| 万乳康中盐酸左旋咪唑含量测定方法的研究 |
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| 引用本文： | 刘素梅.万乳康中盐酸左旋咪唑含量测定方法的研究[J].中国兽药杂志,2010,44(2):26-28. |
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| 作者姓名： | 刘素梅 |
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| 作者单位： | 河南省兽药监察所,郑州,450008 |
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| 摘 要： | 建立了万乳康中盐酸左旋咪唑含量测定的高效液相色谱法。样品经稀乙醇溶液提取,进高效液相色谱仪进行检测。色谱条件:Venusil XBP C18(2)(5μm,4.6 mm×150 mm)色谱柱,流动相为乙腈-0.1 mol/L磷酸溶液(用二乙胺调pH 6.8)(28∶72),检测波长214 nm,进样量为10μL。盐酸左旋咪唑的浓度在5~200μg/mL范围内与峰面积呈良好的线性关系,线性回归方程为y=44.806x+73.099,相关系数r=0.999 7。该方法简便,灵敏度高,可以更好地控制万乳康的质量。
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| 关 键 词： | 万乳康 盐酸左旋咪唑 高效液相色谱法 |
| 收稿时间： | 5/6/2009 12:00:00 AM |
| 修稿时间： | 6/9/2009 12:00:00 AM |
Determination of Levamisole Hydrochloride in Wan Rukang |
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| Liu su mei.Determination of Levamisole Hydrochloride in Wan Rukang[J].Chinese Journal of Veterinary Drug,2010,44(2):26-28. |
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| Authors: | Liu su mei |
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| Institution: | LIU Su-mei,LI Hua-cen,HAN Li,XU Yin-feng,ZANG He-ying,ZHANG Yao-jing,WANG Jun-hong(Henan Provincial Supervising Agency of Veterinary Drugs,Zhengzhou 450002,China) |
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| Abstract: | A method of HPLC for determination of levamisole hydrochloride in Wan Rukang was developed.Levamisole hydrochloride extracted from Wan RuKang was determined on HPLC.The liquid chromatography separation was carried on Venusil XBP C18（2）（5 μm,4.6 mm×150 mm）with acetonitrile-0.1mol/L H3PO4（28∶72） as mobile phase with the detection wavelength 214 nm.The equation was y=44.806x＋73.099,r=0.999 7,the calibration curve was linear in the range of 5~200 μg/mL.The method with high sensitivity was simple,and provided the scientific evidence for the research of the quality standard of Wan Rukang. |
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| Keywords: | Wan Rukang levamisole hydrochloride HPLC |
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