禽网状内皮组织增生病病毒和马立克氏病病毒共感染对鸡的致肿瘤作用

用禽网状内皮组织增生病病毒(REV)和马立克氏病病毒(MDV)共感染肉鸡，研究这2种病毒对鸡的致瘤作用，结果表明肉鸡共感染MDV和REV后13d即可发生死亡．接种后100d死亡率达84％。死亡鸡的肝脏、脾脏、肾脏和心脏等可以形成2种外观明显不同的散在的大肿瘤结节和弥漫性的小肿瘤结节。取发病鸡的肝脏、脾脏、肾脏、心脏和腺胃等组织样品做连续石蜡切片，HE染色后发现这些脏器均存在2种不同类型的肿瘤细胞增生。对这些连续切片分别用MDV和REV的单克隆抗体进行间接荧光试验，则同1份样品存在可以与REV和MDV分别呈现阳性反应的肿瘤细胞团，结果表明REV和MDV可以在感染鸡的体内分别诱发形成肿瘤。在接种后14、21、28和42d随机采集3只鸡的全血分离MDV和REV，均可以同时分离到MDV和REV。表明REV和MDV的共感染延长了病毒血症的时间。

Pathogenesis of Co-infection on Broiler Chicken by Marek's Disease Virus and Reticuloendotheliosis Virus

Pathogenesis were studied by inoculating the broiler chickens with reticuloendotheliosis virus(REV) and Marek's disease virus (MDV) . After 13 days post-inoculation, broiler chickens began to die and the mortality reached 84% at the end of experiment. By gross examination, there were two different tumors of sporadic, large tumors and diffusible, small tumors that induced and mixed on the aspect of liver, spleen, kidney and heart. These samples were embedded with paraffin and cut into a series of segments that were stained with hematoxylin and eosin, and examined for existence of two different kinds of tumor cells by microscopic lesions. Some segments were reacted with monoclonal antibody of MDV BA4 and REV 11B118 respectively. The IFA examinations showed that both MDV and REV antibody could react with the same segment, which meant REV and MDV could induce tumors separately in chickens.14,21, 28 and 42 days after inoculated, both MDV and REV were isolated from three blood samples that were collected at random. The results showed that co-infection of MDV and REV could prolong the date of virus existence in the blood.