| HPLC法测定板青颗粒中腺苷含量 |
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| 引用本文： | 钟水燕,杨彦平,付宁静,徐晓曦,徐桃枝,范传园.HPLC法测定板青颗粒中腺苷含量[J].中兽医医药杂志,2010,29(4):54-56. |
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| 作者姓名： | 钟水燕 杨彦平 付宁静 徐晓曦 徐桃枝 范传园 |
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| 作者单位： | 江西中成中药原料有限公司,江西南昌,331700 |
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| 基金项目： | "十一五"国家科技支撑计划"中兽药现代化技术研究与开发(2008BADB4B0206)"资助 |
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| 摘 要： | 目的：建立板青颗粒中腺苷含量的测定方法。方法：采用高效液相色谱法,色谱柱：依利特ODS柱（4.6mm×250mm,15cm）,检测波长：260nm,流动相：甲醇-水（10：90）,柱温：室温,流速：1.0mL/min。结果：腺苷进样量在0.162-1.619μg范围内与峰面积呈良好的线性关系,r=0.9995。平均回收率为99.02%,RSD为0.82%（n=5）。结论：本法操作简便、快速,结果准确可靠。
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| 关 键 词： | 板青颗粒 腺苷 HPLC 含量测定 |
Determination of adenosine contents in Banqing granule by HPLC |
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| ZHONG Shui-yan,YANG Yan-ping,FU Ning-jing,XU Xiao-xi,XU Tao-zhi,FAN Chuan-yuan.Determination of adenosine contents in Banqing granule by HPLC[J].Journal of Traditional Chinese Veterinary Medicine,2010,29(4):54-56. |
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| Authors: | ZHONG Shui-yan YANG Yan-ping FU Ning-jing XU Xiao-xi XU Tao-zhi FAN Chuan-yuan |
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| Institution: | (Jiangxi Zhongcheng Material of Traditional Chinese Medicine Co.,Ltd,Nanchang Jiangxi 331700,China) |
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| Abstract: | Objective：To establish a method for the determination of adenosine contents in Banqing granules.Methods：HPLC was used by chromatographic column of ODS （4.6 mm×250 mm,15 cm）,detective wavelength at 260 nm,the mobile phase of methanol-water （10：90）,column worked at room temperature and the flow rate at 1.0 mL/min.Results：It present a good linear relationship when the sample size of adenosine was within the range of 0.162-1.619 μg,with relationship coefficient of 0.999 5,and the average recovery was 99.02%,RSD was 0.82%（n=5）.Conclusion：The method is convenient,rapid and accurate. |
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| Keywords: | HPLC |
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