| 猪细小病毒的PCR检测、分离及鉴定 |
| |
| 引用本文： | 刘金彪,姚月琴,高崧,彭大新,刘秀梵.猪细小病毒的PCR检测、分离及鉴定[J].中国兽药杂志,2008,42(12). |
| |
| 作者姓名： | 刘金彪 姚月琴 高崧 彭大新 刘秀梵 |
| |
| 作者单位： | 扬州大学农业部畜禽传染病学重点开放实验室,江苏扬州,225009 |
| |
| 基金项目： | 国家十一五支撑计划
，
江苏省农业三项工程项目
|
| |
| 摘 要： | 根据GenBank中报道的猪细小病毒基因组序列,利用PCR引物设计软件,设计并合成了1对引物,通过对PCR反应条件的优化,成功地扩增出了511bp的目的片段,建立了PCR检测PPV的方法。利用该方法对120份临床样品进行检测,共检测到4份阳性样品,并成功地分离到2株PPV,1#病毒分离株理化特性鉴定表明其符合PPV的特性。
|
| 关 键 词： | 猪细小病毒 分离 鉴定 |
PCR Detection, Isolation and Identification of Porcine Parvovirus |
| |
| LIU Jin-biao,YAO Yue-qin,GAO Song,PENG Da-xin,LIU Xiu-fan.PCR Detection, Isolation and Identification of Porcine Parvovirus[J].Chinese Journal of Veterinary Drug,2008,42(12). |
| |
| Authors: | LIU Jin-biao YAO Yue-qin GAO Song PENG Da-xin LIU Xiu-fan |
| |
| Abstract: | Based on the gene sequences of porcine parvovirus(PPV)in GenBank,a pair of primers was designed and synthesized.Expected fragment was successfully amplified by PCR,which was about 511 base pair.Four positive samples out of 120 clinical samples were detected with this technique.Two strains of PPV were successfully isolated by cell culture.The physico-chemical property of No.1 strain was consistent with the feature of PPV. |
| |
| Keywords: | PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
