| DGGE分析不同盐度仿刺参养殖环境中菌群多样性 |
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| 引用本文： | 关晓燕,周遵春,姜冰,董颖,汪笑宇,姜北,杨爱馥,陈仲,王摆,孙大鹏.DGGE分析不同盐度仿刺参养殖环境中菌群多样性[J].水产科学,2011,30(5). |
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| 作者姓名： | 关晓燕 周遵春 姜冰 董颖 汪笑宇 姜北 杨爱馥 陈仲 王摆 孙大鹏 |
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| 作者单位： | 辽宁省海洋水产科学研究院,辽宁省海洋环境监测总站,辽宁省海洋水产分子生物学重点实验室,辽宁,大连,116023 |
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| 基金项目： | 国家海洋局海洋公益专项(200705007); 辽宁省科技计划项目(200820300) |
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| 摘 要： | 采用16S rDNA的PCR-DGGE基因指纹技术对不同盐度仿刺参养殖水环境中的菌群多样性进行了分析。试验设置了22、26、30、34,4个盐度梯度,每隔10 d采样1次,通过PCR-DGGE指纹分析发现,仿刺参养殖环境存在着丰富多样的细菌。其中大多属于非可培养细菌,变形菌门和CFB门的种类是水体中的优势菌群,交替假单胞菌属是报道过的腐皮综合征的致病菌。根据DGGE指纹图谱的聚类分析发现,同等盐度条件下的样品之间存在一定的菌群相似性,并在聚类分析图中分别各自成簇。样品的多样性指数为1.7～2.3,不同盐度条件下,样品的多样性指数随着时间的推移变化不大。
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| 关 键 词： | 仿刺参 盐度 细菌 16S rDNA DGGE指纹 |
Environmental Bacterial Diversity in Sea Cucumber (Apostichopus japonicus) Culture Under Different Salinities Based on DGGE Fingerprint |
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| GUAN Xiao-yan,ZHOU Zun-chun,JIANG Bing,DONG Ying,WANG Xiao-yu,JIANG Bei,YANG Ai-fu,CHEN Zhong,WANG Bai,SUN Da-peng.Environmental Bacterial Diversity in Sea Cucumber (Apostichopus japonicus) Culture Under Different Salinities Based on DGGE Fingerprint[J].Fisheries Science,2011,30(5). |
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| Authors: | GUAN Xiao-yan ZHOU Zun-chun JIANG Bing DONG Ying WANG Xiao-yu JIANG Bei YANG Ai-fu CHEN Zhong WANG Bai SUN Da-peng |
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| Institution: | GUAN Xiao-yan,ZHOU Zun-chun,JIANG Bing,DONG Ying,WANG Xiao-yu,JIANG Bei,YANG Ai-fu,CHEN Zhong,WANG Bai,SUN Da-peng(Liaoning Ocean and Fisheries Science Research Institute,Liaoning Marine Environmental Monitoring General Station,Liaoning Key Lab of Marine Fishery Molecular Biology,Dalian 116023,China) |
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| Abstract: | In this paper,the bacterial diversity in the sea cucumber culture environment at different salinity levels was investigated using a culture-independent method of 16S rDNA PCR-DGGE fingerprinting.The water samples were collected from sea cucumber culture tanks with four salinity gradients(22,26,30,and 34) in a 10 dayinterval.The PCR-DGGE fingerprint analysis showed that the bacteria were abundant in the sea cucumber culture environments,most of which belong to nonculturable bacteria.It was found that species... |
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| Keywords: | sea cucumber salinity bacteria 16S rDNA DGGE fingerprint |
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