原位杂交技术用于虹彩病毒对美国青蛙侵染的诊断

蛙虹彩病毒（Rana grylio virus,RGV)能引起鱼类、两栖类和爬行类水生动物严重的系统性疾病，RGV是从我国患病蛙中分离到一种虹彩病毒。体外扩增的RGV经人工方法感染幼龄美国青蛙（Rana grylio)，运用原位杂交技术，分别对感染1-3d后的蛙心、肺、肾、肠、脾、肝等6种组织进行RGV分子定位和检测，结果显示，在幼蛙的肺和肠中有较强的阳性信号，在其他组织中也检测到RGV的存在。本试验探讨了RGV感染早期在宿主体内的增殖及在不同组织中的分布状况，建立了一种彩虹病毒的早期诊断方法，并为揭示RGV的致病机制奠定了基础。

In situ Hybridization Detection of RGV in Tissues of the Experimentally Infected Pig Frog

Ranavirus is one kind of pathogen belong to the group of iridoviruses,it has been recognized as a cause of serious systemic dis eases among fishes,amphibious and reptiles in the recent years.A pathogenic viru s,provisionally designated RGV(Rana grylio virus),was isolated from the dise ased pig frog in China,RGV was amplified in vitro and the juvenile pig frogs were infected.In situ hybridization was used for detecting RGV in heart,lun g,kidney,intestine,spleen and liver from the artificially infected juvenile pig frogs.The results showed RGV was detected in these tissues of juvenile frogs at 1 d,2 d,3 d post-infection.No positive signs were observed in these tissues fro m negative control.Stronger positive signs were appeared in lung and intestine t issue specimens,and the positive signs distributed diffusely in the other four t issues.