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四川省茂县花椒根结线虫病病原鉴定
引用本文:王曦茁, 汪来发, 杨佐忠, 刘子雄, 余明忠.四川省茂县花椒根结线虫病病原鉴定[J].植物保护,2014,40(4):84-88.
作者姓名:王曦茁  汪来发  杨佐忠  刘子雄  余明忠
作者单位:1. 中国林业科学研究院森林生态与保护研究所; 国家林业局森林保护学重点实验室,北京100091; 2. 四川省森林病虫害防治检疫站; 成都610081; 3. 四川省茂县林业局,茂县623200
基金项目:科技部农业科技成果转化资金项目 (2010GB24320615)
摘    要:为明确四川省茂县花椒根结线虫的种类,通过根结线虫雌虫和2龄幼虫的形态特征及雌成虫的会阴花纹、雌虫酯酶同工酶及分子生物学检测(rDNA-ITS区和rRNA-IGS区),对花椒根结线虫进行了种类鉴定。三种方法的结果均表明在茂县地区花椒上发现的根结线虫为北方根结线虫(Meloidogynehapla),这是我国首次报道在花椒上发现北方根结线虫病,四川省是北方根结线虫新的分布区。

关 键 词:北方根结线虫    鉴定    花椒

Identification of the root knot nematode on pepper in Mao County, Sichuan Province
Wang Xizhuo ; Wang Laifa ; Yang Zuozhong ; Liu Zixiong ; Yu Mingzhong.Identification of the root knot nematode on pepper in Mao County, Sichuan Province[J].Plant Protection,2014,40(4):84-88.
Authors:Wang Xizhuo ; Wang Laifa ; Yang Zuozhong ; Liu Zixiong ; Yu Mingzhong
Institution:1. Key Laboratory of Forest Protection of State Forestry Administration; Research Institute of Forest Ecology, Environment and Protection, CAF, Beijing 100091, China; 2. General Station for Forest Disease and Pest Control and Quarantine of Sichuan Province, Chengdu 610081, China; 3. Forestry Bureau of Mao County, Sichuan 623200, China
Abstract:To confirm the nematode species observed on the pepper Zanthoxylum bungeanum in Mao County, Sichuan Province, an investigation was performed in this region from April to September in 2011. Morphological test (perineal patterns and second stage juveniles), biochemical study (esterase isozyme) and molecular characterization (rDNA-ITS and rDNA-IGS) were used to identify the species. The results showed that the pepper root knot nematode belonged to Meloidogyne hapla Chitwood. This is the first report of M. hapla on Z. bungeanum in China and its occurrence in Sichuan Province.
Keywords:
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