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猪瘟合并猪繁殖与呼吸综合征病毒(PRRSV)和猪圆环病毒2型(PCV2)感染
引用本文:杨宗照,方维焕.猪瘟合并猪繁殖与呼吸综合征病毒(PRRSV)和猪圆环病毒2型(PCV2)感染[J].中国兽医学报,2006,26(3):240-242.
作者姓名:杨宗照  方维焕
作者单位:1. 浙江大学,预防兽医研究所,浙江,杭州,310029;杭州市畜牧兽医总站,浙江,杭州,310020
2. 浙江大学,预防兽医研究所,浙江,杭州,310029
摘    要:2004年初以来,杭州地区的几个规模化猪场陆续发生仔猪腹泻、保育猪呼吸困难的疾病,剖检见肺部呈橡皮样,全身淋巴结水肿,部分猪脾脏边缘梗死等,发病率、死亡率较高。设计针对猪繁殖与呼吸综合征病毒(PRRSV)、猪圆环病毒2型(PCV2)的特异性引物,进行RT—PCR或PCR检测;运用伪狂犬病(PR)鉴别试剂盒检测PR,运用免疫荧光法检测猪瘟(HC),并进行了细菌分离培养,通过全面检查认为,这几个猪场发生了猪瘟合并猪PRRSV、PCV2感染。

关 键 词:猪瘟  猪繁殖与呼吸综合征病毒  猪圆环病毒2型  混合感染
文章编号:1005-4545(2006)03-0240-03
收稿时间:2004-04-07
修稿时间:2004年4月7日

HCV Co-infecting with PRRSV and PCV2
YANG Zong-zhao,FANG Wei-huan.HCV Co-infecting with PRRSV and PCV2[J].Chinese Journal of Veterinary Science,2006,26(3):240-242.
Authors:YANG Zong-zhao  FANG Wei-huan
Institution:1. Instituition of Preventive and Veterinary Medicine, Zhejiang University, Hangzhou 310029, China; 2. Hangzhou Animal Husbandary and Veterinary Station, Hangzhou 310020, China
Abstract:Nine piglets from three pig farms had diarrhea, spleen infarct, rubbery lesion in lungs and edema in allover lymphonodi. Two pairs of specific premiers were designed and synthesized, then PCR and RT-PCR were used to detect PRRSV and PCV2. They also was examined for Pseudorabies virus, HCV and bacteria. The results showed that HCV, PRRSV and PCV2 were detected in the ill piglets. HCV, PRRSV and PCV2 should be considered as the pathogens of the combined syndrome.
Keywords:HC  PRRSV  PCV2  combined infection
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