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| 兔病毒性出血症病毒中国株(JX/CHA/97)全基因组分子克隆及序列分析 | |
| 引用本文： | 倪征,方维焕,张玉颖,云涛,陈锦清,刘光清.兔病毒性出血症病毒中国株(JX/CHA/97)全基因组分子克隆及序列分析[J].中国预防兽医学报,2006,28(6):668-671,675. |
| 作者姓名： | 倪征 方维焕 张玉颖 云涛 陈锦清 刘光清 |
| 作者单位： | 1. 浙江大学,动物科学学院,浙江,杭州,310029;浙江省农业科学院病毒学与生物技术研究所植物代谢基因工程试验基地,浙江,杭州,310021 2. 浙江大学,动物科学学院,浙江,杭州,310029 3. 浙江省农业科学院病毒学与生物技术研究所植物代谢基因工程试验基地,浙江,杭州,310021 |
| 摘 要： | 本研究首次完成了免病毒性出血症病毒（Rabbit hemorrhagic disease virus，RHDV）中国分离株JX／CHA／97全基因组序列的测定与分析。研究结果表明，JX／CHA／97株的全基因组由7437个核苷酸组成，其基因组构成为：5＇端有一个长度仅为9nt的非编码区，随后有2个阅读框架（ORFs）．分别编码一个聚合蛋白和一个结构蛋白。在基因组的3＇端也有一个非编码区，长度为59nt。另外，采用RACE方法获得了RHDV的poly（A）尾序，证明该结构至少含有27个A。根据RHDV衣壳蛋白的基因序列，将JX／CHA／97株与来自世界各地的22株RHDV分离株的基因组序列进行了比较与分析，并绘制了系统发生树。结果表明，RHDV各分离株之间存在较高的序列同源性，可以将它们至少分为6个拓朴群，提示RHDV有朝不同方向发生进化的趋势。 |
| 关 键 词： | 兔病毒性出血症病毒 JX/CHA/97株 克隆 序列分析 |
| 文章编号： | 1008-0589（2006）06-0668-04 |
| 收稿时间： | 2005-10-20 |
| 修稿时间： | 2005-10-20 |
Molecular cloning and sequence analysis of full-length genome of RHDV JX/CHA/97 strain |
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| NI Zheng,FANG Wei-huan,ZHANG Yu-ying,YUN Tao,CHEN Jin-qing,LIU Guang-qing.Molecular cloning and sequence analysis of full-length genome of RHDV JX/CHA/97 strain[J].Chinese Journal of Preventive Veterinary Medicine,2006,28(6):668-671,675. | |
| Authors: | NI Zheng FANG Wei-huan ZHANG Yu-ying YUN Tao CHEN Jin-qing LIU Guang-qing |
| Institution: | 1. College of Animal Science and Technology, Zhejiang University, Hangzhou 310029, China; 2. Key Laboratory of Virology and Biotechnology, Zhejiang Laboratory of Plant Metabolic and Genetic Engineering, Hangzhou 310021, China |
| Abstract: | The complete nucleotide sequence of the China strain of rabbit hemorrhagic disease virus(RHDV)was determined to be 7 437 nucleotides in length with a Y-terminal non-coding region of 9 nucleotides and a 3'-terminal non-coding region of 59 nucleotides.Two open reading frames(ORFs)were found within this sequence coding for a polymerize protein and a structural protein.Besides,our data showed that the poly(A)tail of RHDV had 27 A at least.Sequence comparison studies revealed significant homology between nonstructural proteins or structural proteins of JX/CHA/97 strain and the other strains. However,the results also showed that groups of RHDV strains appeared to have evolved to different extents. |
| Keywords: | rabbit haemorrhagic disease virus JX/CHA/97 strain clone sequence analysis |
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