| 鸭肝炎病毒VP1蛋白B细胞抗原表位预测及变异分析 |
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| 引用本文： | 黄良宗,刘鳗仪,王淑敏,司兴奎,马春全,顾万军.鸭肝炎病毒VP1蛋白B细胞抗原表位预测及变异分析[J].中国家禽,2011,33(20). |
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| 作者姓名： | 黄良宗 刘鳗仪 王淑敏 司兴奎 马春全 顾万军 |
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| 作者单位： | 1. 佛山科学技术学院,广东佛山,528231
2. 佛山市三水区南山动物防疫站,广东佛山,528000 |
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| 基金项目： | 国家自然科学基金，国家自然科学基金 |
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| 摘 要： | 以鸭肝炎病毒(DHV)SN株VP1基因序列为基础,运用Garnier-Robson、Chou-Fasman和Karplus-Schulz方法预测VP1蛋白的二级结构,并分别运用Kyte-Doolittle方法、Jameson-Wolf方法和Emini方法预测蛋白的亲水性、抗原指数和表面可及性,最后结合吴玉章的方法综合分析预测其B细胞抗原表位.结果显示,VP1蛋白C端第132～137、177～186、209～219区段有较好的亲水性、表面可及性和较高的抗原指数,并且在二级结构上含有易形成抗原表位的无规则卷曲和β-转角,可能是VP1蛋白的B细胞抗原优势表位；DHV SN株与弱毒疫苗株在推测的VP1 B细胞抗原表位177～186和209～219两个区域氨基酸序列变异较大.
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| 关 键 词： | 鸭肝炎病毒 VP1蛋白 二级结构 B细胞抗原表位 |
B Cell Epitopes Prediction and Variations of Duck Hepatitis Virus VP1 Protein |
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| HUANG Liangzong,LIU Manyi,WANG Shumin,SI Xingkui,MA Chunquan,GU Wanjun.B Cell Epitopes Prediction and Variations of Duck Hepatitis Virus VP1 Protein[J].China Poultry,2011,33(20). |
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| Authors: | HUANG Liangzong LIU Manyi WANG Shumin SI Xingkui MA Chunquan GU Wanjun |
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| Institution: | HUANG Liangzong1,LIU Manyi2,WANG Shumin1,SI Xingkui1,MA Chunquan1,GU Wanjun1**(1.Foshan University,Foshan,Guangdong 528231,2.Epidemic Diseases Prevention and Quarantine of Nanshan,Guangdong 528000) |
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| Abstract: | The secondary structure of VP1 of duck hepatitis virus(DHV) SN strain was predicted with the methods of Garnier-Robson,Chou-Fasman and Karplus-Schulz.Its hydrophilicity,surface probability plot and antigenic index were obtained by the methods of Kyte-Doolittle,Emini and Jameson-Wolf,respectively.Then the method of WU Yuzhang was integrated to analyze the B cell epitopes.The results showed that the B cell epitopes of the VP1 protein were located at the C-terminal 132 to 137,177 to 186 and 209 to 219 regions ... |
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| Keywords: | duck hepatitis virus VP1 protein secondary structure B cell epitopes |
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