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偶氮类色素胭脂红对泥鳅组织酯酶同工酶表达的影响
引用本文:于淑池,张翼,朱叶莲.偶氮类色素胭脂红对泥鳅组织酯酶同工酶表达的影响[J].水产科学,2011,30(8):509-512.
作者姓名:于淑池  张翼  朱叶莲
作者单位:湖州师范学院生命科学学院,浙江 湖州,313000
摘    要:采用肌肉注射法进行染毒,运用聚丙烯酰胺凝胶电泳法研究胭脂红在不同注射剂量、不同染毒时间对泥鳅肝脏、心脏、脾脏、肌肉酯酶同工酶表达的影响。试验结果表明,经一定剂量的胭脂红注射后,泥鳅酯酶同工酶在脾脏中表达最强,肝脏和心脏次之,肌肉最弱,其中脾脏的表达随时间、浓度呈减弱趋势,其余组织则呈先增强再减弱最后增强的趋势。

关 键 词:胭脂红  泥鳅  酯酶同工酶  聚丙烯酰胺凝胶电泳

Effect of Carmine on EST Isozyme Expression in Loach Misgurnus anguillicadatus
YU Shu-chi,ZHANG Yi,ZHU Ye-lian.Effect of Carmine on EST Isozyme Expression in Loach Misgurnus anguillicadatus[J].Fisheries Science,2011,30(8):509-512.
Authors:YU Shu-chi  ZHANG Yi  ZHU Ye-lian
Institution:YU Shu-chi,ZHANG Yi,ZHU Ye-lian(School of Life Science,Huzhou Normal College,Huzhou 313000,China)
Abstract:The influences of carmine on esterase(EST) expressions in hepatopancreas,heart,muscles and spleen of loach Misgurnus anguillicadatus were studied at various doses and exposure period by muscular injection and PAGE.The results showed that the maximal EST expression was found in the spleen after the injection,followed by in the hepatopancreas and the heart.The minimal expression was observed in the muscles.The expression in the spleen was found to decrease as the time proceeded and doses increased,and the exp...
Keywords:carmine  Misgurnus anguillicadatus  isozyme  PAGE  
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