液相色谱-串联质谱法同时检测鸡肉中61种抗菌药物

为建立固相萃取结合液相色谱-串联质谱同时测定鸡肉中磺胺类、喹诺酮类、大环内酯类药物残留的分析方法,通过准确称取2 g鸡肉样品,经PBS缓冲液提取,以Agilent Pleax PCX固相萃取柱净化,最后以液相色谱-串联质谱进行检测.结果显示:在最优试验条件下测得磺胺类、喹诺酮类、大环内酯类抗菌药物线性良好,相关系数(R...

Simultaneous Determination of 61 Antibiotics in Chicken by LC-MS

In order to establish a method for simultaneous determination of sulfonamides,quinolones and macrolides residues in chicken by solid phase extraction coupled with liquid chromatograph-mass spectrometer(LC-MS),2 g chicken samples were accurately weighed,extracted by PBS,purified by an Agilent Pleax PCX solid phase extraction column,and then detected by LC-MS.The results showed that the linearity of sulfonamides,quinolones and macrolides was good unnder optimal experimental conditions,with all correlation coefficients(R²)>0.99.The quantitative limit of the method was 1.0 to 10.0μg/kg.The average recovery ranged within 62.9%to 118.2%at three concentration addition levels,and the intra-batch variable coefficients ranged within 4.3%to 18.8%.In conclusion,the established method,with good precision and accuracy,was simple and rapid for simultaneous determination of sulfonamides,quinolones and macrolides residues in chicken.