Structure-Activity Relationships (SAR) studies of benzoxazinones, their degradation products and analogues. phytotoxicity on standard target species (STS)

Benzoxazinones 2,4-dihydroxy-7-methoxy-(2H)-1,4-benzoxazin-3(4H)-one (DIMBOA) and 2,4-dihydroxy-(2H)-1,4-benzoxazin-3(4H)-one (DIBOA) have been considered key compounds for understanding allelopathic phenomena in Gramineae crop plants such as corn (Zea mays L.), wheat (Triticum aestivum L.), and rye (Secale cereale L.). The degradation processes in the environment observed for these compounds, in which soil microbes are directly involved, could affect potential allelopathic activity of these plants. We present in this work a complete structure-activity relationships study based on the phytotoxic effects observed for DIMBOA, DIBOA, and their main degradation products, in addition to several synthetic analogues of them. Their effects were evaluated on standard target species (STS), which include Triticum aestivum L. (wheat) and Allium cepa L. (onion) as monocots and Lepidium sativum L. (cress), Lactuca sativa L. (lettuce), and Lycopersicon esculentum Will. (tomato) as dicots. This permitted us to elucidate their ecological role and to propose new herbicide models based on their structures. The best phytotoxicity results were shown by the degradation chemical 2-aminophenoxazin-3-one (APO) and several 2-deoxy derivatives of natural benzoxazinones, including 4-acetoxy-(2H)-1,4-benzoxazin-3(4H)-one (ABOA), 4-hydroxy-(2H)-1,4-benzoxazin-3(4H)-one (D-DIBOA), and 4-hydroxy-7-methoxy-(2H)-1,4-benzoxazin-3(4H)-one (D-DIMBOA). They showed high inhibitory activity over almost all species growth. The fact that APO is a degradation product from DIBOA with high phytotoxicity and stability makes it possible to assign an important ecological role regarding plant defense mechanisms. 2-Deoxy derivatives of natural benzoxazinones display a wide range of activities that allow proposing them as new leads for natural herbicide models with a 1,4-benzoxazine skeleton.     

Fate of benzoxazinone allelochemicals in soil after incorporation of wheat and rye sprouts

Growing cereals (especially rye), which are incorporated into the soil to increase soil fertility or organic matter content, is a common practice in crop rotation. The additional sanitizing effect of this incorporation has often been appreciated and is said to be due to leaching of benzoxazinones and subsequent formation of benzoxazolinones. In this study wheat (Stakado) and rye (Hacada) sprouts were incorporated into soil in amounts that simulated agricultural practice. By extraction and subsequent LC-MS analysis the disappearance and appearance of benzoxazinones, benzoxazolinones, and phenoxazinones in soil were followed. In the wheat experiments 6-methoxybenzoxazolin-2-one (MBOA) was detected as the main compound. 2-Hydroxy-7-methoxy-1,4-benzoxazin-3-one (HMBOA) and 2-hydroxy-1,4-benzoxazin-3-one (HBOA) were detected as well. No phenoxazinones were detected. For the rye experiment the picture was more complex. In the first 2 days of incubation MBOA and 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA) were detected as the main allelochemicals along with HBOA, HMBOA, and benzoxazolin-2-one (BOA), in decreasing order. Later in the incubation period some 2-amino-3H-phenoxazin-3-one (APO) was detected and the amount of HBOA increased considerably and decreased again. The profiling of the benzoxazinone metabolites and their derivates in soil was dynamic and time-dependent. The highest concentrations of most of the compounds were seen at day 1 after incorporation. A maximum concentration was reached at day 4 for a few of the compounds. This study is the first of its kind that shows the dynamic pattern of biologically active benzoxazinone derivates in soil after incorporation of wheat and rye sprouts. Methods for organic synthesis of HBOA and HMBOA were developed as part of the study.     

Genomics as a Tool for Cereal Chemistry

Genomics is providing a new set of tools for cereal chemistry. Analysis of the DNA of the major cereals is increasing the understanding of the basis of grain quality at the gene level. Differences in the sequence of the gene in different cultivars or differences in the level of expression of the gene may be used to explain differences in processing or end-use quality characteristics. For example, quantitative analysis of the levels of expression of genes at different stages during seed development in wheat or germination in barley can be used to define the genetic basis of differences in wheat and barley quality. Rice quality traits such as fragrance and gelatinization temperature (cooking temperature) can be explained by DNA sequence differences in specific genes identified using genomics approaches. Rapid and reliable species and cultivar identification based on DNA analysis methods developed using genomics tools can be applied to grain and to food products. This technology has special advantages in the analysis of complex mixtures of cereals. Technologies for very high-throughput and very low-cost analysis of large numbers of samples are now available. These may be applied by cereal chemists at many levels: selection in cereal breeding, optimizing processing, and analysis of the identity and composition of grain or food samples.     

Modified benzoxazinones in the system Oryza sativa-Echinochloa crus-galli: an approach to the development of biorational herbicide models

The utility of benzoxazinones and some of their synthetic derivatives in the search for new leads for herbicide model development has been explored. The work described focuses on obtaining derivatives that present selectivity in the system Oryza sativa- Echinochloa crus-galli. To achieve this goal the influence of lipophilicity in this system has been studied by preparing 14 ester derivatives at the N-4 position of D-DIBOA along with other compounds with different functionalization and chain lengths at position C-2. These compounds have been tested in the aforementioned system, and the dose-response profiles have been compared. The most active compound was 2-ethyl-4-hydroxy-(2H)-1,4-benzoxazin-3(4H)-one, which presented higher selectivity than the specific herbicide Cotanil-35. These results confirm the potential of D-DIBOA as a lead herbicide for the control of Echinochloa spp. in rice crops.     

Structure-activity relationship studies of benzoxazinones and related compounds. Phytotoxicity on Echinochloa crus-galli (L.) P. Beauv

Echinochloa crus-galli (E. crus-galli; barnyardgrass) is a weed widely distributed. It constitutes a serious weed problem in 42 countries and has been found in at least 27 more. It is the world's main weed of rice affecting up to 36 crops worldwide. Several biotypes of this plant, with resistance to herbicides with different modes of action have evolved. In our ongoing studies regarding the potential application of benzoxazinones and their soil degradation products for weed control, a complete structure-activity relationships (SARs) study was made by using barnyardgrass as the target plant. Compounds used in this study were previously tested on a wide variety of standard target species (STS), and they include natural allelochemicals 2-O-beta-D-glucopyranosyl-4-hydroxy-(2H)-1,4-benzoxazin-3(4H)-one (DIBOA-Glc), 2,4-dihydroxy-7-methoxy-(2H)-1,4-benzoxazin-3(4H)-one (DIMBOA), and 2,4-dihydroxy-(2H)-1,4-benzoxazin-3(4H)-one (DIBOA), together with some degradation derivatives found in wheat crop soil and some synthetic analogues. Their phytotoxicity on E. crus-galli is discussed and compared with the results obtained from previous screening. This work constitutes the next step in the search for natural herbicide models based on benzoxazinones and their degradation products. The most active compounds were the degradation product 2-aminophenol (APH) and the synthetic analogue 4-hydroxy-(2H)-1,4-benzoxazin-3(4H)-one (D-DIBOA). Their activities confirm the direction proposed in our previous SAR study, which establishes D-DIBOA to be the best lead for natural herbicide model development with benzoxazinone structure.     

泡桐、杨树叶水浸液对作物种子萌发的化感作用

为了研究农林复合生态系统中树种对农作物种子萌发的影响,以泡桐和杨树为对象,采用振荡溶解的方法提取泡桐和杨树叶浸出液,通过测定种子萌发率,研究树叶水提取物对小麦、玉米、大豆种子萌发的化感作用。结果表明：（1）杨树和泡桐叶水提取物质量浓度达到50 mg/mL对大豆的发芽率、发芽速率有明显抑制作用;杨树叶水提取物质量浓度达到10 mg/mL和泡桐叶水提取物质量浓度高于20mg/mL对小麦、玉米有显著的抑制作用。（2）杨树叶水浸液对3种作物种子萌发的化感作用（RI=15.147）大于泡桐叶（RI=13.973）。（3）杨树和泡桐叶水浸液对3种作物种子的化感抑制作用强度依次为小麦>玉米>大豆,而且随着杨树和泡桐叶水浸液质量浓度的上升其化感抑制作用越来越强,作物种子的发芽率也越来越低。     

田间旱育条件下不同化感潜力水稻根际土壤酚酸类和萜类物质分析

酚酸类和萜类是水稻化感作用研究中研究较多、争议较大的2大类化感物质,但目前有关水稻根系分泌物的研究多在实验室条件下进行。本文以国际公认的强化感水稻‘PI312777’和弱化感水稻‘Lemont’为材料,以未种植水稻的土壤为对照,研究了其在田间旱育条件下,不同土壤水分状态(旱地和湿地)时,根际土壤酚酸类和萜类物质的差异。结果表明,不同水分条件下不同化感潜力水稻品种和对照根际土壤中酚酸类物质和萜类物质的组成较为相似,但各物质含量存在一定差异。适度旱胁迫下,各处理根际土壤中所检测到的咖啡酸、对羟基苯甲酸、香草酸、阿魏酸和肉桂酸5种酚酸类物质及总量均有提高,其中强化感水稻‘PI312777’根际土壤中5种酚酸类物质提高最显著,比CK湿地处理提高2.84倍;在各处理根际土壤共检测到的27种萜类物质中,17种是含氧单萜;干旱胁迫导致各处理根际土壤单萜烯、含氧单萜、含氧倍半萜和总萜变化程度和变化趋势不同,单萜烯相对含量在强化感水稻‘PI312777’根际土壤中明显提高,而在弱化感水稻‘Lemont’和对照根际土壤中则降低。本文在此基础上讨论了由此导致2种水稻田间化感抑草效果差异的原因与机制。     

Concentration of benzoxazinoids in roots of field-grown wheat (Triticum aestivum L.) varieties

Benzoxazinones are naturally occurring secondary metabolites of some Gramineae plants, responsible for their resistance to some pathogenic fungi and for their allelopathic action. Six varieties of winter wheat grown in fields under organic or conventional systems and 11 old accessions were tested for two consecutive seasons and three plant development stages for the concentration in their roots of cyclic hydroxamic acids and their degradation products. This is the first report of six benzoxazinones analyzed in plants grown in the field. An analytical technique employing LC-DAD was used for determination. It was shown that 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one, its degradation product 6-methoxybenzoxazolin-2-one, and the lactam 2-hydroxy-7-methoxy-1,4-benzoxazin-2-one were predominant compounds in all tested samples. Their concentrations significantly differed with plant development stage and season, but no significant differences were found between varieties and between plant cultivation systems. The concentrations of 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA) and its degradation product benzoxazolin-2-one (BOA) were much lower, ranging from 60 to 430 mg/kg of dry matter, depending on accession, stage of development, and season. There was no significant difference found between plants grown in different cultivation systems, but there were significant differences between old and new varieties; concentrations of DIBOA and its derivatives were significantly lower in old accessions. It was concluded that the concentrations of DIBOA and BOA, which are precursors of highly fungicidal 2-aminophenol, 2-amino-3H-phenoxazin-3-one, and 2-acetylamino-3H-phenoxazin-3-one, are theoretically high enough to protect plants against some soilborne pathogens.     

苜蓿根际土壤化感潜力的初步研究

通过盆栽试验和发芽实验初步研究了4个生长年限(处理)的苜蓿地土壤化感作用强弱,其抑制活性强弱依次为:三年生苜蓿地>二年生苜蓿地>一年生苜蓿地>休闲地;在相同处理条件下,苜蓿对其他作物的化感作用强于其自毒效应。在一定年限内,苜蓿化感物质通过根系分泌、残茬分解和地上部分淋溶等途径进入土壤中,呈现出逐年富集的趋势。     

Autumn-sowing of cereals reduces breeding bird numbers in a heterogeneous agricultural landscape

The switch from spring-sown to autumn-sown cereals and the loss of habitat heterogeneity are often suggested to be key drivers of breeding bird decline on arable farmland. Yet, both factors are interlinked and it remains uncertain whether autumn-sown cereals reduce breeding bird numbers also in the structurally complex arable farmland of northern Europe. We tested whether autumn-sowing of cereals at both local and landscape scales affected the breeding bird community in a heterogeneous agricultural landscape of south-central Sweden. Rotation between sowing types was used as a semi-experiment based on 34 spring- vs. 41 autumn-sown cereal plots centred on infield non-crop islands of similar structure, size and surroundings. Species richness and territory abundance of ground-foraging species were significantly lower in autumn- than in spring-sown cereal plots both in the crop fields and the infield non-crop islands during the breeding season. No such effect was observed among foliage gleaning birds. Species richness in spring-sown cereal plots was less the more autumn-sown crops in the surrounding landscape within a 500 m radius. Average skylark densities did not differ between autumn- and spring-sown cereal plots because habitat preferences changed; densities declined in autumn-sown cereals during the growing season whereas they increased on spring-sown fields which had shorter swards throughout the breeding season. Our results indicate that negative effects of autumn-sown crops on breeding bird numbers spill over into both neighbouring non-crop and crop habitats. We conclude that agri-environmental schemes should place more emphasis on facilitating the value of the cropped area of fields as a foraging and nesting habitat. The retention of various non-crop habitats alone may not provide sufficient food close to nest sites for farmland birds that rely on crop fields for foraging.     

黄土高原典型区退耕还林还草工程实施效果实证分析

为缓解黄土高原地区水土流失、土地退化等生态环境问题,中国自1999年开始在该地区实施退耕还林还草等生态保育工程。选取甘肃省会宁县为研究区,基于农户问卷调查、统计资料及相关政府报告,应用统计学软件SPSS和满意度指数分析法,对典型区退耕还林还草工程实施前后土地利用、种植养殖结构、农户经济收入的变化进行了研究,并调查了农牧民对退耕还林还草工程影响的满意度,旨在评估退耕还林还草工程实施对黄土高原典型区农户生产生活的影响。结果显示,退耕还林还草工程对会宁县的土地利用结构具有重要影响,有4.48%的耕地转换成草地和林地,退耕还林还草工程取得了较好效果。但与此同时,农户问卷调查显示,有0.08 hm2·户-1大于25°坡耕地未实施退耕,表明工程实施需要加强监督并及时巩固退耕成果。工程实施后,会宁县谷物、薯类、豆类和油料作物的种植面积和单位面积产量都有一定增长,产量提高的主要原因是耕地的投入和耕作集约化程度有所提高。由于耕地面积减少,为提高土地利用效率,会宁县政府通过重点发展地膜玉米产业调整种植业结构,玉米种植面积增加近3倍。过去10多年间,会宁县还修建了大量梯田,这也有利于提高单位土地面积产出。另外,退耕还林工程实施后,会宁县家畜养殖数量有一定增长,但由于自然环境限制和饲草来源不足等原因,养殖规模维持在较低水平。由于连年旱灾导致种植业风险增大,农户环保意识增强,打工收入高等原因,较多农户选择外出打工,使打工收入成为最重要的收入来源,占总收入的62.69%。农户对退耕还林还草工程实施的9个方面较为满意,但在就业和收入方面满意度较低,满意度分别为-0.1和-0.2。本研究结果可为黄土高原典型区退耕还林还草工程进一步实施和生态系统可持续管理决策提供参考。     

Drought risk to agricultural land in Northeast and Central Germany

Aim of the study was the evaluation of the present state and further development of drought risk to agricultural sites in Northeast (NE) and Central Germany in consideration of climate changes. Based on the Medium Scale Agricultural Site Map, soil‐hydrological data were derived for heterogeneous soil areas. They refer to the landscapes in NE and Central Germany characterized by low precipitation, marked spatial soil heterogeneity, and a high share of hydromorphic soils. The soil data were linked with long‐term climate records of 368 stations for the period 1951–2000 as well as a climate scenario for the period 2001–2055. The plant water supply was calculated for three crop groups: cereals, root crops, and grass. The current and future development of drought risk of agricultural land of NE and Central Germany was evaluated. Starting in 1951, the water supply over the vegetation period has been decreasing with time for all crop groups up to now and may continue up to the year 2055 at most sites. However, there are also regions with increased plant water supply. The federal states of Brandenburg and Saxony‐Anhalt showed the strongest water deficit. Especially in these states, the plant water supply is strongly limited for cereals already today and probably may get worse for all crops in the future. On an average of the years, drought may limit plant growth in parts at >40% of agricultural land. In the federal states Mecklenburg‐Western Pomerania, Saxony, and Thuringia, the plant water supply mostly will stay in an approximately sufficient range due to higher precipitation and more favorable soil quality. The results are a background for the assessment of land‐use planning and evaluation of current and future soil‐ and site‐specific crop growing suitability on a medium scale.     

Role of Cover Crops in Improving Soil and Row Crop Productivity

Abstract

Cover crops play an important role in improving productivity of subsequent row crops by improving soil physical, chemical, and biological properties. The objective of this article is to review recent advances in cover crops practice, in the context of potential benefits and drawbacks for annual crop production and sustained soil quality. Desirable attributes of a cover crop are the ability to establish rapidly under less than ideal conditions, provide sufficient dry matter or soil cover, fix atmospheric nitrogen (N), establish a deep root system to facilitate nutrient uptake from lower soil depths, produce organic matter with low‐residue carbon/nitrogen (C/N) ratio, and absence of phytoxic or allelopathic effects on subsequent crops. Cover crops can be leguminous or nonleguminous. Leguminous cover crops provide a substantial amount of biologically fixed N to the primary crop, as well as ease of decomposition due to their low C/N ratio. Legume cover crops also possess a strong ability to absorb low available nutrients in the soil profile and can help in increasing concentration of plant nutrients in the surface layers of soil. Some nonleguminous cover crops having high N scavenger capacity compared with leguminous crops and sometimes, the growth of these scavenging grass cover crops is limited by N deficiency, growing grass/legume mixtures appears to be the best strategy in obtaining maximum benefits from cover crops.     

Pea-oat intercrops are superior to pea-wheat and pea-barley intercrops

Abstract

A three-year field experiment was conducted in Estonia to determine which combinations of pea (Pisum sativum L.) and wheat (Triticum aestivum L.), oats (Avena sativa L.) and barley (Hordeum distichon L.) were most suitable for mixed cultivation and the effect of pea on the yield potential of cereals. The inclusion of pea in cereal seeds and the increasing of its seed density led to substantial decreases in the grain yields of the cereal component. The reason for these decreases was the formation of smaller grains in cereals when intercropped with pea. The inclusion of pea in a cereal crop and the increasing of its seed density led to substantial increases in the protein content of the cereal grains. In barley and oats the increases in grain protein content were the lowest of the three cereals. At the same time, the maximum protein yield per area unit in cereals was obtained from plots of pure crops. In a mix with pea, the amounts of nitrogen consumed by cereals decreased and the protein yield of cereals per area unit were reduced in intercrops. Pea-cereal mixes had an advantage over cereal sole crops with regard to protein yield, due to the pea component. Pea-cereal mixes are particularly suitable for the conditions of organic farming, and should be recommended to farmers, as they ensure a relatively good harvest and high protein yield on soil without nitrogen fertilizers. In conclusion, the study showed that, of the three combinations, pea-oat mixed intercrops gave the highest yield of grain and protein yields.     

Essential oils from Mediterranean lamiaceae as weed germination inhibitors

The essential oils obtained from rosemary (Rosmarinus officinalis L.), thyme (Thymus vulgaris L.), and savory (Satureja montana L.) and the four monoterpenes that are their major constituents have been analyzed by GC and GC-MS and tested for their allelopathic properties on the seeds of three different annual weeds (Chenopodium album, Portulaca oleracea, and Echinochloa crus-galli) and three crops (Raphanus sativus, Capsicum annuum, and Lactuca sativa), with the aim to evaluate in vitro their potential as germination inhibitors. The essential oil composition varied with the species, thymol being the main constituent (44%) of thyme and carvacrol (57%) that of savory oil. Differences in essential oil composition were observed within two different rosemary ecotypes, type A, with alpha-pinene (37%) and 1,8-cineole (23%), and type B, characterized by a 2-fold content of 1,8-cineole (47%). This latest essential oil inhibited completely the germination of weeds while concurrently displaying little effect on pepper. The other two oils showed less selective action. S. montana essential oil, with 57% carvacrol, is the most active compound, completely inhibiting germination both of crops and weeds. Borneol, one of the main constituents of the oil of rosemary type B, showed an activity comparable to that of the whole oil. Crop and weed seeds treated with 1,8-cineole showed germination values that were not significantly different from controls, even if a slowing of the germination process expressed in terms of a significant increase in mean germination time was observed. Monoterpene compounds also present in the essential oils mainly represented the volatile fraction released from the crops and their residues into the soil.     

1992-2017年中亚五国农作物水足迹变化特征

本研究以中亚五国为研究区域,以农作物水足迹为评估手段,分析了1992—2017年间中亚五国农作物水足迹的时空变化特征,以揭示中亚五国农作物水足迹结构,厘清引起中亚五国水足迹变化的主要农作物的贡献。结果表明:1)中亚五国农作物水足迹时间变化特征明显,1992—2017年中亚五国农作物绿水足迹(-9.7×10~9 m~3)和蓝水足迹(-5.6×10~9 m~3)均趋于减少。中亚五国农作物水足迹空间分布差异显著,哈萨克斯坦农作物绿色水足迹最高(平均4.96×10~(10) m~3),且远高于其他4国(平均3.6×10~9 m~3),而蓝水足迹以乌兹别克斯坦最高(平均1.53×10~(10) m~3)。农作物绿水足迹和蓝水足迹增长率最大的国家分别是土库曼斯坦(87.6%)和吉尔吉斯斯坦(32.3%);绿、蓝水足迹减少率最大的国家分别是哈萨克斯坦(-20.7%)和乌兹别克斯坦(-24.2%)。2)中亚地区农作物绿水足迹主要以粮食作物为主,蓝水足迹以粮食作物和油料纤维作物为主;而粮食作物水足迹结构中,主要以小麦、水稻和玉米为主,油料纤维作物水足迹结构中,主要以棉花为主。3)哈萨克斯坦作为绿水足迹减少最多的国家,大麦(51.6%)和小麦(28.2%)的贡献最大;乌兹别克斯坦作为蓝水足迹减少最多的国家,棉花(61.9%)贡献最大。通过开展中亚地区的农作物水足迹研究,发现中亚农作物水足迹整体呈下降趋势,厘清了中亚地区引起水足迹下降的主要农作物种类,相关成果可以为中亚地区的农作物优化种植和水资源节约提供支撑。     

Tillage intensity effects on soil properties and crop yields in a long-term trial on morainic loam soil in southeast Norway

Many tillage studies focus primarily on grain crops, whereas other important agricultural crops receive little attention. This paper presents yield results for various crops grown in the tenth to sixteenth year of a long-term tillage trial on loam soil in southeast Norway. Traditional plough tillage was compared with deep and shallow tine cultivation and with minimum tillage, and the residual effects of tillage were measured in the seventeenth and eighteenth years. Soil bulk density, air permeability and other soil conditions were found to be favourable for crop growth on all treatments in the tenth year. The yield levels of cereals (Hordeum vulgare L., Triticum aestivum L. and Avena sativa L.) and potatoes (Solanum tuberosum L.) showed consistent increases of 2–8% with declining tillage intensity, whereas yields of fodder beet (Beta vulgaris L.) were highest after plough tillage. The latter result was thought to be due to lower soil temperatures under reduced tillage. Yields of brassica crops were greatly affected by tillage intensity, owing to a marked reduction of clubroot (Plasmodiophora brassicae Wor.) infection with reduced tillage. Average yields for these crops were 23%, 52% and 59% higher with deep tine cultivation, shallow tine cultivation and minimum tillage, respectively, than with plough tillage. The effects were particularly dramatic in the case of fodder rape (Brassica napus L. ssp. oleifera Sinsk. f. biennis Reichb.) and cabbage (Brassica oleracea L. var. capita L.). Liming raised the yields of some brassica crops but did not influence the effect of tillage.

Soil acidity was measured twice during the trial period and again in the second residual year, and showed values which were 0.1–0.3 pH units lower with reduced tillage than with plough tillage. This rules out the conclusion that the effect of tillage on clubroot was associated directly with acidity. Positive residual effects of reduced tillage systems were found on the yields of both brassicaceous and gramineous crops. Reduced tillage intensity may thus be recommended for all crops studied, with the exception of fodder beet, on morainic loam soils of southeast Norway.     

The contribution of agricultural practices to nitrous oxide emissions in semi-arid Mali

The yield and flux of nitrous oxide (N₂O) emitted from continuous cereals (with and without urea), legumes/cereal in rotation and cereal/legume in rotation all with or without organic manure was monitored from January 2004 to February 2005. All treatments except continuous cereals had phosphate added. The cereal grown July–October in 2003 and 2004 was pearl millet ( Pennisetum glaucum) and the legume was a bean ( Phaseolus vulgaris ). The 10 m × 10 m plots were established in a semi-arid climate in Mali. The addition of organic manure and both inorganic fertilizers increased yield and N₂O emissions. Continuous cereals treated with both organic manure and urea emitted significantly less N₂O (882 g N/ha per year) than plots receiving no organic manure(1535 g N/ha per year). Growing N-fixing crops in rotation did not significantly increase N₂O emissions. This study supports the new practice of growing cereal and legumes in rotation as an environmentally sustainable system in semi-arid Mali.     

Lead Toxicity in Cereals and Its Management Strategies: a Critical Review

Cereal grains such as wheat, rice, and maize are widely consumed as a staple food worldwide. Lead (Pb) is one of the non-essential trace elements and its toxicity in crops especially cereals is a widespread problem. The present review highlighted Pb toxicity in cereal and management strategies to reduce its uptake in plants. Lead toxicity reduced the cereal growth, photosynthesis, nutritional value, yield, and grain quality. The response of cereals to excess varies with plant species, levels of Pb in soil, and growth conditions. Reducing Pb bioavailability in the soil is a viable approach due to its non-degradability either by microbes, chemicals, or other means. Cultivation of low Pb-accumulating cultivars may reduce the risk of Pb toxicity in plants and humans via the food chain. Use of plant growth regulators, microbes, organic, and inorganic amendments might be promising techniques for further decreasing Pb contents in shoot and grains. Soil amendments along with selecting low Pb-accumulating cultivars might be a feasible approach to get cereal grains with low Pb concentrations. Furthermore, most of the studies have been conducted under controlled conditions either in hydroponic or pots and less is known about the effects of Pb management approaches under ambient field conditions.