林可霉素-大观霉素复方混悬注射液中有效成分含量的HPLC法测定

建立了测定林可霉素-大观霉素复方油混悬注射液中盐酸林可霉素与盐酸大观霉素含量的高效液相色谱(HPLC)方法.采用C18色谱柱(4.6mm×250 mm,5 μm),检测林可霉素的流动相为0.05mol/L硼砂溶液(用850 mL/L磷酸调节pH至5.0)-甲醇-乙腈(60∶36∶4),检测波长为214 nm；检测大现霉...

Assay of Lincomycin and Spectinomycin in Oily Injectable Suspensions by HPLC

A high-performance liquid chromatographic(HPLC) method with ultraviolet-visible detection was developed to measure the concentrations of lincomycin and spectinomycin in their oily injectable suspensions.The separation was performed on a C18 column(4.6 mm×250 mm,5 μm),the mobile phase of lincomycin comprised of 0.05 mol/L borax solution(pH5.0,adjusted with 85% phosphoric acid)-methanol-acetonitril(60∶36∶4),UV detection was set at a wavelength of 214nm.the mobile phase of spectinomycin comprised of acetonitril,tetrahydrofuran and water(30∶35∶35),UV detection was set at a wavelength of 415 nm.The standard curves of lincomycin and spectinomycin were linear in the range between 10 μg/mL to 40 μg/mL,rthe average recoveries were 98.68% for lincomycin and 99.10% for spectinomycin,the average intraassay coefficients of variation were 0.93% for lincomycin and 0.86% for spectinomycin,and the interassay coefficients of variability were 1.68% and 0.98%,respectively.The present method was precise and convenient,and could therefore be used to quantify lincomycin and spectinomycin in oily injectable suspensions.