微囊藻毒素在鲤体内富集的初步研究

为了解微囊藻毒素MC-LR在鲤各组织器官中的生物富集作用,采用腹腔注射法将MC-LR纯品稀释液(0.215μg/g)注射到鲤体内,酶联免疫吸附法(ELISA)检测0、1、3、12、24和48 h时肾脏、肝脏、肌肉、胆囊、空肠和卵巢中MC-LR含量分布和积累规律.结果显示,MC-LR在肾脏含量最高,均值为(1.007±0.120)μg/g(各器官均按干重计),其次是肝脏(0.490±0.060)μg/g、胆囊(0.355 ±0.011) μg/g、空肠(0.210±0.005)μg/g、卵巢(0.082±0.021)μg/g和肌肉(0.047±0.003) μg/g.鲤不同组织器官对MC-LR的富集能力存在较大差异,肾脏是MC-LR的主要靶器官,卵巢中也存在少量MC-LR富集,肌肉对MC-LR的生物富集量远低于其他组织器官.试验48 h时,鲤体内各组织器官中和鱼缸水中MC-LR含量均有一定程度的下降,表明鲤体内对MC-LR有较强的解毒机制.

Primary Study on Bioaccumulation of Microcystin-LR in Cyprinus carpio

To study the bioaccumulation of microcystin-LR(MC-LR) in different organs in Cyprinus carpio,MCLR contents were measured by the means of ELISA at 1,3,12,24 and 48 h after fish being injected with a dose of 0.215 μg /g body weight MC-LR using intraperitoneal injection.The results showed that MC-LR mean content in kidney was(1.007 ± 0.120) μg /g(dry weight),followed by liver(0.490 ± 0.060) μg /g,gallbladder(0.355 ± 0.011) μg /g,intestine(0.210 ± 0.005) μg /g,ovarian(0.082 ± 0.021) μg /g and muscle(0.047 ± 0.003) μg /g.The bioaccumulation of MC-LR has large differences in C.carpio different organs,with the kidney being the main target organs,while the concentration of microcystin-LR in the muscle and ovarian was relatively low.At 48 h,microcystin-LR content in the analyzed tissues /organs and aquaria water was significantly decreased,which indicated that C.carpio body has a strong detoxifying mechanism for degrading MC-LR.