常规高效液相色谱仪—壳核填料色谱柱快速测定黄曲霉毒素M1

作者使用常规高效液相色谱仪大体积进样技术2.6 μm壳核填料色谱柱快速测定黄曲霉毒素M₁。结果表明，在常规液相色谱仪上使用壳核填料色谱柱比传统全多孔填料色谱柱更加快速有效。与常规全多孔3 μm （4.6 mm×150 mm）和5 μm（4.6 mm×250 mm）填料色谱柱相比，相同仪器条件下使用洗脱强度低于流动相的溶剂定容上机，2.6 μm（4.6 mm×100 mm）核壳结构色谱柱的分析时间分别为后两者的51.5%和21.3%，峰高分别为后两者的183.9%和490.5%，峰方差分别为后两者的34.7%和6.2%。因此，常规HPLC上使用壳核填料色谱柱可以在大幅减少分析时间和成本的同时增加检测的灵敏度和准确性。

Fast Quantification of Aflatoxin M1 with a Conventional HPLC-FLD using a Column Packed with Core-shell Particles

Herein, aflatoxin M₁(AFM₁) was analysed on a conventional HPLC by a chromatography column packed with 2.6 μm core-shell particles and large volumn injection technique. The results show that the core-shell column is faster and more effective than typical total porous particles packed column even on conventional HPLC. With the same instrumental condition and weak injection solvent, the analysis time of new 2.6 μm core-shell particle column (4.6 mm×100 mm) is 51.5% and 21.3% of tranditional total porous 3 μm particle(4.6 mm×150 mm) and 5μm particle(4.6 mm×250 mm) columns respectively. Meanwhile, the peak height is 183.9% and 490.5% of the latter columns and the peak variances is 34.7% and 6.2% of the latter columns. This indicates that the use of core-shell columns on conventional HPLC leads to the reductions of analysis time and cost with increased sensitivity and accuracy.