四种疫病的抗体检测与分析

为评价清远市部分规模化猪场猪瘟(CSF)、猪繁殖与呼吸综合征(PRRS)、猪圆环病毒病(PCVD)和猪伪狂犬病(PR)疫苗免疫水平,从7家规模化猪场采集了322份血清样品,采用酶联免疫吸附试验(ELISA)方法检测了猪瘟、猪繁殖与呼吸综合征、猪圆环病毒病和猪伪狂犬病病毒gB抗体和gE抗体。结果表明:猪瘟、猪繁殖与呼吸综合征、猪圆环病毒病和猪伪狂犬病病毒gB抗体阳性率分别为93.48%、89.13%、90.37%、92.24%,离散度分别为22.69%、56.61%、36.13%、31.12%。说明这7家猪场四种疫病疫苗免疫效果良好,其中有1家猪场怀疑伪狂犬野毒流行。     

规模化猪场主要疫病监测及防制

本试验采用酶联免疫吸附试验（ELISA）、正向间接血凝试验（IHA）和乳胶凝集试验（LAT）对曲靖市 16个规模化猪场的2751份血清的8种猪病血清抗体进行了检测。检测结果显示,猪瘟病毒（HCV）、猪O型口蹄疫非结构蛋白（NS）、猪伪狂犬病病毒 gE基因（ADV gE）、猪细小病毒（PPV）、猪日本乙型脑炎病毒（JEV）、猪繁殖与呼吸综合征病毒（PRRSV）、猪圆环病毒2型（PCV 2）和布鲁氏杆菌的血清抗体阳性率分别为0%、0%、22.28%、32.93%、52.99%、29.96%、21.88%和0%;猪瘟（HC）、猪O型口蹄疫（FMD）、猪繁殖与呼吸综合征（PRRS）免疫血清抗体阳性率分别为59.43%、64.56%、48.55%。结果表明,所检测的规模化猪场未感染猪瘟、O型口蹄疫、布鲁氏杆菌病野毒,但存在不同程度地猪伪狂犬病病毒、猪细小病毒、猪日本乙型脑炎病毒、猪圆环病毒2型、猪繁殖与呼吸综合征病毒感染;猪瘟、O型口蹄疫、猪繁殖与呼吸综合征总体免疫效果不佳。     

2011～2014年上海市屠宰场上市肉猪主要病毒感染情况调查

为了解上海市屠宰场上市肉猪主要病毒的感染情况,2011~2014年在上海市5个屠宰场共采集759份血清样品,采用ELISA方法进行了口蹄疫、猪瘟、猪繁殖与呼吸综合征抗体检测;采用荧光PCR方法对720份组织样品进行了口蹄疫病毒、猪繁殖与呼吸综合征病毒、猪瘟病毒、伪狂犬病毒、猪圆环病毒2型、猪流感病毒、猪细小病毒7种病毒检测。血清学检测结果显示,猪繁殖与呼吸综合征、猪瘟、口蹄疫抗体平均阳性率依次为96.6%、72.3%、66.4%。荧光PCR检测结果:口蹄疫病毒、猪瘟病毒、猪繁殖与呼吸综合征病毒、伪狂犬病毒均为阴性,但猪圆环病毒2型、猪细小病毒阳性率较高,阳性率分别是37.9%、8.3%。由此可见,上海市屠宰场上市肉猪重大动物疫病防控已有一定效果,但猪圆环病毒2型和猪细小病毒仍具有较高阳性率,对猪群健康存在一定的危害。     

5省区规模猪场猪呼吸道病综合征病原学调查的统计分析

对5省区规模猪场猪呼吸道病综合征(PRDC)的2 184份血清检测结果进行分析、归纳,结果显示,其主要病原是猪繁殖与呼吸综合征病毒、圆环病毒2型和伪狂犬病病毒,其中猪繁殖与呼吸综合征病毒阳性率为34.80%,圆环病毒2型阳性率为58.24%,伪狂犬病病毒阳性率为27.79%,而猪瘟病毒和猪流感病毒的感染率较低;上海市病料的主要病因是肺炎支原体(57.50%)和猪繁殖与呼吸综合征病毒(45.83%),海南省血清的主导病原是圆环病毒2型(90.5%)、胸膜肺炎放线杆菌(72.7%),说明肺炎支原体和胸膜肺炎放线杆菌的感染可能有一定的地域性;PRDC的混合感染率较高(广西43.77%,云南35.75%)。     

云南地区部分猪场猪呼吸道疾病综合征致病病毒感染的流行病学调查

为了解云南地区猪场猪呼吸道疾病的流行病学,从而为有效防制该疾病提供科学依据,利用ELISA和RT-PCR方法检测了春、夏、秋、冬四季,云南地区不同区域规模养殖场1 164份血清和全血样品中的猪瘟抗原、猪繁殖与呼吸综合征抗原、猪伪狂犬gE抗体、猪圆环病毒2型特异抗体。结果显示:猪瘟抗原阳性率为11.34%,猪圆环病毒2型抗体阳性率为50.86%,猪伪狂犬病病毒gE抗体阳性率为35.40%,猪繁殖与呼吸综合征抗原阳性率为44.51%。表明云南地区规模化猪场存在猪瘟病毒、猪圆环病毒2型、猪伪狂犬病野毒、猪繁殖与呼吸综合征病毒感染,不同季节和猪群年龄阶段感染情况有一定差异性。     

重庆某种猪场猪瘟、口蹄疫、伪狂犬病、猪繁殖与呼吸综合征抗体的ELISA检测

利用ELISA方法,对重庆市渝北区某种猪场种公猪、保育猪、育成猪、后备种猪、基础母猪不同类型和生长阶段猪群的猪瘟病毒抗体、O型口蹄疫抗体、口蹄疫病毒非结构蛋白抗体、伪狂犬病抗体、猪伪狂犬病病毒gpI抗体、猪繁殖与呼吸综合征病毒抗体进行了监测和分析,结果表明:猪瘟、口蹄疫、猪伪狂犬病免疫总体水平较高,在上半年和下半年的抗体检测合格率均达到100%;猪繁殖与呼吸综合征病毒在上半年和下半年的抗体平均阳性率分别为9.8%和62%,抗体阳性率分别在95%～100%和40%～80%,由于该种猪场未曾免疫猪繁殖与呼吸综会征疫苗,表明猪繁殖与呼吸综合征病毒的感染程度较高,且在不同猪群普遍存在,应予以充分重视。     

一起仔猪四重病毒感染的诊治

为了解贵州省都匀市某规模化猪场发病情况,利用RT-PCR和PCR法对送检的病料进行猪流行性腹泻病毒、猪传染性胃肠炎病毒、猪瘟病毒、猪繁殖与呼吸综合征病毒、猪圆环病毒2型和猪伪狂犬病病毒进行快速检测。根据临床症状、病理变化及实验室检测结果,综合诊断该猪场存在猪流行性腹泻病毒、猪瘟病毒、猪繁殖与呼吸综合征病毒和猪圆环病毒2型的混合感染。     

驻马店市猪群主要疫病流行情况调查

<正>为了解当前驻马店市猪群主要疫病流行情况,掌握猪群主要发病及死亡原因,定期从市内3个无害化处理厂采集病死动物组织样品,进行猪瘟、猪繁殖与呼吸综合征(猪蓝耳病)、口蹄疫、猪伪狂犬病、猪圆环病毒病2型、猪圆环病毒病3型、猪细小病毒病、猪传染性胃肠炎、猪流行性腹泻、副猪嗜血杆菌病、     

常见猪病流行概况与防控

最近几年河北省常见猪病情况主要有以下几种:猪瘟、伪狂犬、猪口蹄疫、病毒性腹泻、副猪嗜血杆菌、圆环病毒、猪繁殖与呼吸综合征(蓝耳病)。下面笔者就这几种猪病的流行特点与防控浅谈以下自己的体会:1伪狂犬病1.1流行情况2005年~2011年初,伪狂犬流行趋于下降趋势;     

Heartworm in dogs in Canada in 1983

In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario.

Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously.

     

Scrapie in goats in Cyprus

Ovine scrapie was first recorded in Cyprus in 1985. Subsequently four dairy goats kept in two mixed flocks with affected sheep developed characteristic clinical signs similar to those seen in sheep. Fifteen goats from the two flocks were examined histologically and neurological lesions consistent with a diagnosis of scrapie were found in the four animals and in three others which had subsequently developed early neurological signs. These lesions were similar to those of naturally-affected sheep although neuronal degeneration and vacuolation were more severe in some cases.     

Heartworm in dogs in Canada in 1985

In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).

Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.

     

Heartworm in dogs in Canada in 1988

In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%).     

Heartworm in dogs in Canada in 1984

In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.

Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.

     

Heartworm in dogs in Canada in 1991

In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium.     

Heartworm in dogs in Canada in 1989

In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.

Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.