| 猪链球菌2型溶血素基因缺失株构建及其生物学特性分析 |
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| 引用本文： | 吴炜,张晓燕,唐宇龙,方丽华,方维焕.猪链球菌2型溶血素基因缺失株构建及其生物学特性分析[J].动物医学进展,2012,33(5):22-26. |
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| 作者姓名： | 吴炜 张晓燕 唐宇龙 方丽华 方维焕 |
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| 作者单位： | 浙江大学动物预防医学研究所,浙江省动物预防医学重点实验室,浙江杭州310058 |
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| 摘 要： | 猪链球菌溶血素(SLY)是较早确定的猪链球菌毒力因子之一,具有细胞毒性,在猪链球菌致病过程中发挥重要作用。利用同源重组技术成功构建了猪链球菌2型(SS2)sly基因缺失的突变株SS2-Δsly,缺失株的体外溶血活性消失,对小鼠脑血管内皮细胞的细胞毒性显著低于亲本菌株(P<0.01),但不影响猪链球菌2型在巨噬细胞中的存活。该菌株可以用于研究SS2溶血素在感染细胞中的作用机制。
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| 关 键 词： | 猪链球菌2型 溶血素基因缺失株 溶血性 细胞毒性 |
Construction and Characterization of Streptococcus suis type 2 Mutant Strain with Suilysin Gene Deletion |
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| WU Wei
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ZHANG Xiao-yan
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TANG Yu-long
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FANG Li-hua
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FANG Wei-huan.Construction and Characterization of Streptococcus suis type 2 Mutant Strain with Suilysin Gene Deletion[J].Progress In Veterinary Medicine,2012,33(5):22-26. |
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| Authors: | WU Wei ZHANG Xiao-yan TANG Yu-long FANG Li-hua FANG Wei-huan |
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| Institution: | (Zhejiang University Institute of Preventive Veterinary Medicine,Zhejiang Provincial Key Lab for Preventive Veterinary Medicine,Hangzhou,Zhejinag,310058,China) |
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| Abstract: | Suilysin(SLY),one of the most important virulence factors of Streptococcus suis type 2(SS2),plays an important role in the pathogenesis by its cytotoxic activity.We constructed a mutant SS2-Δsly with deletion of the sly gene from a clinical SS2 strain 1 105 by homologous recombination.Deletion of the sly gene abolished the hemolytic activity of SS2 and reduced cytotoxicity to Bend3 cell brain epithelial cells by more than 60% as compared with its parent strain.There was no significant difference between the mutant and parent strains in survival within macrophage cell lines Raw 246.7.The SS2-Δsly mutant strain could be used to explore the mechanisms of SLY in infected cells. |
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| Keywords: | Streptococcus suis type 2 sly-deletion mutant hemolytic activity cytotoxicity |
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