大西洋中部大眼金枪鱼垂直分布与温度、盐度的关系

根据2001年7月4日至10月27日3艘在大西洋中部公海海域作业的中国金枪鱼延绳钓船上随机观测到的大眼金枪鱼(Thunnus obesus)的上钩钩号,应用悬链线钩深计算公式,分别计算出各钩号的钩深;根据STD仪测得的温度、盐度的垂直分布以钩深为引数,查出该尾鱼捕获时的温度和盐度数据。根据大眼金枪鱼的取样数据,利用频度统计的方法,推算出各水层、水温、盐度范围的渔获率。渔获率最大的水层、水温、盐度范围为大眼金枪鱼的最适水层、水温和盐度范围;渔获率为前3位的水层、水温、盐度范围为大眼金枪鱼活动较频繁的水层、水温和盐度范围。结果表明：大西洋中部,大眼金枪鱼的最适水层为240．00～269．99m水深、最适水温为12．00～12．99℃、最适盐度为35．00～35．09;大西洋中部渔场大眼金枪鱼活动较频繁的水层为240．00～329．99m水深、水温为10．00～12．99℃、盐度为35．00～35．29。     

印度洋公海温跃层与黄鳍金枪鱼和大眼金枪鱼渔获率的关系

2005年9月15日-12月12日,金枪鱼延绳钓渔船"华远渔18、19号"对印度洋公海进行了金枪鱼渔业调查.利用多功能水质仪(XR-620)、温盐深仪(CTD SBE37SM)和微型温度深度计(TDR-2050)等获取海洋环境数据,得出作业海域的温跃层深度和强度,结合每天作业时记录的渔获数据,分别计算黄鳍金枪鱼和大眼金枪鱼在温跃层内和深水层的渔获率,研究温跃层与黄鳍金枪鱼和大眼金枪鱼渔获率之间的关系.结果表明:(1)2艘船分别有60.9%和60.0%的作业天数中,温跃层内黄鳍金枪鱼渔获率较高,"华远渔18号"船在温跃层内和温跃层以下的平均渔获率分别为18.22尾每千钩和6.04尾每千钩,"华远渔19号"船温跃层内和温跃层以下平均渔获率分别为2.22尾每千钩和1.31尾每千钩.通过t-检验成对双样本均值分析,温跃层以内和温跃层以下黄鳍金枪鱼总平均渔获率有显著性差异(P=0.02<0.05),温跃层以内比温跃层以下的渔获率明显要高;(2)2艘船分别有69.6%和100%的作业天数中,温跃层以下水深大眼金枪鱼渔获率较高,"华远渔18号"温跃层以内和温跃层以下平均渔获率分别为4.18尾每千钩和4.88尾每千钩."华远渔19号"温跃层以内和温跃层以下平均渔获率分别为0.10尾每千钩和2.57尾每千钩.大眼金枪鱼渔获率在温跃层以下水深较高.通过t-检验成对双样本均值分析,温跃层以内和温跃层以下大眼金枪鱼总平均渔获率无显著性差异(P=0.070.05),但对"华远渔19号"船的渔获率数据经t-检验发现大眼金枪鱼在温跃层以内和温跃层以下的渔获率有显著性差异(P=0.00<0.05).     

印度洋中南部水域金枪鱼延绳钓钓钩钩型对钓获对象的选择性

基于2008年9月至2009年4月在印度洋中南部水域金枪鱼延绳钓渔场收集的数据,研究分析和比较了3种钓钩钩型(传统金枪鱼钩、“J”形钩和圆形钩)的渔获效益及对钓捕对象的选择性。结果表明:(1) 从渔获种类上看,大眼金枪鱼和大青鲨金枪鱼钩钓获比例最高,“J”形钩和圆形钩的钓获比例相当;而长鳍金枪鱼则为金枪鱼钩钓获比例最高,其次为“J”形钩和圆形钩。(2) 大眼金枪鱼存活率以金枪鱼钩最高,“J”形钩最低;长鳍金枪鱼则为“J”形钩稍高于圆形钩,金枪鱼钩最低;大青鲨则以圆形钩最高,“J”形钩最低。(3) “J”形钩钓获的长鳍金枪鱼和鲨鱼平均叉长较金枪鱼钩和圆形钩稍大;而金枪鱼钩钓获的大眼金枪鱼平均叉长较圆形钩和“J”形钩稍大。(4) 3种钩型钓获的长鳍金枪鱼、大眼金枪鱼和大青鲨叉长分布均不存在显著性差异。     

大西洋中部金枪鱼延绳钓性能评估初步研究

利用2009～2010年我国大西洋中部金枪鱼延绳钓调查数据,对金枪鱼延绳钓钓具性能进行评估。结果表明:金枪鱼延绳钓具有较好的种类选择性,大眼金枪鱼渔获量和尾数分别占总渔获量的73.67%和76.00%;大眼金枪鱼(Thunnus obesus)、黄鳍金枪鱼(Thunnus albacares)和剑鱼(Xiphias gladius)未达到性成熟的渔获尾数比例分别为13.00%、25.97%和48.93%;1～6号钓钩,大眼金枪鱼上钩率随钓钩深度增加呈递增趋势,6～8号钓钩上钩率呈递减趋势,6号钓钩上钩率最大为9.46尾/千钩;3号至8号钓钩上钩率均大于7尾/千钩,表明1号和2号钓钩利用率偏低;根据钓钩理论深度,推测大眼金枪鱼主要分布水层为220m～350m。通过调节缩短率和浮子绳长度对钓具进行优化,使得钓钩分布水层与大眼金枪鱼分布水层更为接近,提高钓钩利用率。     

南海金枪鱼延绳钓作业参数优化

为了探索南海金枪鱼延绳钓合适作业参数,根据2010年6月至2013年2月8个航次的南海金枪鱼延绳钓探捕调查数据,估算了大眼金枪鱼和黄鳍金枪鱼在不同水层的渔获率,以浮子绳长度hf、两浮子间钩数n和短缩率k这3个作业参数的调整为研究对象,采用最小二乘法度量不同水层上钓钩分布频率与金枪鱼渔获分布频率的匹配程度。当两者之间的频差平方和达到最小值时,即认为找到延绳钓最合适作业结构。结果显示:hf=34 m、n=25、k=0.68°对捕捞大眼金枪鱼最合适;hf=10 m、n=14、k=0.71°对捕捞黄鳍金枪鱼最合适;两种兼顾时,hf=8 m、n=27、k=0.69°更合适。     

吉尔伯特群岛海域延绳钓渔场大眼金枪鱼的环境偏好

为了掌握基里巴斯吉尔伯特群岛附近海域大眼金枪鱼的环境偏好,2009年9月至12月,金枪鱼延绳钓船"深联成719"在该海域进行了调查。利用仪器获取海洋环境数据,结合每天渔获数据,应用逐步回归方法,建立钓钩深度预测模型,计算大眼金枪鱼在各水层、温度、盐度、叶绿素、含氧量、水平海流和垂直海流范围内的渔获率,渔获率最大的各环境因子范围为大眼金枪鱼偏好的环境。结果表明:(1)大眼金枪鱼偏好的水层、水温、盐度、叶绿素、含氧量、水平海流和垂直海流范围分别为200.0~240.0 m、14.0~15.0℃、35.00~35.10、0.24~0.26μg/L、3.0~4.0 mg/L、0.00~0.20 m/s和0.03~0.04 m/s;(2)一般情况下,接近成熟的大眼金枪鱼偏好的水温为14.0~17.0℃;(3)大眼金枪鱼的适盐性较广;(4)溶解氧高于门限值(0.8 mg/L)时,大眼金枪鱼的分布由其它环境因子决定。     

印度洋大眼金枪鱼延绳钓适宜渔获环境的初步研究

数值分析得出大眼金枪鱼的渔获适宜环境参数值范围：温度14～17℃,盐度34．5～35．4．溶解氧浓度1．5～4．5mg／L,温跃层深度80～160m,营养盐氮、磷、硅浓度分别大于12-μg／L,09μg／L,14μg／L。聚类分析表明,钓获率与营养盐关系最为密切。另外,通过因子分析,深化了对钓获率和渔获环境因子两者间关系的理解。     

金枪鱼延绳钓环形钩和圆形钩钓获率比较

根据"华远渔18号"和"华远渔19号"两艘冰鲜金枪鱼延绳钓渔船2005年9月15日~12月12日在印度洋热带公海水域(0°47′~10°16′N,61°40′~70°40′E)作业期间收集的数据,以每千钩钓获尾数和平均净重,对环型钩与圆型钩对大眼金枪鱼和黄鳍金枪鱼的选择性、钓获率差异等进行了比较研究。结果表明:不同钓具漂移速度下环型钩与圆型钩各自对大眼金枪鱼和黄鳍金枪鱼的选择性无显著差异;不同钓具漂移速度下环型钩与圆型钩之间对大眼金枪鱼和黄鳍金枪鱼的钓获率无显著差异;环型钩与圆型钩之间对大眼金枪鱼和黄鳍金枪鱼渔获物净重无显著差异。     

北太平洋金枪鱼延绳钓钓钩垂直分布及浸泡时间对渔获物的影响

根据中国金枪鱼延绳钓渔业观察员于2018年9-12月在北太平洋公海(150°W-164°W,30°N-37°N)采集的金枪鱼延绳钓渔获数据,分析了钓钩的垂直分布及浸泡时间与渔获物之间的关系。结果表明,11种主要渔获物钓获深度差异很大,鲣(Katsuwonus pelamis)的平均钓获深度最浅,大青鲨(Prionace glauca)最深;除大青鲨和帆蜥鱼(Alepisaurus ferox)外,其他兼捕鱼类的钓钩深度分布与大眼金枪鱼(Thunnus obesus,目标鱼种)之间差异显著;随着浸泡时间的增加,总渔获率呈先增加后下降的趋势,10~14 h内渔获率较高,且在第10小时达到峰值[1.44尾·(千钩·h)-1];浸泡时间在10~16 h内大眼金枪鱼的渔获率较高,且在第11小时达到峰值[0.45尾·(千钩·h)-1]。研究结果可为渔业生产和CPUE的标椎化提供参考,为制定有效减少兼捕鱼种的管理措施提供科学依据。     

罩网兼作金枪鱼延绳钓的钓钩深度与渔获水层分析

2015年3-4月及2017年1月在南海外海进行了两次罩网渔船兼作金枪鱼延绳钓的捕捞试验,基于试验数据分析延绳钓钓钩深度及渔获水层分布,以期优化改进试验钓具,有效提高南海金枪鱼延绳钓捕捞效益。研究发现:1)经济渔获以剑鱼(Xiphias gladius)与黄鳍金枪鱼(Thunnus albacares)为主,剑鱼的单位捕捞努力量渔获量(CPUE,kg·千钩-1)为所有渔获中最高; 2)钓钩深度(D)与钩位(n)、风速(SW)、风流合压角(γ)成正相关,与漂移速度(SD)、投绳船速(SV)呈负相关,回归方程为:D=10. 259n-37. 247SD-29. 878SV+6. 940SW+23. 493γ+35. 633; 3)模型的预测结果与实测数据间无显著性差异,相对权重表明各影响因子中钩位具有最大的相对重要性; 4)试验中拟合钩深分布范围为35. 08～110. 80 m,剑鱼上钩率与CPUE最高的水层为60～80 m,黄鳍金枪鱼上钩率与CPUE最高的水层为80～100 m; 5) 60～80 m水层主要经济渔获CPUE最大且钓钩数目最多,认为钓钩水层分布较合理。     

Association between the interannual variation in the oceanic environment and catch rates of bigeye tuna (Thunnus obesus) in the Atlantic Ocean

The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna.     

Rare serotype occurrence and PFGE genotypic diversity of Streptococcus agalactiae isolated from tilapia in China

Previously, we reported 10 PEGE types of 85 tilapia Streptococcus agalactiae(GBS), which shifted from Streptococcus iniae in China, by using PEGE method. Presently, larger and more representative tilapia GBS were isolated, for the ?rst time in China, to characterize their serotypes and genetic diversities more precisely than had done before. 168 GBS strains were distributed in ?ve provinces of China, in which Guangdong, Guangxi and Hainan were the major ones, holding36.9%(62/168), 37.5%(63/168) and 19.6%(33/168), respectively. Serotypes, Ia, Ib and III, were observed in these strains and the most predominant one was Ia(95.2%), which mainly distributed in Guangdong, Guangxi and Hainan. Ia initially occurred in 2009, it shoot up to 32.1% in 2010,but decreased to 16.1% in 2011 before went up to 45.2% in 2012. Ib sporadically occurred during2007–2011, III onlyoccurred in 2012. 14 different PFGE types, including 4 new types(N, O,P and Q), were observed, in which B, D, F and G were the predominant types, holding 83.9%(141/168) of the total GBS strains. Ia corresponded to 11 PFGE types(A–H, N–P), in which type D predominated(51%). Ib represented 3 genotypes(I, J and Q) and III harbored only 2genotypes(N and F). Type N and Fsynchronously presented in Ia and III. In summary, the genetic diversity of tilapia GBS varied by serotypes and changed with geographical locations and years.Although Iastillpredominated, new rareserotypeIII alreadyoccurred in China.     

Evaluation of a Fast Method Based on the Presence of Two Restriction Sites in the Mitochondrial ND5 (mt ND5) Gene for the Identification of Scomber Species

The purpose of this work was to evaluate the suitability of a method based on the presence of two restriction sites (for Hae III and Hindf I) in the mitochondrial NADH dehydrogenase subunit 5 (mt ND5) gene to identify Scomber species. The evaluation was performed on 144 reference and market samples by sequencing of the entire 505-bp fragment of the mt ND5 gene and of a 464-bp fragment of the Kocher fragment of the cytochrome b gene (mt Cytb). Sequence analysis of any of the two fragments allows the identification of each of the four Scomber species, but S. japonicus and S. colias had the same restriction sites at the ND5 amplicon and would not have been differentiated by this analysis. Similarly, loss of the Hae III site in some S. scombrus individuals would have misidentified them as not being Scomber. All the market products were correctly labeled except one acquired in Spain labeled as originating in the Atlantic and containing S. japonicus.     

Growth performance,digestive enzyme activity and immune response of Japanese sea bass,Lateolabrax japonicus fed with fructooligosaccharide

In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg.     

Purification and partial characterization of GtHs (cfLH and cfFSH) from Indian walking catfish (Clarias batrachus) (L.) and development of a homologous ELISA for cfLH

Two gonadotropins (GtH; Qa and Qb) were purified by gel filtration and ion exchange chromatography from the pituitaries of Indian walking catfish (Clarias batrachus). The presence of GtH during purification was assessed by in vitro oocyte maturation and in vivo steroidogenic activity, and their identities were determined by elution profiles, molecular weight, biological activities and yield. The molecular weights of Qa and Qb were 37 and 42 kDa, respectively, and composed of distinct subunits (Qa: 20 and 14 kDa and Qb: 26 and 18 kDa). Polyclonal antibodies raised against Qa immunostained Qa, Qb and pituitary GtH cells. A competitive Qa‐ELISA was developed whose sensitivity was 6.25 ng mL^?1 (1.25 ng well^?1) with intra‐ (3.5%) and inter‐ (12.4%) assay coefficients of variation. Displacement curves parallel to the standard were obtained with plasma and pituitary extracts of catfish, Qb and carp GtHII. The assay was validated by measuring the plasma Qa levels after LHRH treatment and in relation to ovarian growth in the female catfish during different reproductive phases. Based on the results, Qa and Qb corresponded to fish LH and FSH respectively. The findings will increase the knowledge of the mechanisms controlling fish reproduction and identification of sensitive phases in fish in captivity for hormonal manipulation.     

Controlled infection of Poecilia reticulata Peters (guppy) with Tetrahymena by immersion and intraperitoneal injection

Tetrahymena is a protozoan parasite, which infects guppy, Poecilia reticulata Peters, and causes substantial economical losses in commercial farms worldwide. Studies of guppy infected by Tetrahymena require standardized infection protocols. The LD50 for Tetrahymena infection of guppies by intraperitoneal (IP) injection was calibrated, and the level obtained was 946 parasites per fish. Guppy infection with Tetrahymena by immersion, imitating the natural route of infection via the integument, was studied under normal or stress conditions. Exposure to cold and netting (CNI) and to cold only (CI) followed by immersion exposure to 10 000 Tetrahymena per mL resulted in 22.5% and 19.2% mortality, respectively, as compared to 14.2% and 10% in groups that were netted only (NI) or non‐stressed (I). Histopathology revealed that immersion infection resulted in a systemic infection. Lysozyme levels, measured 3 weeks after infection, were significantly higher in the CNI group (288 μg per mg protein) compared with CI‐, NI‐ and I‐treated groups (94.5, 64 and 62.3 μg mg^?1, respectively). There was no evident parasite immobilization activity in body homogenates, suggesting no development of acquired immunity. Re‐infection by IP injection revealed no increase in protection in any of the treatment groups, mortality range of 56.3–75%, higher than in the non‐exposed control (40.6% mortality).     

The optimal dietary protein level of juvenile silver sillago Sillago sihama at three dietary lipid levels

A 5 × 3 factorial growth trial was conducted to evaluate optimal dietary protein and lipid levels (dietary protein level, DP; dietary lipid level, DL) for juvenile Sillago sihama (S. sihama) (2.0 ± 0.02 g, initial weight). Fish were fed 15 diets containing 5 DPs (350, 400, 450, 500 and 550 g/kg) and 3 DLs (60, 90 and 120 g/kg) for 8 weeks. The interaction between proteins and lipids significantly influenced the feed conversion ratio, condition factor, body composition, antioxidant indices and lipase activity (p < .05). DP 450 g/kg showed the highest average final body weight. DPs 500 and 550 g/kg significantly decreased the protein efficiency ratio (p < .05). DL 120 g/kg showed the highest percentage weight gain. The low feed conversion ratio was found in diets P45L12, P55L9 and P55L12. Diet P45L12 showed high superoxide dismutase activities. DP 450 g/kg showed the lowest average malondialdehyde content. Lipase activity was increased by increasing DP (p < .05) with a fall at DP 550 g/kg. Under the present experimental conditions, the optimal DP for S. sihama was 450 g/kg under the DL 120 g/kg.     

Vitamin D3 and the renal handling of phosphate in American eels

Cholecalciferol (Vitamin D₃) increased plasma inorganic phosphate concentration in American eels,Anguilla rostrata, in a dose-dependent fashion. This response was more marked in phosphate loaded fish. In control as well as phosphate loaded eels the hyperphosphatemic response to D₃ was associated with a sharp reduction in renal phosphate clearance relative to¹⁴C-polyethelene glycol (PEG) clearance. Glomerular filtration and urine flow rates were not affected by D₃. As renal phosphate clearance, even in phosphate loaded eels, never significantly exceeded that of PEG, it is suggested that D₃ reduced the relative clearance rate of phosphate by increasing renal phosphate reabsorption rather than by reducing the tubular secretion of phosphate.