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| 猪链球菌2型四川分离株毒力基因的检测及其序列分析 | |
| 引用本文： | 赵永刚,王君玮,王志亮,赵云玲,王清华,张维,陈义平,刘华雷,王华,王伟伟,田晓灵.猪链球菌2型四川分离株毒力基因的检测及其序列分析[J].中国动物检疫,2009,26(3):25-28. |
| 作者姓名： | 赵永刚 王君玮 王志亮 赵云玲 王清华 张维 陈义平 刘华雷 王华 王伟伟 田晓灵 |
| 作者单位： | 1. 中国动物卫生与流行病学中心国家外来动物疫病诊断中心,山东青岛,266114 2. 扬州大学农学院,江苏扬州,225600 3. 四川农业大学,四川成都,610000 |
| 摘 要： | 根据猪链球菌2型的MRP、EPF和SLY基因设计合成了四对引物,对我们分离鉴定的2005四川分离株进行毒力因子检测,并对阳性产物测序比较。结果以分离株为模板,获得了与预期结果一致的867bp(MRP)、572bp(EF)的2个片段,以及SLY的长度为1502bp目的片段(外引物)和1443bp的目的片段(内引物)。说明分离株SSsc0501为MRP+EF+SLY+,属于具有3种主要毒力因子的强毒株。经对PCR阳性产物测序及序列分析,结果其与P1/7株的核苷酸序列完全一致,与1933株的同源性达99.7%,仅在128位、217位、744位、1380位、1386位的5个核苷酸发生突变。在氨基酸水平上SSsc0501株与1933株的同源性达99.6%,有2个氨基酸发生替代。 |
| 关 键 词： | 猪链球菌2型 毒力基因 检测 序列分析 |
Sequence Analysis mad Virulence-relate Genes Detection of Streptococcus Suis 2 Isolated in Sichuan Province |
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| Zhao Yonggang,Wang Junwei,Wang Zhiliang,Zhao Yunling,Wang Qinghu,Zhang Wei,Chen Yiping,Liu Hualei,Wang Hu,Wang Weiwei,Tian Xiaoling.Sequence Analysis mad Virulence-relate Genes Detection of Streptococcus Suis 2 Isolated in Sichuan Province[J].China Journal Of Animal Quarantine,2009,26(3):25-28. | |
| Authors: | Zhao Yonggang Wang Junwei Wang Zhiliang Zhao Yunling Wang Qinghu Zhang Wei Chen Yiping Liu Hualei Wang Hu Wang Weiwei Tian Xiaoling |
| Institution: | 1 (1.National Diagnostic Centre for Exotic Animal Diseases,CAHEC,MOA, Qingdao Shandong 266114;2.Yang Zhou University, Yangzhou Jiangsu 225600;3.Sichuan Agricultural University,Chengdu Sichuan 610000) |
| Abstract: | Virulence-related factors of SSsc0501, a strain of Streptococcus Suis serotype 2, isolated during the 2005 outbreak in Sichuan Province were detected and sequenced. Digested with EcoR Ⅰ, the PCR product can produce a 867bp (MRP) and a 572bp (EF) DNA fragment as expected, as well as a 1502bp (exoprimer) and a 1443bp (innerprimer) DNA fragments of SLY gene. The result revealed that SSsc0501 was a high virulent strain with MRP+EF+SLY+.The PCR product from SSsc0501 strain was sequenced and nucleotide sequence analysis revealed that the sly gene from strain SSsc0501 had a high homology to strains 1933(99.7%) and P1/7 (100%). Only 5 nucleotides developed mutants in site 128,217,744,1380 and 1386 of 1933 strain. Aminoacid analysis also demonstrated highly homologous(99.6%) with two aminoacids replaced. |
| Keywords: | Streptococcus suis 2 virulence gene detection sequence analysis |
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