猪链球菌病的流行与诊治

<正>猪链球菌病是人畜共患的急性细菌性传染病,由C、D、E及L群链球菌引起的猪的多种疾病的总称。猪链球菌病主要表现为猪败血性和局灶性淋巴结化脓性病症。该菌不仅可以引发猪的急性败血症、脑膜炎、关节炎及急性死亡等,还可通过伤口、消化道等途径传给特定人群,甚至导致人死亡,危害公共安全卫生。1流行病学猪链球菌病是二类动物传染病,病猪和隐性感染猪是该病的主要传染源。其流行无明显季节性,但     

猪链球菌病灭活疫苗安全性与免疫效力试验

我国学者对猪链球菌致病菌的研究证实,国内猪链球菌病的病原主要是C群和D群链球菌,而国外多由R群Ⅱ型链球菌引起猪发生败血型和脑炎型链球菌病.近年来,许多猪场的猪群经猪链球菌疫苗免疫后,猪链球菌病仍时有发生,对分离的致病菌做了分群鉴定后发现,用于免疫疫苗的疫苗株的菌群与当地的流行株不符,结果造成免疫失败.因此在做好该病病原流行株调查基础上,筛选有代表性的、免疫原性好的链球菌作为制苗的疫苗株,研制适合本地区的多价灭活疫苗,已经成为控制该病的有效手段.     

猪链球菌病的流行与诊治

猪链球菌病是人畜共患的急性细菌性传染病,由C、D、E及L群链球菌引起的猪的多种疾病的总称。猪链球菌病主要表现为猪败血性和局灶性淋巴结化脓性病症。该菌不仅可以引发猪的急性败血症、脑膜炎、关节炎及急性死亡等,还可通过伤口、消化道等途径传给特定人群,甚至导致人死亡,危害公共安全卫生。1流行病学猪链球菌病是二类动物传染病,病猪和隐性感染猪是该病的主要传染源。     

一起猪链球菌病的防治

猪链球菌病是一种人畜共患的急性、热性传染病。该病流行无明显的季节性,一年四季均可发生。临床上主要表现为淋巴结脓肿、脑膜炎、关节炎以及败血症为主要特征。同时可通过破损皮肤如伤口或擦伤传染给人,也可通过呼吸道感染人,严重感染时可引起人的死亡。笔者多年在基层从事畜牧兽医工作,现将2008年5月发生的一起病例诊治情况介绍如下,...     

猪链球菌病的诊断与防治

猪链球菌病(Swine Streptococcosis)是世界各地猪的一种常见传染性疾病,危害严重。该病在我国广大的牲猪养殖区域广泛流行,造成巨大的经济损失,同时危害从业人员的健康。1流行病学1945年,Bryante报道在母猪和仔猪当中发生的一种由链球菌引起的败血性传染性流行病。目前,美、英、法、日、俄、印度、丹麦、荷兰、加拿大等国家均有猪链球菌病的报道。1998～1999年连续两年的夏季在江苏省南通地区,猪群大面积暴发流行2型猪链球菌病,猪群的发病率和死亡率均在50%左右。特别是2005年的四川猪链球菌感染人事件最为严重。本病多年来一直困扰我国养…     

中西医治疗猪链球菌病

猪链球菌病是生猪养殖环节中常发的疫病之一,属于我国三类动物疫病,该病对仔猪威胁较大,严重影响了生猪养殖产业的规模化发展,尤其是基层养殖户的管理模式比较粗放,经常会造成该病的大规模流行,极易形成疫区。同时猪链球菌也是一种可感染人的病原菌,其中猪链球菌血清2型感染人的事件常见报导。因此,必须加强对该病的认识并采用有效治疗方法。主要分析中西医治疗猪链球菌病,并提出了相应的预防措施,为生猪健康养殖提供一定的参考依据。     

猪链球菌病的诊治

猪链球菌病分为猪败血性链球菌病、猪链球菌性脑膜炎、淋巴结脓肿和关节炎等类型,以哺乳和断奶仔猪最易感.据资料报道,猪体内猪链球菌的带菌率一般为20％～40％,正常情况下呈隐性感染,如细菌产生毒力变异,则该病往往可引起生猪的大批死亡,给养猪业带来巨大经济损失.     

猪链球菌病的诊断与防治

猪链球菌病是一种常见的猪传染病,该病的流行或扩散给我国养猪业造成了巨大的经济损失,并可能引起从业人员感染。分析了如何通过临床症状和实验室技术对该病进行诊断,并提出了治疗和预防的措施。     

猪弓形体病的症状及防治方法

猪弓形体病是由弓形体感染动物或人引起的一种原虫病,又称为弓形虫病,该病在养猪场中非常常见,一旦发病,就会给养猪业造成巨大的经济损失。从侵入途径来看,弓形虫主要通过口、眼、鼻腔、呼吸道、肠道等途径侵入到猪的体内,患病猪通常会表现出高热、呼吸困难等症状。由于该病和猪瘟、猪链球菌病的症状非常相似,所以在临床诊治时很容易被误诊为猪链球菌病、猪瘟等。     

齐齐哈尔地区猪链球菌病病原菌的分离鉴定及药敏试验

猪链球菌病主要是链球菌引起的猪重要疾病,猪不分品种、年龄阶段均容易感染,并且能够传染给人,可致人和猪死亡。猪链球菌主要感染1～12周龄仔猪,呈地方性流行,并在世界范围内造成巨大损失。为了给齐齐哈尔地区的猪链球菌病提供第一手资料,我们通过采集病料进行了细菌分离,得到菌株,通过培养特性、形态染色、生化试验、动物致病性试验,鉴定出猪链球菌6株,并进行了药物敏感试验,结果报告如下。1材料与方法     

Streptococcus suis infections in humans: the Chinese experience and the situation in North America

Infections caused by Streptococcus suis are considered a global problem in the swine industry. In this animal species, S. suis is associated with septicemia, meningitis, endocarditis, arthritis and, occasionally, other infections. Moreover, it is an agent of zoonosis that afflicts people in close contact with infected pigs or pork-derived products. Although sporadic cases of S. suis infection in humans have been reported, a large outbreak due to S. suis serotype 2 emerged in the summer of 2005 in Sichuan, China. A similar outbreak was observed in another Chinese province in 1998. Symptoms reported in these two outbreaks include high fever, malaise, nausea and vomiting, followed by nervous symptoms, subcutaneous hemorrhage, septic shock and coma in severe cases. The increased severity of S. suis infections in humans, such as a shorter incubation time, more rapid disease progression and higher rate of mortality, underscores the critical need to better understand the factors associated with pathogenesis of S. suis infection. From the 35 capsular serotypes currently known, serotype 2 is considered the most virulent and frequently isolated in both swine and humans. Here, we review the epidemiological, clinical and immunopathological features of S. suis infection in humans.     

Evaluation of an enzyme-linked immunosorbent assay for detection of Eperythrozoon suis antibodies in swine.

An ELISA was developed and tested to detect antibodies to Eperythrozoon suis in swine. Results were compared with those of the indirect hemagglutination (IHA) test. Antigen isolated from swine heavily infected with E suis was used for both tests. Comparison of the ELISA with the IHA test revealed a significant (P less than 0.001) correlation between results. Of 114 samples obtained from 9 swine infected with E suis, 87.7% were seropositive (titer greater than or equal to 200) via the ELISA, and 80.7% were seropositive (titer greater than or equal to 20) via the IHA test. The sensitivity of the ELISA was greater than that of the IHA test. All blood samples obtained from specific-pathogen-free swine tested negative for E suis antibody. Cross-reactions were not observed between E suis antigen and antisera against various swine and cattle disease agents using ELISA. We concluded that the ELISA may be used for rapid and effective diagnosis of infection with E suis in swine.     

Streptococcus Suis: Past and Present

Staats, J.J., Feder, I., Okwumabua, O. and Chengappa, M.M., 1997. Streptococcus suis: past and present. Veterinary Research Communications, 21 (6), 381-407Steptococcus suis is a Gram-positive, facultatively anaerobic coccus that has been implicated as the cause of a wide range of clinical disease syndromes in swine and other domestic animals. In swine, the disease has spread worldwide but is more prevalent in countries with intensive swine management practices. The disease syndromes caused by S. suis in swine include arthritis, meningitis, pneumonia, septicaemia, endocarditis, polyserositis, abortions and abscesses. S. suis has also been implicated in disease in humans, especially among abattoir workers and swine and pork handlers. In humans, S. suis type 2 can cause meningitis, which may result in permanent hearing loss, septicaemia, endocarditis and death. The pathogenic mechanism of S. suis is not well defined. Several virulence factors have been identified, but their roles in pathogenesis and disease have not been well elucidated. Much work is in progress on characterization of virulence factors and mechanisms, with emphasis on the control of the disease. Because of the non-availability of suitable immunoprophylaxis, control of S. suis infection has depended mainly on the use of antimicrobials.     

人感染猪链球菌2型的研究进展

猪链球菌2型是一种重要的人畜共患病原菌,可引起人的脑膜炎、败血症等疾病.2005年6月发生的四川疫情是由猪链球菌2型引起的人猪共同感染,流行广,发展快,人猪患病数量多.本文从病原、流行特点、感染途径、临床表现、实验室鉴定(包括培养特性、形态特征、生化特性、血清凝集、毒力基因)等方面做了详细的综述,初步探讨了2型猪链球菌病的防治方法.     

The role of toll-like receptors in the pathogenesis of Streptococcus suis

Streptococcus suis is an important agent of swine and human meningitis. Sequence type (ST) 7 emerged in China and was responsible for the human epidemic caused by S. suis in 2005. The virulence of S. suis ST7 is greater than the wild type pathogenic S. suis, ST1; however, the mechanisms for this increased pathogenicity are unknown. The aim of this study was to determine the role of different toll-like receptors (TLRs) involved in regulating the host response to the S. suis infection and to speculate on differing mechanisms used by ST7 strains to induce disease. Here we compared two ST7 strains isolated in the 2005 Sichuan outbreak to two ST1 strains. Our data show TLR2, 6 and 9 are involved in the recognition of heat-killed S. suis independent of the ST type. We found the TLR-dependent cytokine production differed between the two types of strains using whole cell lysate proteins. TLR6 played a greater role in cytokine production induced by the whole cell lysate proteins from the ST7 strain than in that induced by the ST1 strain lysates. The data suggest that mechanisms of inflammation induced by S. suis strains differ where this will be useful in designing efficient strategies in combating streptococcal toxic shock-like syndrome caused by the S. suis ST7 strains.     

Experimental exposure of young pigs using a pathogenic strain of Streptococcus suis serotype 2 and evaluation of this method for disease prevention.

Control of Streptococcus suis infections and associated disease have proven to be a difficult challenge under most farm conditions. The objective of this study was to experimentally expose young pigs with a pathogenic strain of S. suis serotype 2 as a means of controlling the disease in a commercial swine farm. Prior to the start of the study, the pathogenic S. suis strain responsible for mortality in the farm was identified and used to experimentally inoculate baby piglets. Over a 3-week period, groups of pigs were selected (100 pigs/wk) and divided into 2 groups: control (50 pigs/week) and experimentally exposed (50 pigs/week). Pigs in the experimentally exposed group were inoculated at 5 d old by tonsillar swabbing with the pathogenic S. suis farm isolate. The effect of exposure with this pathogenic strain was evaluated during the nursery and finishing stages and was based on: morbidity (pigs with central nervous signs (CNS) and/or lameness), mortality and number of treatments required by pigs that had either CNS or lameness. The relative risk (RR) of acquiring disease due to S. suis infection was also calculated. Results showed that morbidity in the experimentally exposed groups was lower than in the control group and these results were statistically different (P = 0.006). Experimentally exposed pigs also showed a statistically significant reduction in lameness problems (P = 0.012), but not in CNS (P = 0.20) or mortality (P = 0.59). Pigs in the control group had an increased RR of 4.76, 8.77 and 2.7 for morbidity, to have lameness or to have CNS signs, respectively. In conclusion, experimental exposure of young pigs with the farm's pathogenic S. suis strain at a young age, had a positive effect in reducing clinical signs characteristics of S. suis infection. This method constitutes a novel approach to the control of S. suis infections in swine farms.     

猪致病性链球菌的分离鉴定

采集33例临床症状疑似猪链球菌感染猪的全血及内脏器官进行涂(触)片染色镜检,取有球菌感染的样品16份进行血琼脂平板分离纯化试验;根据血琼脂平板上菌落形态、溶血情况及对单个菌落涂片染色镜检结果,共筛选出12份可疑样品,进一步采用液体培养基对12份样品进行增菌及纯化培养;选取纯化培养菌进行链球菌生化试验,结果共鉴定出11株纯化的猪链球菌;11株猪链球菌均对小鼠表现出高致病力.     

猪丹毒的诊治

[目的]为查清湖南湘潭市3个猪场疫情发生的原因。[方法]开展了流行病学调查和临床诊断、病理解剖、实验室检测。[结果]本次疫情共有3个养殖场的32头猪发病,其中育肥猪、架子猪、仔猪分别发病3头、26头、3头,发病率分别为：37.5%、8.67%、11.5%;死亡4头,其中架子猪3头、仔猪1头,死亡率分别是：1.0%、仔猪3.85%。[结论]最终确诊这3个场/户的猪只发生了猪丹毒疫情,并提出了综合性防治措施。     

Recombinant DNA probe detecting Eperythrozoon suis in swine blood

A genomic library to Eperythrozoon suis DNA was constructed in lambda gt11, and from this library, E suis clone KSU-2 was identified as a potential diagnostic probe. In hybridization experiments that used 100-microliters samples of blood collected in chaotropic salt solutions, the KSU-2 probe hybridized strongly with purified E suis organisms and blood samples from splenectomized swine that were parasitized with E suis. However, the probe under stringent conditions did not give radiographic indications of hybridizing with equine blood DNA, bovine blood DNA infected with Anaplasma marginale, canine blood DNA infected with Ehrlichia canis, feline blood DNA infected with Haemobartonella felis, or uninfected swine blood DNA.     

临床健康猪群猪链球菌2型带菌率流行情况调查

为了解钦州市临床健康猪群中猪链球菌2型的流行情况,采用PCR方法对采集的病料进行检测分析。374份样品的PCR检测结果显示,链球菌、猪链球菌、猪链球菌2型的带菌率分别为81.8%、48.9%、2.9%,表明钦州市健康猪中猪链球菌带菌率高,但猪链球菌2型带菌率较低。