HPLC法测定兽用中药散剂中非法添加利巴韦林的方法研究

建立了高效液相色谱法检测白龙散、黄连解毒散、苍术香连散等7种兽用中药散剂中非法添加利巴韦林的方法。用十八烷基硅烷键合硅胶色谱柱，以水（用稀硫酸调节pH值至2．5±0.1）为流动相，检测波长207nm，流速1．0mL／min，进样量20μL。结果显示，利巴韦林的线性范围为1.016～20.32μg／mL，线性方程为Y=5．68×10X-5．61×10^-2（r=0．9999，n=6），本方法的回收率为81．28％～119．46％，RSD为0．88％～1．65％。结果表明，本方法准确、可靠，适用于兽用中药散剂中非法添加利巴韦林的检测。

The Method Study on Determination of Ribavirin Added Illegally in Veterinary Chinese Materia Medica Powders by HPLC

A method for the highly effective liquid phase chromatography detection on illegally added ribavirin in veterinary Chinese materia medica powders as Bailong San,Cangzhuxianglian San,and Huanglianjiedu San,etc was established.A reverse-phase C18 column was used,the mobile phase was water(adjusted the pH value with dilute sulphuric acid of 2.5±0.1),the determination wave length was 207 nm,flow rate was 1.0 mL/min.The results demonstrated that the linearity range of ribavirin was 1.016~20.32 μg/mL,the linear equation was Y=5.68×10X-5.61×10-2(r=0.9999,n=6),this method’s recovery rate was 81.28%~119.46%,RSD was 0.88%~1.65%.It was indicated that this method was accurate,reliable,and suitable for the determination of illegally added ribavirin in veterinary Chinese materia medica powders.