产低温碱性蛋白酶黄海黄杆菌YS-9412-130的复合诱变原生质体选育

以黄海黄杆菌YS－9412－130突变株SW1－104为出发菌，在原生质体形成和再生的最佳条件下制备原生质体，对原生质体进行复合诱变，对大量再生突变株进行筛选和淡水驯化，最终获得了高产、稳定的碱性蛋白酶产生菌SW2－104，在自来水培养基中能够大量产酶，产酶活力为3910U／ml。从而解决了黄海黄杆菌YS－9412－130低温碱性蛋白酶大规模工业化生产设备和产业化区域的局限性。

The study on selection for strain from F. yellowsea YS-9412-130 producing low-temperature alkaline protease after complex mutagenesis of protoplasts

Taking the mutant F. yellowsea YS 9412 130 SW 1 104 as the initial strain, under the optimum conditions of the formation and regeneration,protoplasts of the strain are prepared, mutagenic treatments of the protoplasts are undergone. After selecting from a large amount of the regenerative mutants and culturing in fresh water, a stable mutant SW 2 104 producing high output alkaline protease is obtained. The strain can produce a large amount of enzyme in tap water medium, enzyme activity is 3 910U/ml. It is possible for low temperature alkaline protease from F. yellowsea YS 9412 130 to solve the problem of large scale production and to break the limitation of industrial region.