小鼠急性肺损伤造模条件的探究

为探究构建急性肺损伤模型的条件，本试验选用健康昆明小鼠，经腹腔注射递增剂量脂多糖（LPS）（0、2、4、6、8、10 mg/kg），观察不同条件下小鼠呼吸频率、死亡率、肺脏干湿重比值及组织结构变化，检测炎症因子IL-10、TNF-α、IL-1β及IFN-γ在不同程度肺损伤中的表达量变化。结果显示，4 mg/kg LPS组肺脏干湿重比值显著高于对照组（P<0.05）；6、8和10 m/kg LPS组肺脏干湿重比值极显著高于对照组（P<0.01）。病理切片分析显示，6 mg/kg LPS组小鼠肺脏出现充血，肺泡腔及肺间质出现渗出，肺泡隔增厚，出现少量中性粒细胞浸润；8、10 mg/kg LPS组小鼠肺脏充血明显，肺泡腔及肺间质出现渗出，肺泡明显隔增厚，出现中性粒细胞浸润；与对照组相比，6、8、10 mg/kg LPS组肺组织中胶原纤维大量增多，且多出现在肺气管周围，10 mg/kg LPS组现象最明显。炎症因子检测分析结果显示，与对照组相比，2、4、6、8、10 mg/kg LPS组炎症因子均极显著增加（P<0.01）。结合病理组织切片及相关检测指标表明，昆明小鼠腹腔注射8 mg/kg LPS并作用12 h，可成功构建急性肺损伤模型。

Study on the Building Conditions of Acute Lung Injury in Mice

In order to explore the building conditions of acute lung injury, healthy Kunming mice were selected, by intraperitoneal injection of cascade dose of LPS (0, 2, 4, 6, 8, 10 mg/kg) to observe the mice respiratory frequency, mortality, lung W/D value and the changes of tissue structure in different conditions, and detect the expression changes of inflammation cytokine, IL-10,TNF-α, IL-1β and IFN-γ. The results showed that compared with control group, lung W/D value in 4 mg/kg LPS group significantly increased (P<0.05) and lung W/D values in 6, 8 and 10 mg/kg LPS groups extremely significantly increased (P<0.01). Pathological analysis showed that 6 mg/kg LPS group appeared lung congestion, alveolar luminal and pulmonary interstitial exudation, alveolar septal thickening, and a small amount of neutrophil infiltration. 8 and 10 mg/kg LPS groups appeared pulmonary congestion, alveolar and pulmonary interstitial exudation, alveolar septal thickening and neutrophil infiltration. Compared with control group, collagen fibers of the lung tissue in 6, 8, 10 mg/kg LPS group increased, and more appeared in the lung around the trachea, 10 mg/kg LPS group was the most obvious. The inflammatory factors in 2, 4, 6, 8 and 10 mg/kg LPS groups had extremely significantly increased compared with control group (P<0.01). Pathological biopsy and related indicators showed that the model of mice could be successfully constructed by intraperitoneal injection of 8 mg/kg LPS for 12 h.