甬优17作烟后稻机插高产栽培技术

通过近2 a对甬优17作烟后稻机插栽培试验示范观察,总结了适时播种,培育壮秧,合理密植,科学管理肥水和防治病虫害等高产栽培配套技术,以期为"春烟—晚稻"模式种植区的粮食增产提供参考。     

Breast cancer stem cells cultured without insulin and effect of estrogen induction

AIM:To determine whether suspension culture medium without insulin can be used to feed breast cancer tumorsphere, or not. METHODS:MCF7 cells were used to build tumorsphere. The morphological changes, CD44+ CD24- expression, aldehyde dehydrogenase 1 (ALDH1) expression and multiple division ability were measured to identify the breast cancer stem cells and to detect the function of 17β-estradiol (E2β) in tumorsphere of MCF7 cells. RESULTS:The tumorshere, each containing 30 to 60 cells, was obtained by the method of insulin-removal suspension culture. These cells were cytokeratin 18 and CD10 proteins positive, and the number of CD44+ CD24- cells and ALDH1 protein expression were significantly higher than the adherent cultured cells (P<0.05). Using 10-10 mol/L E2β to treat the tumorshere for 7 d, the tumor cell number and volume were significantly increased. Using 10-10 mol/L E2β to treat the tumorshere for 24 h, the CD44+ CD24-cells and ALDH1 protein expression were significantly higher than those in non-treatment group (P<0.05). CONCLUSION: Suspension culture medium without insulin can be used to feed breast cancer tumorsphere. These tumorsphere could be used as a model to determine the function of E2β in breast cancer stem cell research.     

17O-核磁共振法研究农药微乳剂浊点与 其组份之间的相关性

农药微乳剂浊点是微乳剂的主要质量技术指标之一。研究了3种不同种类农药的微乳剂组份与浊点的关系,并利用17O-核磁共振(17O-NMR)方法对其机理进行了探究。研究表明:浊点与表面活性剂、助表面活性剂与水之间形成的氢键强度成正相关;随着水质硬度的上升,浊点下降。建议选用亲水性强的表面活性剂和极性较强的助表面活性剂来配制微乳剂,同时为了节约成本和便于工厂生产,可采用自来水来配制农药微乳剂,而采取其他措施来提高农药微乳剂的浊点。     

禽呼肠病毒P10、P17非结构蛋白基因的克隆及序列分析

根据GenBank上的禽呼肠病毒(ARV)S1基因序列,设计并合成了一对跨越P10和P17非结构蛋白基因的特异性引物,对13个ARV毒株进行RT-PCR扩增、克隆及序列测定。结果显示,13个ARV毒株的P10蛋白基因ORF全长均为297bp,编码98个氨基酸;P17蛋白基因ORF全长为441bp,编码146个氨基酸。这13个ARV毒株P10、P17蛋白基因核苷酸同源性分别在96.6%~100%和95.2%~99.3%之间,推导的氨基酸同源性分别在98.2%~100%和91.9%~99.0%之间。将这13个ARV毒株与GenBank上其他正呼肠病毒毒株,包括番鸭株(DRV)和飞狐上分离到的内尔森海湾病毒(NelsonBayvirus,NBV)及两个澳洲分离株(ARM-1和SOM-4)进行同源性比较和遗传进化树分析,结果表明,呼肠病毒有地域和种类的差别。     

禽呼肠孤病毒P17蛋白基因在大肠杆菌中的表达及其ELISA检测方法的建立

用设计合成的1对跨越禽呼肠孤病毒（ARV）P17非结构蛋白基因完整开放阅读框（ORF）的特异性引物，对ARVS1133株进行了RT-PCR扩增。扩增产物与pGEX-4T-1原核表达载体连接后，转化大肠杆菌BL21感受态细胞。经0．2mmol／LIPTG诱导，表达的融合蛋白分子质量为42.4ku，占茵体总蛋白的34％。表达产物P17蛋白经不同浓度尿素纯化后，纯度达到85％。Western-blotting显示，纯化的P17蛋白能与感染ARV的阳性血清反应，说明其具有抗原性。以此重组蛋白为包被抗原初步建立了用于鉴别检测ARV活病毒感染与灭活疫苗免疫的SPF鸡血清的ELISA。     

抑菌霉素A17对12种病原真菌及3种害虫的生物活性

从稀有放线菌7310菌株的发酵菌丝中经溶媒萃取和硅胶柱层析初步分离出来一种活性成分，经结构鉴定为新结构化合物——抑茵霉素A17。抑茵霉素A17杀虫活性结果表明，对家蝇具有很强的杀虫活性，其LC50值仅为1．56μg／ml，并能在较短的时间内击倒供试虫体；对菜粉蝶和小菜蛾幼虫也具有较强的杀虫活性，LC50值分别为106．27和172．9μg／ml。以常见的12种病原真菌作为供试菌，在室内进行了抑菌霉素A17抑菌活性的定性及定量分析，结果表明抑菌霉素A17硅胶层析粗提物对12种供试真菌中的甘薯软腐病病菌、柑桔酸腐病病菌、桃黑星病病菌、番茄早疫病病菌、番茄芽枝病病菌、莴苣菌核病病菌、荔枝霜霉病病菌、炭疽菌、弯孢霉、拟茎点霉和果腐葡萄孢的菌丝生长有明显的抑制作用，其中对拟茎点霉的活性最强，抑制中浓度仅为0．1μg／ml。     

长期干旱胁迫下Mdm-miR160- MdARF17- MdHYL1模块调控苹果生长发育的研究

为了研究长期干旱条件下Mdm-miR160-MdARF17-MdHYL1 调控网络在苹果生长发育中的作用，以GL-3和Mdm-miR160e OE、MdARF17 RNAi、MdmARF17 OE、 MdHYL1 OE及MdHYL 1 RNAi转基因苹果为试验材料，进行为期3个月的长期干旱处理，测定苹果株高、根干质量、根冠比以及叶片净光合速率、胞间CO₂浓度、气孔导度、蒸腾速率、叶绿素荧光值和水分利用效率。结果表明，在长期干旱胁迫下， Mdm-miR160e OE转基因苹果植株更矮化，根冠比、根干质量、光合能力及水分利用效率大于GL-3植株，具有更强的耐旱性； MdARF17 RNAi植株比GL-3更矮，且具有更大的根冠比、根干质量、光合能力和水分利用效率，而MdARF17 OE与之相反； MdHYL1 RNAi植株对长期干旱胁迫更敏感，其株高大于野生型且具有更小的根冠比、更低的光合能力和水分利用效率，而 MdHYL1 OE植物对长期干旱条件具有更强的适应性，生长发育表型与RNAi植株相反。综上所述，Mdm-miR160- MdARF17-MdHYL1调控网络在苹果响应长期干旱过程中发挥了重要功能，为苹果抗旱分子育种提供了候选基因。     

优质甘蓝型油菜黔油17号亲本原种的保优繁殖技术研究

通过优质甘蓝型油菜隐性核不育系98-116 AB与父本ZW 99004单株成对杂交,并在F1代杂交组合进行配合力测定,获得高配合力的成对株系材料。根据优质杂交油菜新品种黔油17号母本98-116 AB及父本ZW 004的自身特点,分别在人工隔离和自然隔离条件下进行其原原种及原种的保优繁殖技术研究,保证亲本原始的优良种性,确保大面积生产中杂交种的质量以及品种的生产潜力。     

Hormonal changes during meiotic maturation and ovulation in the brook trout (Salvelinus fontinalis)

The plasma levels of estradiol-17 (E2), 17, 20-dihydroxy-4-pregnen-3-one (17,20-P) and gonadotropin (GTH) were measured in brook trout (Salvelinus fontinalis) during the period from the end of vitellogenesis to postovulation. Blood samples were taken according to specific stages of maturation, including germinal vesicle breakdown (GVBD) and ovulation. E2 levels were quite high (45 ng/ml) at the end of vitellogenesis (and prior to GVBD) and dropped precipitously by GVBD (2 ng/ml). They remained low through ovulation and postovulation. 17,20-P levels were low prior to GVBD (0.7 ng/ml) and increased dramatically at GVBD (148 ng/ml). The levels of 17,20-P remained high at ovulation (142 ng/ml) and then dropped significantly within 24 h to approximately half of the ovulatory values. They decreased even further by 7 days postovulation. GTH levels rose gradually through GVBD and ovulation from a postvitellogenic level of approximately 3 ng/ml to a 7 day postovulatory value of approximately 10 ng/ml. The overall results; 1) decrease in estradiol prior to GVBD, 2) increase in 17,20-P at GVBD and 3) gradual GTH rise through GVBD and ovulation, are similar to those reported for other salmonids.