| HPLC法测定麻杏石甘散中苦杏仁苷的含量 |
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| 引用本文： | 邱天宝,刘素梅,方忠意,班付国,韩立,王丽.HPLC法测定麻杏石甘散中苦杏仁苷的含量[J].中兽医医药杂志,2014(5):40-42. |
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| 作者姓名： | 邱天宝 刘素梅 方忠意 班付国 韩立 王丽 |
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| 作者单位： | 河南省兽药监察所,河南郑州450008 |
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| 摘 要： | 建立HPLC法测定麻杏石甘散中苦杏仁苷含量的方法。色谱柱为Waters XBrige^TM C18（4．6mm×150mm。5μm）；以水-甲醇-乙腈（90．0：1．0：9．0）为流动相；流速：1．0mL／min；柱温：40℃；检测波长：207nm．苦杏仁苷的线性范围为1．6008×104-8．004×104μg，R2=1．0000，平均回收率为97．26％（RSD为0．4％）。本方法快速、简便、准确，能有效测定麻杏石甘散中苦杏仁苷的含量。
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| 关 键 词： | 麻杏石甘散 苦杏仁苷 含量测定 HPLC |
Determination of amygdalin in Ma Xing Shi Gan powder by HPLC |
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| QIU Tian-bao,HU Su-mei,FANG Zhong-yi,BAN Fu-guo,HAN Li,WANG Li.Determination of amygdalin in Ma Xing Shi Gan powder by HPLC[J].Journal of Traditional Chinese Veterinary Medicine,2014(5):40-42. |
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| Authors: | QIU Tian-bao HU Su-mei FANG Zhong-yi BAN Fu-guo HAN Li WANG Li |
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| Institution: | (Henan institute of Vetefinary Drag Control, Zhengzhou Henan 450008, China) |
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| Abstract: | To establish an HPLC method for determination of amygdalin in Ma Xing Shi Gan powder. The amygdalin was analyzed on an XBrigeTM C,s column （4.6 mm ×250 mm, 5 μm） using ultrapure water - methanol - acetonitrite （90.0：1.0：9.0） as the mobile phase. The flow rate was 1.0 mL/min. The column temperature was 40 ℃. The detection wavelength was set at 207 nm. The calibration curve was linear in the range of 1.600 8×10-4-8,004×104 μg （R2=1.000 0） for amygdalin. The recovery of amygdalin was 97.26% with RSD of 0.4%. This method is rapid, simple and accurate, which can be used for the determination of amygdalin in Ma Xing Shi Gan powder. |
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| Keywords: | Ma Xing Shi Gan powder amygdalin determination HPLC |
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