| 海地瓜的分子分类学研究 |
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| 引用本文： | 李慧,李太武,苏秀榕,秦玉明,王孟前,贺静静,胡广洲,黄尚锋,周君.海地瓜的分子分类学研究[J].水产科学,2009,28(12). |
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| 作者姓名： | 李慧 李太武 苏秀榕 秦玉明 王孟前 贺静静 胡广洲 黄尚锋 周君 |
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| 作者单位： | 宁波大学,生命科学与生物工程学院,浙江,宁波,315211 |
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| 基金项目： | 国家农业科技成果转化资金资助项目，宁波市科技成果转化资金资助项目，宁波市科技局资助项目 |
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| 摘 要： | 应用分子系统发育学的方法, 以浙江沿海一海地瓜的18S rDNA和COI片段序列为分子标记,自行设计引物,进行了克隆, 结合来自GenBank 中10种海参的18S rDNA和COI 同源序列, 用邻接法和最大简约法构建系统进化树,并结合形态学特征对它们的分类地位进行了探讨.初步分析结果,基于此海地瓜的各形态学的研究,可以认为它属于海参纲芋参目;鉴于18S rDNA基因的分析,此海地瓜与拟刺参遗传距离为0.017亲缘关系非常近,可以认为它属于海参纲;从COI基因的角度分析,它虽然与怀玉参在系统树上聚成一枝,但支持率很低;仅为49%,遗传距离比较大,为0.223,不足以作为分析的依据.
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| 关 键 词： | 海地瓜 系统进化树 |
The Phylogenetic Relation of Sea Cucumber Acaudina molpadioidea |
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| LI Hui,LI Tai-wu,SU Xiu-rong,QIN Yu-ming,WANG Meng-qian,HE Jing-jing,HU Guang-zhou,HUANG Shang-feng,ZHOU Jun.The Phylogenetic Relation of Sea Cucumber Acaudina molpadioidea[J].Fisheries Science,2009,28(12). |
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| Authors: | LI Hui LI Tai-wu SU Xiu-rong QIN Yu-ming WANG Meng-qian HE Jing-jing HU Guang-zhou HUANG Shang-feng ZHOU Jun |
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| Institution: | LI Hui,LI Tai-wu,SU Xiu-rong,QIN Yu-ming,WANG Meng-qian,HE Jing-jing,HU Guang-zhou,HUANG Shang-feng,ZHOU Jun(Key Laboratory of Applied Technology of Marine Biology,Ministry of Education,Faculty of Life Science , Biotechnology,Ningbo University,Ningbo 315211,China) |
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| Abstract: | The phylogenetic relation of sea cucumber Acaudina molpadioidea from the coast in Zhejiang province were studied by analysis of 1312 bp from 18S rDNA and 692 bp from COImt DNA with homologous sequences of ten species within Holothuroidea downloaded from GenBank as well as the morphological characteristics.Molecular phylogenetic trees were constructed by Neighbor-Joining(NJ)and Maximum Parsimony(MP).The sea cucumber was found to be to Molpadiidae Holothuroidea,inferred from its Bio-Morphotyping.The 18S rDNA ... |
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| Keywords: | 18SrDNA COI Acaudina molpadioidea 18S rDNA molecular phylogenetic trees |
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