阴外动脉注射脂多糖对奶牛临床症状及白细胞的影响

为观察泌乳奶牛阴外动脉注射脂多糖(lipopolysaccharides ,LPS)后对临床症状及白细胞(WBC)的影响,选用6头泌乳后期的中国荷斯坦奶牛,用注射LPS处理和空白对照交叉试验设计,每期试验持续一周,期间3头牛进行阴外动脉LPS注射(Escherichia coli 0111:B4,0.01 μg LPS/kg体重),另外3头牛在阴外动脉注射生理盐水。结果表明,与对照组相比,注射LPS极显著影响奶牛的呼吸频率(P＜0.01),在注射后3 h达到最高;注射LPS后对奶牛直肠温度没有影响;注射LPS极显著影响奶牛脉搏(P＜0.01),在注射后6 h达到最高;注射LPS极显著影响奶牛血液中WBC数(P＜0.01),且对其分类计数没有影响。因此,当给奶牛阴外动脉注射LPS后,会影响奶牛的临床症状和WBC数。

Effect of External Pudendal Artery Infusion of Lipopolysaccharide on Clinic Symptoms and White Blood Cells in Dairy Cows

The purpose of this study was to determine the effects of external pudendal artery infusion of lipopolysaccharide(LPS) on clinic symptoms and white blood cells count. Six Chinese Holstein cows were infused with LPS or saline in a crossover design,each period lasted 1 week,three of these cows were infused with LPS(Escherichia coli 0111: B4,0.01 μg LPS/kg body weight) in the external pudendal artery,and the other 3 cows were infused with saline in the external pudendal artery.The results showed that respiratory rate was significantly affected by LPS infusion(P＜0.01),it reached the highest level after 3 h of LPS infusion. Rectal temperature was not affected by LPS infusion, pulse rate was significantly affected by LPS infusion(P＜0.01),it reached the highest level after 6 h of LPS infusion. The white blood cells count was significantly affected by LPS infusion(P＜0.01). So,infusion of LPS affect the clinic symptoms and white blood cells count, and it has no influence on WBC’s differential count.