| 露水草提取物质量标准的研究 |
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| 引用本文： | 谭梅.露水草提取物质量标准的研究[J].中国兽药杂志,2009,43(8):28-30. |
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| 作者姓名： | 谭梅 |
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| 作者单位： | 云南省兽药饲料检测所,昆明,650021 |
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| 摘 要： | 建立了露水草提取物的质量标准。用薄层色谱法对其主要成分β-蜕皮激素进行鉴别；以高效液相色谱法对露水草提取物中β-蜕皮激素进行定量分析，流动相为乙腈-甲醇-水（1：2：4），流速为1mL／min，检测波长为243nm。结果薄层色谱无干扰，专属性强；β-蜕皮激素质量浓度在0．6～4．2mg／mL范围内与峰面积线性关系良好，r=0．99997（n=7），加样回收率为99．7％，RSD为0．6％（n=6）。所用方法简便、准确、重现性好，可用于露水草提取物的质量控制。
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| 关 键 词： | 露水草提取物 β-蜕皮激素 高效液相色谱法 质量标准 |
| 收稿时间： | 2008/12/2 0:00:00 |
| 修稿时间： | 2009/4/21 0:00:00 |
Quality Standard for Extractum Cyanotis Vaga Siccus |
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| tanmei.Quality Standard for Extractum Cyanotis Vaga Siccus[J].Chinese Journal of Veterinary Drug,2009,43(8):28-30. |
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| Authors: | tanmei |
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| Institution: | Yunnan Institute of Veterinary Drug Control |
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| Abstract: | The quality standard for Extractum Cyanotis Vaga siccus was established with a TLC method for indentified beta -hydroxyecdysone in Extractum Cyanotis Vaga siccus and a HPLC method for determining simultaneously beta- hydroxyecdysone in Extractum Cyanotis Vaga siccus. The mobile phasa was made up of acetonitrile - methanol - water （ 1 ： 2 ： 4）, the flow rate was 1 mL/min, and the ultraviolet detection was set at 243 nm. The quantitative evaluation of beta - hydroxyecdysone had the linear range of 0.6 - 4.2 mg/mL （ r = 0. 999 97 ,n =7）, the average recovery was 99.7% （RSD =0. 6% ,n =6）. This method is simple and accurate with high specificity and good repeatability. It is suitable for quality control of Extractum Cyanotis Vaga siccus. |
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| Keywords: | Extractum Cyanotis Vaga siccus beta-Hydroxyecdysone HPLC quality standard |
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