| 马立克氏病病毒感染鸡羽髓上皮细胞差异蛋白质组学研究 |
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| 引用本文： | 丛锋,刘长军,张艳萍,李志杰,张锋,成云,杨文闯,许娜娜.马立克氏病病毒感染鸡羽髓上皮细胞差异蛋白质组学研究[J].中国预防兽医学报,2011,33(6). |
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| 作者姓名： | 丛锋 刘长军 张艳萍 李志杰 张锋 成云 杨文闯 许娜娜 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/禽传染病研究室,黑龙江哈尔滨,150001 |
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| 基金项目： | 国家重点实验室基本科研业务费，中央级公益性科研院所基本科研业务费专项 |
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| 摘 要： | 为鉴定鸡羽髓上皮细胞感染马立克氏病病毒(MDV)前后差异表达的蛋白,本研究以MDV强毒GA株人工感染SPF鸡,并通过双向电泳技术进行分析.结果显示:在病毒感染后4 d、7 d、14 d和21 d显著差异表达的蛋白点分别有2个、8个、25个和9个;而通过质谱技术鉴定出29种蛋白质,其中包括能量代谢相关蛋白、增殖和凋亡相关蛋白、细胞骨架蛋白、信号传导蛋白、转录相关蛋白、免疫相关蛋白和其他功能蛋白质.本实验首次对鸡羽髓上皮细胞感染MDV后各时期蛋白表达水平的变化进行研究,鉴定了多种差异表达蛋白质,为进一步揭示MDV与宿主的相互关系、感染性病毒粒子的成熟和致病机制提供了依据.
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| 关 键 词： | 马立克氏病病毒 羽髓上皮细胞 双向电泳 蛋白质组学 |
Proteome analysis of the feather follicle epithelium from chickens infected with Marek's disease virus |
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| CONG Feng,LIU Chang-jun,ZHANG Yan-ping,LI Zhi-jie,ZHANG Feng,CHENG Yun,YANG Wen-chuang,XU Na-na.Proteome analysis of the feather follicle epithelium from chickens infected with Marek's disease virus[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(6). |
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| Authors: | CONG Feng LIU Chang-jun ZHANG Yan-ping LI Zhi-jie ZHANG Feng CHENG Yun YANG Wen-chuang XU Na-na |
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| Institution: | CONG Feng,LIU Chang-jun*,ZHANG Yan-ping,LI Zhi-jie,ZHANG Feng,CHENG Yun,YANG Wen-chuang,XU Na-na(Division of Avian Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
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| Abstract: | The feather follicle epithelium(FFE) is associated with the production of enveloped infectious virus of Marek's disease virus(MDV),which is shed along with feathers and dander in infected chickens.For this reason,the FFE are important in transmission of MDV and epidemiology of MD.In the present study,changes in the FFE proteome at 4,7,14 and 21 days post-infection in response to MDV infection were studied using two-dimensional gel electrophoresis and subsequently the protein spots were identified by MS/MS.I... |
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| Keywords: | Marek's disease virus feather follicle epithelium two-dimensional gel electrophoresis proteomics |
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