| 胶体金试纸条法和液相色谱-串联质谱法检测牛奶中四环素类药物残留比较 |
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| 引用本文： | 汪善良,吴鹏,刘琳,赵正苗,陶光灿,何方洋.胶体金试纸条法和液相色谱-串联质谱法检测牛奶中四环素类药物残留比较[J].乳业科学与技术,2011,34(6):268-271. |
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| 作者姓名： | 汪善良 吴鹏 刘琳 赵正苗 陶光灿 何方洋 |
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| 作者单位： | 北京勤邦生物技术有限公司,北京,102206 |
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| 摘 要： | 采用胶体金试纸条与液相色谱-串联质谱（LC-MS/MS）两种方法检测牛奶中残留的四环素类药物。结果表明：胶体金试纸条的检测限为四环素40μg/L、强力霉素40μg/L、金霉素40μg/L、土霉素80μg/L;LC-MS/MS法在牛奶中四环素、强力霉素、金霉素、土霉素的检测限为50μg/L。采用两种方法对实际样品检测,检测结果一致。与液相色谱-串联质谱法相比,胶体金试纸条具有操作简便、快速、直观的特点,可作为筛选法对样品进行定性筛查,LC-MS/MS法进行阳性样品的确证和精确定量。
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| 关 键 词： | 四环素类药物 胶体金试纸条 液相色谱-串联质谱法 牛奶 |
A Comparison between Colloidal Gold Strip and Liquid Chromatography-Tandem Mass Spectrometry for Determination of Tetracyclines Residue in Milk |
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| WANG Shan-liang,WU Peng,LIU Lin,ZHAO Zheng-miao,TAO Guang-can,HE Fang-yang.A Comparison between Colloidal Gold Strip and Liquid Chromatography-Tandem Mass Spectrometry for Determination of Tetracyclines Residue in Milk[J].JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY,2011,34(6):268-271. |
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| Authors: | WANG Shan-liang WU Peng LIU Lin ZHAO Zheng-miao TAO Guang-can HE Fang-yang |
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| Institution: | (Beijing Kwinbon Biotechnology Co.Ltd.,Beijing 102206,China) |
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| Abstract: | The two different analytical methods,colloidal gold strip（CGS） and liquid chromatography-tandem mass spectrometry（LC-MS/MS）,were used to determine tetracyclines residue in milk and the analytical results were compared.CGS showed a detection limit of 40 μg/L for tetracycline,chlortetracycline and doxycycline and of 80 μg/L for oxytetracycline.The detection limits of LC-MS/MS for the tetracyclines were all 50 μg/L.Identical results were obtained for the determination of real samples by both methods.Over LC-MS/MS,CGS had the advantages of ease of use,rapidity and visualization and could allow qualitative analysis of positive samples.LC-MS/MS was suitable for the validation of positive samples and accurate quantification. |
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| Keywords: | tetracyclines colloidal gold strip liquid chromatography-tandem mass spectrometry（LC-MS/MS） milk |
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