Detection of staphylococcal enterotoxin A, B, and C in milk by an ELISA procedure |
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Authors: | O Olsvik S Myhre B P Berdal K Fossum |
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Institution: | Norwegian Defence Microbiological Laboratory, National Institute of Public Health, Department of Microbiology and Immunology, Veterinary College of Norway, Oslo, Norway |
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Abstract: | Enterotoxigenic reference strains of Staphylococcus aureus were cultivated in sterile whole and skim milk for 18 h at 37°G. Staphylococcal enterotoxin A, B, and C were detected directly in the milk by an enzyme linked immunosorbent assay (ELISA), sensitive down to 1 ng/ml. Enterotoxins in the range of 1 ng–20 µg/ml milk were detected without any concentration or extraction. Skim and whole milk were almost identical as medium for enterotoxin production. |
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Keywords: | Stapholococcuc aureus enterotoxin A B and C detection milk ELISA |
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