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高效液相色谱法测定杨树花口服液中水杨苷的含量
引用本文:韩立.高效液相色谱法测定杨树花口服液中水杨苷的含量[J].中国兽药杂志,2014,48(6):25-27.
作者姓名:韩立
作者单位:河南省兽药监察所
摘    要:建立了高效液相色谱分离测定杨树花口服液中水杨苷含量的方法。采用Waters XBridge C18色谱柱(150mm×4.6mm,5μm),以甲醇-水(10:90)为流动相,流速为1.0mL/min,检测波长269nm,柱温30℃。水杨苷的线性范围为0.025~0.5mg/mL(r=0.9995),平均加样回收率(n=6)为100.8%,RSD为1.49%。建立的方法准确、快速,操作简便,可用于杨树花口服液中水杨苷的含量测定。

关 键 词:杨树花口服液  水杨苷  含量测定  高效液相色谱法
收稿时间:2014/3/14 0:00:00
修稿时间:2014/5/26 0:00:00

Determination of salicin in Yangshuhua Oral Liquid by HPLC
HAN Li,BAN Fu-guo,ZHOU Hong-xia,LIU Su-mei,ZHANG Yue-jing.Determination of salicin in Yangshuhua Oral Liquid by HPLC[J].Chinese Journal of Veterinary Drug,2014,48(6):25-27.
Authors:HAN Li  BAN Fu-guo  ZHOU Hong-xia  LIU Su-mei  ZHANG Yue-jing
Institution:Henan Institute of Veterinary Drugs Control,Zhengzhou
Abstract:To use the high performance liquid chromatography (HPLC) to determine salicin in Yangshuhua oral liquid. The isolation was performed on a Waters XBridge C18 column ( 150 mm×4.6 mm, 5 μm) with the mobile phase consisting of methanol-water ( 10 : 90). The flow rate was 1.0 mL/min, The detection wavelength of 269 nm and the column temperature was set at 30 ℃. HPLC was successfully applied to the isolation of salicin in Yangshuhua oral liquid. The standred curves were linear over the ranges of 0.025- 0.5 mg/mL (r = 0.9995 )for salicylic acid .The average recoveries were 100.8% ,and RSD were 1.49%.The method is simple and can be used as a quality control for Yangshuhua oral liquid.
Keywords:Yangshuhua Oral Liquid  salicin  determination  HPLC  
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