大豆品种(系)抗疫霉根腐病基因的连锁SSR标记分析

 大豆疫霉根腐病是大豆破坏性病害之一。利用抗病品种是防治该病的唯一有效的方法。迄今,已经鉴定了15个抗大豆疫霉根腐病基因(Rps基因),而且大豆部分基因都获得了不同类型的分子标记。本研究根据以前抗病基因推导结果选择26个可能含有Rps1基因座位等位基因或(和)Rps4基因的大豆品种(系),利用分别与Rps1a和Rps4紧密连锁的SSR标记进行抗病基因的分子检测,通过比较含有已知抗病基因对照大豆品种(系)分子标记检测结果并综合以往基因推导结果推断检测品种(系)的Rps基因。在选择的26个品种(系)中,长农14号被证明含有Rps1a,周豆13和铁95068-5含有Rps1a与Rps4基因组合,品系50794、科8924-3和合豆1号含有Rps4;有11个品种(系)存在含有Rps1a的证据、品系50052被推断含有Rps1c与Rps3b基因组合,但不排除这些品种(系)在Rps1座位含有一个新等位基因的可能性。另外,有7个品种(系)的所含抗病基因不能确定,它们可能含有新的Rps基因。

Analysis of Phytophthora resistance loci Rps1 and Rps4 in soybean cultivars or lines using linkage SSR markers

Phytophthora root rot is a destructive disease of soybean.Growing resistant cultivars is the most effective way to control the disease.Up to now,15 Phytophthora resistance genes(Rps gene) have been found,and most of them have been tagged and mapped by using molecular markers.In this study,26 soybean cultivars or lines formerly postulated to carry alleles of Rps1 locus or(and) gene Rps4 were selected to detect Rps genes by using SSR markers linked tightly to Rps1a or Rps4.By comparison of SSR markers linked to known Rps alleles in control cultivars and in combination with the former results of gene postulation,the Rps genes in selected soybean cultivars or lines were proposed.Of 26 cultivars or lines,Changnong14 was likely to possess Rps1a,Zhoudou13 and Tie95068-5 possibly possessed gene combination of Rps1a and Rps4,line 50794,Ke8924-3 and Hedou1 could carry Rps4.Rps1a was detected in 11 cultivars or lines,and line 50052 likely contained a gene combination of Rps1c and Rps3b,but it was not ruled out that these cultivars or lines possibly contained a new allele at the Rps1 locus.In addition,Rps genes in 8 cultivars or lines could not be determined and possibly contain some novel Rps genes.