| 西瓜蔓枯病分子诊断技术研究 |
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| 引用本文： | 戴富明,刘少华,任小杰,陆金萍,倪秀红,徐敬友.西瓜蔓枯病分子诊断技术研究[J].植物病理学报,2006,36(5):439-445. |
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| 作者姓名： | 戴富明 刘少华 任小杰 陆金萍 倪秀红 徐敬友 |
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| 作者单位： | 1 上海市农业科学院植物保护研究所 上海市设施园艺重点实验室, 上海 201106;2 扬州大学农学院植物保护学系, 扬州 225009;3 上海市南汇区农业技术推广中心, 上海 201300 |
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| 基金项目： | 上海市科技兴农科技攻关项目;上海市科技攻关项目 |
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| 摘 要： | 本文测定西瓜上的西瓜蔓枯病菌(Didymella bryoniae)、西瓜炭疽病菌(Colletotrichum orbiculare)及西瓜枯萎病菌(Fusarium oxysporum f. sp. niveum)的rDNA的ITS序列,比对近缘种及西瓜上不同病菌的ITS序列同源性,设计出特异性上游引物XM-2和下游引物XM-R2。经过对XM-2/XM-R2引物的PCR扩增条件的优化,可以扩增出一条344bp的西瓜蔓枯病菌特异性DNA条带。上述方法可以检测到pg以上的蔓枯病菌基因组DNA,并且可以准确扩增出西瓜蔓枯病自然病样中特异性的DNA片段。本文建立了一项西瓜蔓枯病分子检测技术,该方法准确、快速、可靠,可用于西瓜蔓枯病田间的快速诊断。
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| 关 键 词： | 西瓜蔓枯病 西瓜蔓枯病菌 特异性引物 PCR诊断技术 |
| 文章编号： | 0412-0914(2006)05-0439-07 |
| 修稿时间： | 2005年8月5日 |
PCR technique for rapid diagnosis of Didymella bryoniae on watermelon |
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| DAI Fu-ming,LIU Shao-hua,REN Xiao-jie,LU Jin-ping,NI Xiu-hong,XU Jing-you.PCR technique for rapid diagnosis of Didymella bryoniae on watermelon[J].Acta Phytopathologica Sinica,2006,36(5):439-445. |
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| Authors: | DAI Fu-ming LIU Shao-hua REN Xiao-jie LU Jin-ping NI Xiu-hong XU Jing-you |
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| Institution: | 1 Institute of Plant Protection, Shanghai Academy of Agricultural Sciences, Shanghai Key Lab of Protected Horticultural Technology, Shanghai 201106, China;2 Department of Plant Protection, Yangzhou University, Yangzhou 225009, China;3 Shanghai Nanhui Agricultural Technology Extension Centre, Shanghai 201300, China |
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| Abstract: | rDNA ITS sequences of Didymella bryoniae,Colletotrichum orbiculare and Fusarium oxysporium f.sp.niveum were sequenced. A pair of specific primers XM-2 and XM-R2 was designed by homology comparison of ITS sequence of D.bryoniae with closely-related species and other pathogenic fungi from waterme-(lon) and other cucurbitaceous plants.Only a 344 bp DNA band specific to D.bryoniae was amplified with XM-2/XM-R2 primers under the optimized PCR parameters.Above pg level genomic DNA of D.bryoniae could be detected and specific DNA fragments were amplified in the samples of gummy stem blight.A rapid,accurate molecular detection method developed in the study will be useful in the future diagnosis of gummy stem blight of watermelon. |
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| Keywords: | gummy stem blight Didymella bryoniae specific primers PCR diagnosis |
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