合浦珠母贝3个养殖群体的RAPD分析

利用RAPD标记技术检测了广西省北海市、海南省三亚市、广东省深圳市等 3个地理种群养殖合浦珠母贝(Pinctadamartensii)基因组DNA的多态性 ,并对其遗传结构进行了初步分析。从 4 0个随机引物中筛选出 13个10bp引物 ,它们在 3种群中共扩增出了 14 0条DNA片段 ,3种群共有片段 16条 ,DNA片段数分别为 5 8、91和 71条 ,多态性位点比例分别为 4 1 4 % ,6 5 0 %和 5 8 4 %。遗传距离分析表明 ,广西种群与广东种群的亲缘关系较近 ,而与海南种群亲缘关系较远。 13个引物中 ,引物S11和S3 58扩增出的 3种群指纹图谱差异显著 ,可作为 3种群鉴定的分子标记。

RAPD analysis of three cultured populations of Pinctada martensii

Genetic polymorphisms of genomic DNA of three Pinctada martensii populations cultured in the farms of Beihai(Guangxi Province), Sanya Harbor (Hainan Province) and Shenzhen (Guangdong Province) were detected by RAPD technique using 40 random primers. The results show that 13 primers with 10 bp each are effective and each primer gives 1-11 fragments for each sample with the fragment length 100-2 000 bp. One hundred and forty DNA fragments are produced and 16 fragments are generated in the three populations of Pinctada martensii. The fragments numbers of the three populations are 58(Beihai),91(Sanya)and 71(Shenzhen), respectively. The percentages of the polymophic locis are 41 4%, 65 0% and 58 4%, respectively. The genetic distance demonstrates that the lineage of Sanya and Beihai populations is far, and that of Shenzhen and Beihai populations is close. The differences of fingerprintings obtained by primers S 11 and S 358 are so obviously that they can be used as molecular markers.