| 禽流感病毒H5N2亚型西藏株的分离鉴定与特性分析 |
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| 引用本文： | 刘志刚,赵苏红,索朗斯珠,吴彦,金梅林.禽流感病毒H5N2亚型西藏株的分离鉴定与特性分析[J].兽医大学学报,2014(1):17-20. |
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| 作者姓名： | 刘志刚 赵苏红 索朗斯珠 吴彦 金梅林 |
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| 作者单位： | [1]安庆师范学院生命科学院,安徽安庆246011 [2]华中农业大学农业微生物国家重点实验室,湖北武汉430070 |
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| 基金项目： | 国家“973”计划资助项目（2011CBS05000）,（2010cB534000）;国家科技重大专项课题（2008ZX10403,2009ZX10004-109） |
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| 摘 要： | 从西藏地区病死鸡体内分离鉴定到1株H5N2亚型禽流感病毒，基因组序列测定与分析显示，此H5N2毒株可能是由H5NI和H9N22种亚型的病毒重配形成，其PB2、HA、NS片段与高致病性H5NI亚型高度同源，PBI、PA、NP、NA、M均与低致病性H9N2亚型亲缘关系相近。尽管HA蛋白切割位点有多个碱性氨基酸序列，但静脉接种指数只有0．59，表现为低致病力。
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| 关 键 词： | 西藏 禽流感病毒 H5N2亚型 基因重配 分离鉴定 |
Isolation and identification of a H5N2 subtype avian influenza virus stain derived from Tibet |
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| Authors: | LIU Zhi-gang ZHAO Su-hong SUOLANG Si-zhu WU Yan JIN Mei-lin |
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| Institution: | 1. College of Life Sciences,Anqing Normal University, Anqing , Anhui 246011, China ; 2. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China) |
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| Abstract: | One H5N2 subtype strain of avian influenza virus （AIV） was isolated from sick chickens in Tibet. Genomic sequencing indicated that this isolate was a novel reassortant between HPAIV H5N1 and LPAIV H9N2 . The PB2,PB1 and PA are highly homology to HSN1 HPAIV while other genes have relative with H9N2. It belongs to LPAIV based on the IVPI, though multiple basic amino acids were found in the surface glycoprotein HA. |
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| Keywords: | Tibet AIV HSN2 subtype reassortant identification |
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