| 布鲁氏菌pcDNA3．1-omp17．3基因疫苗的研制 |
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| 引用本文： | 邓小红,曾政,王希良.布鲁氏菌pcDNA3．1-omp17．3基因疫苗的研制[J].中国兽医科技,2007,37(6):505-509. |
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| 作者姓名： | 邓小红 曾政 王希良 |
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| 作者单位： | [1]重庆工商大学药物化学与化学生物学研究中心,重庆400067 [2]重庆市动物疫病预防控制中心,重庆401147 [3]军事医学科学院微生物流行病研究所,北京100071 |
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| 基金项目： | 国家自然科学基金项目（30170853） |
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| 摘 要： | 采用PCR方法扩增了布鲁氏菌17．3ku外膜蛋白编码基因，并将该基因克隆至真核表达载体pcDNA3．1（＋）中，成功构建了真核表达质粒pcDNA3．1-ompl7．3。pcDNA3．1-omp17．3转染coS-7细胞后，通过Western—blotting检测到了17．3ku蛋白的瞬时表达。将pcDNA3．1-ompl7．3免疫小鼠，三免后经ELISA、流式细胞仪以及ELISPOT技术检测到pcDNA3．1-omp17．3在小鼠体内诱导产生了以Th1型为主的细胞免疫应答。结果表明，构建的基因疫苗可作为潜在的布鲁氏菌新型疫苗，有进一步研究的意义。
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| 关 键 词： | 布鲁氏菌 17．3 ku蛋白 基因疫苗 免疫应答 |
| 文章编号： | 1673-4696（2007）06-0505-05 |
| 收稿时间： | 2007-01-15 |
| 修稿时间： | 2007-05-17 |
Development of DNA vaccine pcDNA3.1-omp17.3 against Brucella |
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| DENG Xiao-hong , ZENG Zheng , WANG Xi-liang.Development of DNA vaccine pcDNA3.1-omp17.3 against Brucella[J].Chinese Journal of Veterinary Science and Technology,2007,37(6):505-509. |
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| Authors: | DENG Xiao-hong ZENG Zheng WANG Xi-liang |
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| Abstract: | The ompl7.3 gene of Brucella abortus was amplified by PCR and then the amplicon was cloned into the eukaryotic expression plasmid pcDNA3.1 to construct a recombinant plasmid pcDNA3. 1- omplT. 3. Then the recombinant plasmid pcDNA3. 1-omplT. 3 was transfected into COS7 cells, and the expressed OMP 17. 3 was detected by Western-blotting. The recombinant plasmid was injected into BALB/c mice intramuscularly. The immunized mice were induced to produce a typical T helper 1 dominated immune response, as determined by ELISA, Flow Cytometer and ELISPOT method. The results Showed that pcDNA3.1-omp17. 3 was a potential candidate vaccine for the controlling of brucellosis. |
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| Keywords: | Brucella 17 3 ku protein gene vaccine immune response |
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