| 高效液相法测定复方氟苯尼考注射液中氟苯尼考的含量 |
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| 引用本文： | 杨雪梅,;刘希望,;杨亚军,;李剑勇.高效液相法测定复方氟苯尼考注射液中氟苯尼考的含量[J].中兽医医药杂志,2014(3):39-41. |
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| 作者姓名： | 杨雪梅 ;刘希望 ;杨亚军 ;李剑勇 |
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| 作者单位： | [1]甘肃省金昌市药品检验检测中心,甘肃金昌737100; [2]中国农业科学院兰州畜牧与兽药研究所,甘肃兰州730050 |
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| 基金项目： | 甘肃省支撑计划项目（0804NKCA074） |
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| 摘 要： | 建立了测定氟苯尼考注射液中氟苯尼考含量的高效液相色谱法。采用C18色谱柱（4.6 mm×150 mm,5μm）,流动相为甲醇∶水（pH值为3.0）为40∶60（v/v）,流速为1 mL/min,检测波长为222 nm,进样10μL,柱温为室温。氟苯尼考浓度在0.1-3 mg/mL范围内,峰面积与浓度的线性关系良好（R^2=0.999 6）;平均回收率为99.49%,RSD为1.12%（n=4）。此法简便、快速、灵敏度高、重现性好。
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| 关 键 词： | 芪参超微粉 哈巴俄苷 高效液相色谱法 |
Determination of florfenicol content in florfenicol injection by HPLC |
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| Institution: | YANG Xue-mei, LIU Xi-wang, YANG Ya-jun, LI Jian-yong (1.Jinchang Centre of Drug Inspection and Testing, Jinchang Gansu 737100;2. Lanzhou Institute of Husbandry and Pharmaceutical Science of CAAS, Lanzhou Gansu 730050, China) |
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| Abstract: | A method for the content determination of florfenicolm injection by HPLC was established. Waters C18（4.6 mm×150 mm,5 μm）column was used and the samples were detected by UV detector at 222 nm with water（pH 3.0, adjusted by0.1 mol/L Phosphate）∶methanol（40∶60）as the mobile phase. The flow rate was 1.0 mL/min with the injection volume of 10μL at room temperature. There was no interference occurred from impurities. The calibration curve was linear in the range of 0.1-3 mg/mL（R^2=0.999 6）, and the average recovery was 99.49% with RSD of 1.12%. This established method is simple, rapid, sensitive and accurate. |
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| Keywords: | florfenicol injection content determination HPLC |
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