| H5N1亚型禽流感病毒血凝素蛋白单克隆抗体的制备及鉴定 |
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| 引用本文： | 崔佳莹,李印,于扬,张文亮,邓国华,陈化兰.H5N1亚型禽流感病毒血凝素蛋白单克隆抗体的制备及鉴定[J].中国预防兽医学报,2011,33(6). |
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| 作者姓名： | 崔佳莹 李印 于扬 张文亮 邓国华 陈化兰 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所农业部动物流感重点开放实验室/兽医生物技术国家重点实验室,黑龙江哈尔滨,150001 |
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| 基金项目： | 中国农业科学院院所长基金 |
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| 摘 要： | 为制备抗H5N1禽流感病毒(AIV)的单克隆抗体(MAb),本研究以灭活的H5N1亚型A/Chickeen/Guangdong/S2261/2009(H5N1GD261)株免疫4周龄～6周龄BALB/c雌鼠,取其脾细胞与骨髓瘤细胞SP2/0进行融合,经间接ELISA及HI筛选获得了3株能稳定分泌抗HA的MAb杂交瘤细胞,分别命名为1A4、2F5和3D3.MAb亚类鉴定表明,2F5为IgM类,其它为IgG2a亚类,轻链均为k链.经血凝抑制检测,3株MAb均能与H5亚型的AIV结合,并具有较好的型广谱性.
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| 关 键 词： | 禽流感病毒 血凝素 单克隆抗体 血凝抑制 |
Preparation and identification of monoclonal antibodies against HA protein of AIV H5N1 subtype |
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| CUI Jia-ying,LI Yin,YU Yang,ZHANG Wen-liang,DENG Guo-hua,CHEN Hua-lan.Preparation and identification of monoclonal antibodies against HA protein of AIV H5N1 subtype[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(6). |
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| Authors: | CUI Jia-ying LI Yin YU Yang ZHANG Wen-liang DENG Guo-hua CHEN Hua-lan |
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| Institution: | CUI Jia-ying,LI Yin,YU Yang,ZHANG Wen-liang,DENG Guo-hua*,CHEN Hua-lan(State Key Laboratory of Veterinary Biotechnology,Animal Influenza Laboratory of the Ministry of Agricultural,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
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| Abstract: | |
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| Keywords: | avian influenza virus HA monoclonal antibodies HI |
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