不同休眠玉米种子内源激素的含量测定和分析

本研究利用高效液相色谱法,对7个不同休眠性的玉米自交系鲜种子内源激素ZT、GA3、IAA和ABA的含量进行了测定和分析。分析了各种激素及其相互作用对玉米种子休眠的影响,为阐明玉米种子休眠机理奠定了基础。结果表明,ZT含量在一定程度上与种子休眠性呈负相关性,具有对种子萌发抑制物的拮抗作用;GA3具有解除休眠和促进萌发的功能,在种子萌发过程中是必需的;IAA与休眠不具有相关性;ABA具有拮抗GA3的作用,诱导种子休眠,抑制萌发。种子的休眠是几种激素综合作用的结果,种子休眠程度与种子内起促进作用和起抑制作用的激素之间的比例密切相关。强休眠特性玉米自交系具有以下特征:ZT和GA3含量低,ABA含量高,ZT/ABA值与GA3/ABA值较小,各激素相对平衡。

Comparative analysis of endogenous hormones in maize seeds with different dormancy characteristics

A study has been undertaken to clarify maize seed dormancy mechanisms.Four endogenous hor-mones-zeatin (ZT),gibberellins (GA3 ),indole acetic acid (IAA)and abscisic acid (ABA)in 7 maize inbred lines with diverse dormancy characteristics were measured and analysed by HPLC.We analysed the effect of the four endogenous hormones on seed dormancy,including their interaction.Results indicated that ZT content was negatively correlated with seed dormancy,with an antagonism to germination inhibitors.GA3 is necessary for germination and can help break dormancy.IAA has no correlation with seed dormancy.ABA promoted dormancy and inhibited germination,with an antagonism to GA3 .Furthermore,seed dormancy was closely re-lated to the proportion of the four hormones.Maize inbred lines with strong dormancy had the following traits:low content of ZT and GA3 ,high content of ABA,low values of ZT/ABA and GA3/ABA,and balanced hor-mone content.